Kcnj10 (NM_001039484) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR227234
Kcnj10 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10)
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Kcnj10 |
| Synonyms | BIR10; BIRK-1; Kir1.2; Kir4.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_001039484 |
| ORF Size | 1137 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001039484.1, NP_001034573.1 |
| RefSeq Size | 5407 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 16513 |
| UniProt ID | Q9JM63 |
| Cytogenetics | 1 79.69 cM |
| MW | 42.9 kDa |
| Summary | May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001039484" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208801 | Kcnj10 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10), (10ug) | 10 ug |
$732.00
|
|
| MG227234 | Kcnj10 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel subfamily J member 10 (Kcnj10), (10ug) | 10 ug |
$886.00
|
|
| MR227234L1 | Lenti ORF clone of Kcnj10 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) | 10 ug |
$986.00
|
|
| MR227234L2 | Lenti ORF clone of Kcnj10 (mGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) | 10 ug |
$986.00
|
|
| MR227234L3 | Lenti ORF clone of Kcnj10 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) | 10 ug |
$986.00
|
|
| MR227234L4 | Lenti ORF clone of Kcnj10 (mGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) | 10 ug |
$986.00
|
|
Citations
Identification of Aethina tumida Kir Channels as Putative Targets of the Bee Venom Peptide Tertiapin Using Structure-Based Virtual Screening Methods
Doupnik
Toxins (Basel) (2019) 11 (9) DOI: 10.3390/toxins11090546
Doupnik
Toxins (Basel) (2019) 11 (9) DOI: 10.3390/toxins11090546
AI Summary: OriGene Technologies contributed to this study by providing the pCMV6 vector containing mouse Kir4.1 cDNA with a Myc-DDK tag (NM_001039484), which was used for in vitro transcription and functional analysis of Kir channels.
Snippet: oocytes were injected with cRNA transcribed in vitro by T7 RNA polymerase (mMessage mMachine, Ambion, Austin, TX, USA) from a linearized pCMV6 vector with the mouse Kir4.1 cDNA containing a Myc-DDK tag at the C-terminus (NM_001039484, Origene Technologies Cached
Showing 1-1 of 1 papers.
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