Itsn2 (NM_001198969) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR230794
Itsn2 (myc-DDK-tagged) - Mouse intersectin 2 (Itsn2), transcript variant 3
$289.00 $667.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Itsn2
Synonyms AI327390; Ese2; mKIAA1256; Sh3d1B; Sh3p18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001198969
ORF Size 1956 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001198969.2, NP_001185898.1
RefSeq Size 2350 bp
RefSeq ORF 1959 bp
Locus ID 20403
Cytogenetics 12 A1.1
MW 74.3 kDa
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Documents
Resources
"NM_001198969" in other vectors (1)
SKU Description Size Price
MC228583 Itsn2 (untagged) - Mouse intersectin 2 (Itsn2), transcript variant 3 10 ug
$669.00

Plasmid Map for MR230794

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Cloning scheme for MR230794

Circular map for MR230794

file-alt Citations

Extracellular vesicle tetraspanin-8 level predicts distant metastasis in non-small cell lung cancer after concurrent chemoradiation
Liu, Fan, Xu et al
Sci Adv (2020) 6 (11), eaaz6162 DOI: 10.1126/sciadv.aaz6162
Cited by 61
AI Summary: OriGene products, specifically the TSPAN8 cDNA clone (Cat# RC211448) and TSPAN8 antibody (Cat# TA808993), were used in this study to overexpress and detect Tspan8 in NSCLC cell lines and extracellular vesicles, enabling the authors to demonstrate the association between Tspan8 levels and metastatic potential in non-small cell lung cancer.
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Showing 1-1 of 1 papers.
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