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Pitx2 (NM_001042504) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227617
Pitx2 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pitx2
Synonyms 9430085M16Rik; Brx1; Brx1b; Munc30; Otlx2; Ptx2; Rieg
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227617 representing NM_001042504
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCAATTGTCGCAAACTAGTGTCGGCCTGCGTGCAATTAGAGAAAGATAAGGGCCAGCAAGGAA
AGAATGAGGATGTGGGCGCCGAGGACCCGTCCAAGAAGAAGCGGCAACGCCGGCAGAGGACTCATTTCAC
TAGCCAGCAGCTGCAGGAGCTGGAAGCCACTTTCCAGAGAAACCGCTACCCAGACATGTCCACTCGCGAA
GAAATCGCCGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGCCGGGCCAAAT
GGAGAAAGCGGGAACGCAACCAGCAGGCCGAGCTGTGCAAGAATGGCTTTGGGCCGCAGTTCAACGGGCT
CATGCAGCCCTACGATGACATGTACCCCGGCTATTCGTACAACAATTGGGCTGCCAAGGGCCTCACGTCA
GCGTCTCTGTCCACCAAGAGCTTCCCCTTCTTCAACTCCATGAACGTCAATCCCCTGTCCTCTCAGAGTA
TGTTTTCCCCGCCCAACTCCATCTCATCTATGAGTATGTCGTCCAGCATGGTGCCCTCCGCGGTGACCGG
CGTCCCGGGCTCCAGCCTCAATAGCCTGAATAACTTGAACAACCTGAGCAGCCCGTCGCTGAATTCCGCG
GTGCCCACGCCCGCCTGTCCTTACGCGCCGCCGACTCCTCCGTACGTTTATAGGGACACATGTAACTCGA
GCCTGGCCAGCCTGAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGC
CTCCAACCTGAGTGCTTGCCAGTATGCAGTCGACCGGCCGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227617 representing NM_001042504
Red=Cloning site Green=Tags(s)

METNCRKLVSACVQLEKDKGQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTRE
EIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTS
ASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSA
VPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPASNLSACQYAVDRPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042504
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042504.2, NP_001035969.1
RefSeq Size 1759 bp
RefSeq ORF 816 bp
Locus ID 18741
UniProt ID P97474
Cytogenetics 3 57.84 cM
MW 30.8 kDa
Summary Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform Ptx2c is involved in left-right asymmetry the developing embryo.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001042504" in other vectors (4)
SKU Description Size Price
MC209171 Pitx2 (untagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 1, (10ug) 10 ug
$330.00
MG227617 Pitx2 (tGFP-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2) transcript variant 1, (10ug) 10 ug
$489.00 $500.00
MR227617L3 Lenti ORF clone of Pitx2 (Myc-DDK-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 1 10 ug
$600.00
MR227617L4 Lenti ORF clone of Pitx2 (mGFP-tagged) - Mouse paired-like homeodomain transcription factor 2 (Pitx2), transcript variant 1 10 ug
$600.00

Plasmid Map for MR227617

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227617
Circular map for MR227617

file-alt Citations

Mmp14 is required for matrisome homeostasis and circadian rhythm in fibroblasts
Yeung, Garva, Pickard et al
Matrix Biol (2023) 124, 8-22 DOI: 10.1016/j.matbio.2023.10.002
Cited by 16
AI Summary: In this study, the OriGene product "Pitx2 isoform 1 (MR227617)" was used to generate a C-terminal EGFP fusion construct for investigating gene function in fibroblasts.
Snippet: EGFP was cloned at the C terminus of the coding sequence of Pitx2 isoform 1 (Origene;MR227617)
Showing 1-1 of 1 papers.
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