Gsk3a (NM_001031667) Mouse Tagged ORF Clone

SKU MR227461

Gsk3a (Myc-DDK-tagged) - Mouse glycogen synthase kinase 3 alpha (Gsk3a)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Gsk3a
• Synonyms: 2700086H06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227461 representing NM_001031667; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCCGGGCGCGGACCAGCTCGTTCGCGG; AGCCAGGAGGCGGAGGCGGAGGTGGTGGCGGCGGCCCCGGGGGCTCGGCCTCTGGCCCAGGAGGCACTGG; CGGCGGGAAAGCGTCAGTCGGGGCTATGGGTGGGGGCGTGGGAGCCTCGAGCTCCGGGGGTGGCCCCAGC; GGCAGCGGCGGAGGAGGCAGCGGTGGCCCCGGCGCGGGCACTAGCTTCCCGCCGCCGGGAGTGAAGCTGG; GCCGTGACAGCGGGAAGGTGACCACAGTGGTAGCCACTGTAGGCCAAGGCCCAGAGCGTTCCCAAGAAGT; GGCTTACACTGACATCAAAGTGATTGGTAATGGCTCATTCGGAGTAGTATACCAGGCACGGCTGGCAGAG; ACGAGGGAACTGGTGGCCATCAAGAAGGTTCTTCAGGACAAAAGGTTCAAGAACCGAGAACTGCAGATTA; TGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGCGGTACTTTTTCTACTCCAGTGGGGAGAAGAAGGA; CGAGCTGTATTTGAATCTGGTGCTGGAGTATGTGCCCGAGACGGTGTACCGAGTGGCCCGCCACTTCACC; AAGGCCAAGCTGATCACCCCTATCATCTACATCAAGGTGTACATGTACCAGCTCTTCCGGAGCTTGGCCT; ACATCCACTCCCAAGGTGTGTGTCACCGTGACATCAAGCCCCAGAATTTGCTTGTGGACCCTGACACTGC; TGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAGCAGCTGGTTCGGGGGGAGCCCAATGTGTCCTACATC; TGCTCTCGGTACTACCGTGCTCCAGAACTCATCTTTGGAGCCACAGATTACACCTCGTCCATCGATGTGT; GGTCGGCTGGCTGTGTACTTGCTGAGCTACTTCTCGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGA; CCAGCTTGTGGAGATCATCAAGGTACTAGGAACGCCAACCAGGGAACAAATCCGAGAGATGAACCCTAAC; TATACGGAGTTCAAGTTCCCCCAGATCAAAGCTCACCCTTGGACAAAGGTGTTCAAATCTTCAAAGACAC; CACCTGAGGCCATTGCACTCTGCTCTAGCCTACTGGAGTACACGCCATCCTCAAGGCTCTCCCCACTCGA; GGCTTGTGCCCACAGCTTCTTCGATGAACTGCGGAGACTCGGAGCCCAGCTCCCCAACGACCGCCCGCTT; CCCCCCCTGTTCAACTTCAGTCCTGGTGAACTGTCCATCCAACCATCTCTCAATGCCATTCTCATCCCTC; CTCACTTGAGGTCCCCAGCAGGCCCTGCTTCTCCCCTCACCACTTCCTACAACCCATCCTCACAAGCTTT; AACTGAAGCTCAGACTGGCCAAGATTGGCAGCCATCTGATGCCACAACTGCTACCCTCGCTAGCTCTTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227461 representing NM_001031667; Red=Cloning site Green=Tags(s); ; MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPS; GSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATVGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAE; TRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFT; KAKLITPIIYIKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYI; CSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPN; YTEFKFPQIKAHPWTKVFKSSKTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRRLGAQLPNDRPL; PPLFNFSPGELSIQPSLNAILIPPHLRSPAGPASPLTTSYNPSSQALTEAQTGQDWQPSDATTATLASSS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031667
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001031667.1, NP_001026837.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1473 bp
• Locus ID: 606496
• UniProt ID: Q2NL51
• Cytogenetics: 7 A3
• MW: 52.1 kDa
• Summary: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 (PubMed:15791206, PubMed:17908561). Requires primed phosphorylation of the majority of its substrates (PubMed:22539723). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:15791206, PubMed:17908561). Regulates glycogen metabolism in liver, but not in muscle (PubMed:17908561). May also mediate the development of insulin resistance by regulating activation of transcription factors (By similarity). In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin (PubMed:15791206). Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease (By similarity). May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (PubMed:17391670). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (PubMed:16543145). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed:22539723).[UniProtKB/Swiss-Prot Function]