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Slc2a10 (NM_130451) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227535
Slc2a10 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 10 (Slc2a10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $499.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc2a10
Synonyms AA450473; Glut1; Glut10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227535 representing NM_130451
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTTCGCCCAGCTGTCCTCCTGCTCTGTGCCTCTGTGTCTTTGCTGGGGGGCCTGACCTTCGGAT
ATGAGCTGGCCGTCATATCTGGTGCCCTCCTACCACTCCAGCTGAACTTCGGGCTGAGTTGCCTCGAACA
GGAGCTCCTGGTGGGCAGTCTGCTCTTGGGTGCTCTCCTCGCCTCCCTTGTCGGGGGCTTCCTCATTGAC
TGCTATGGCAGGAGACGAGCCATCCTAGGGAGCAATGCGGTGCTGCTGGCTGGCAGCCTGATTCTGGGTC
TGGCCAGCTCCCTGCCCTGGCTGCTCCTGGGCCGTTTATCTGTTGGCTTTGCCATCTCTCTGTCTTCCAT
GGCTTGCTGTATCTACGTGTCAGAATTGGTGGGGCCGCGGCAGAGGGGTGTGCTGGTGTCCCTCTACGAG
GTGGGCATCACTGTGGGCATTCTGTTCTCTTACGGCCTCAACTACGTCCTGGCTGGCAGCCCTTGGGGCT
GGAGGCACATGTTTGGCTGGGCAGCTGCACCTGCTCTCCTACAGTCACTCAGCCTCTTTCTTCTCCCTGC
TGGTGCAGAGGGCACAGCAGCCCCCAAAGACCTTATCCCACTCCAGGGAAGGGAGACAAGCAAACCGGGC
CTGGTGAAGCCACAGTACAGCTTTCTGGACCTCTTCAGGGCCCAGGACGGCATGTGGAGCCGGACTGTAG
TGGGGCTGGGGCTGGTGCTGTTTCAGCAGCTCACAGGACAGCCCAATGTGCTGTATTACGCCTCTACCAT
CTTTCGCTCCGTTGGCTTCCACGGGGGCTCCTCAGCTGTGCTGGCTTCTGTGGGGCTTGGCACGGTGAAG
GTGGCTGCCACCTTGGTCGCCACGGGGCTGGTGGACCGTGCAGGCCGCAGAGTCCTCCTGCTCTTTGGAT
GCGCTCTCATGGCCTTATCGGTCAGCGGCATAGGCCTGGTCAGCTTTGCTGTGTCCCTGGACTCTGGACC
CAGCTGCCTGGCCACATCCAATGCCAGCCAGCAGGTGGATCTGCCTGGAAGCTCGGGTTTGCTTGTGCGT
TCATCTCTGCCGCCAGTGCTACACACCAATGGGGACCAAGGACAGCTGGTCCTGTCAGTCACTGAGAGAC
CCATCCATCCAGTCATCACAGCATCCCTGGGACCAGTTCTGAACACTGCCTCCCCAGTTCCCACCAGTCC
CATCCTTGAGCATACCCTGCTGTGCTGGTCTGCGCTGGTTTGCATGATGGTCTACGTGAGCGCCTTCTCG
GTTGGCTTTGGACCAGTAACCTGGTTGGTCCTCAGTGAGATCTACCCAGCGGAGATCCGAGGGAGAGCCT
TCGCCTTCTGCAGCAGCTTCAACTGGGCAGCTAACCTCTTTATCAGCCTCTCCTTCCTCGACCTGATCGG
TGCCATCGGCTTGGCTTGGACCTTCCTGCTCTATGGGCTGACCGCTGTCCTTGGCCTGGCTTTCATCTAC
CTACTTGTTCCTGAAACCAAAGGACAGTCTTTAGCTGAAATAGAGCAGCAGTTTCAGACAAGCAGGTTCC
CTCTAAACTTTGGCCATAGGCAGCGCATCGGTATTCAGTACCATCGCCTGGATGTCTCCTCAGCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227535 representing NM_130451
Red=Cloning site Green=Tags(s)

MGLRPAVLLLCASVSLLGGLTFGYELAVISGALLPLQLNFGLSCLEQELLVGSLLLGALLASLVGGFLID
CYGRRRAILGSNAVLLAGSLILGLASSLPWLLLGRLSVGFAISLSSMACCIYVSELVGPRQRGVLVSLYE
VGITVGILFSYGLNYVLAGSPWGWRHMFGWAAAPALLQSLSLFLLPAGAEGTAAPKDLIPLQGRETSKPG
LVKPQYSFLDLFRAQDGMWSRTVVGLGLVLFQQLTGQPNVLYYASTIFRSVGFHGGSSAVLASVGLGTVK
VAATLVATGLVDRAGRRVLLLFGCALMALSVSGIGLVSFAVSLDSGPSCLATSNASQQVDLPGSSGLLVR
SSLPPVLHTNGDQGQLVLSVTERPIHPVITASLGPVLNTASPVPTSPILEHTLLCWSALVCMMVYVSAFS
VGFGPVTWLVLSEIYPAEIRGRAFAFCSSFNWAANLFISLSFLDLIGAIGLAWTFLLYGLTAVLGLAFIY
LLVPETKGQSLAEIEQQFQTSRFPLNFGHRQRIGIQYHRLDVSSAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130451
ORF Size 1608 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130451.3, NP_569718.1
RefSeq Size 3434 bp
RefSeq ORF 1611 bp
Locus ID 170441
UniProt ID Q8VHD6
Cytogenetics 2 85.66 cM
MW 57 kDa
Summary This gene encodes a class III facilitative glucose transporter. Mutations in the related gene in human are associated with arterial tortuosity syndrome. provided by RefSeq, Dec 2013
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Documents
Resources
"NM_130451" in other vectors (4)
SKU Description Size Price
MC217632 Slc2a10 (untagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 10 (Slc2a10), (10ug) 10 ug
$550.00
MG227535 Slc2a10 (tGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter) member 10 (Slc2a10), (10ug) 10 ug
$699.00
MR227535L3 Lenti ORF clone of Slc2a10 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 10 (Slc2a10) 10 ug
$799.00
MR227535L4 Lenti ORF clone of Slc2a10 (mGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 10 (Slc2a10) 10 ug
$799.00

Plasmid Map for MR227535

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227535
Circular map for MR227535

file-alt Citations

Glucose Transporter 4 (GLUT4) is Not Necessary for Overload-Induced Glucose Uptake or Hypertrophic Growth in Mouse Skeletal Muscle
McMillin, Schmidt, Kahn et al
Diabetes (2017)
AI Summary: OriGene products, including plasmids for mouse GLUT1 (MR207871), GLUT3 (MR2097915), GLUT6 (MR219710), GLUT10 (MR227535), and a HEK293 cell GLUT10 overexpression lysate (LY410718), were used to study the expression and potential roles of alternative glucose transporters in mouse skeletal muscle during overload-induced glucose uptake.
Snippet: Plasmids containing mouse GLUT1 (catalog #MR207871), GLUT3 (catalog #MR2097915), GLUT6 (catalog #MR219710), and GLUT10 (catalog #MR227535) and an HEK293 cell GLUT10 overexpression lysate (catalog #LY410718) were purchased from OriGene;;... … Plasmids containing mouse GLUT1 (cat#MR207871), GLUT3 (cat#MR2097915), GLUT6 (cat#MR219710) and GLUT10 (cat#MR227535), and a HEK293 cell GLUT10 overexpression lysate (cat#LY410718) were purchased from Origene Technologies. ... …
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