OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Irs2 (NM_001081212) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR227167
Irs2 (Myc-DDK-tagged) - Mouse insulin receptor substrate 2 (Irs2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,150.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Irs2 or NM_001081212"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Irs2
Synonyms Irs-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227167 representing NM_001081212
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGGGGACGGCCCGAACCTCAATAACAACA
ACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCGCTT
TTTCGTGTTGCGCGGCCCCGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGGGTCGCCGCCGCAGCCT
CCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGTGGAGGAGCAAGGCGGGCGCGCCGAAGCGAGTGATCG
CGCTCGACTGCTGTCTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCTCTACAC
CAAGGACGAGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCACTCACC
GACTTGGTCAGCGAAGGCCGCTCTGGCGAGGGGGGCTCGGGCACCACCGGAGGCTCTTGCAGCGCCTCTC
TCCCGGGCGTCCTGGGCGGCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACGGGCTCGTGACACCCGC
CACGGCCGTCTACCGCGAGGTGTGGCAGGTGAACCTGAAACCTAAGGGACTGGGCCAGAGCAAGAACCTG
ACTGGTGTATACCGCCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAACAGCCGT
CGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCGGACAGCTTCTTCTTCATCGAGGTGGG
CCGTTCGGCCGTCACCGGTCCCGGGGAGCTGTGGATGCAAGCCGACGACTCGGTGGTGGCGCAGAACATC
CATGAGACCATCCTAGAAGCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGGCCTCGCAGCAAGAGTC
AGTCGTCCGGGTCGTCAGCCACGCATCCCATCAGCGTGCCGGGCGCGCGCCGCCACCACCACCTAGTCAA
CCTACCCCCTAGCCAGACCGGCCTGGTGCGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCCCCAGCA
GCCAAGTGCACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGACGGCGGCGCGGCAGGCGGGGCCGGGA
CGGCAGGAGGCAGGCCGATGTCGGTGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAG
CCGCTCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCTGGCGCCGGCAGGGGGA
GCCCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCACCTCCTGCAGCCACCAGCCCAGGCA
GCCTGTCCTCCAGCAGTGGGCACGGCTCGGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCACCTGCC
TCATCCACTGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTAGTGCCTCCGCCTCGGGCTCCCCCAGC
GACCCGGGTTTCATGTCCCTTGACGAGTATGGCTCCAGCCCTGGCGACCTGAGAGCCTTCAGTAGCCACA
GGAGCAACACACCCGAGTCAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTGGGGGCGAACTCTATGG
GTACATGAGCATGGATAGACCCCTGAGCCACTGTGGCCGCCCTTACCGTAGGGTCTCAGGGGATGGGGCC
CAGGACCTGGATAGAGGACTGAGGAAGAGGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGCAGGTAC
CTCAGCCTTCCTCTGCCTCTCTAGATGAATACACTCTCATGAGGGCCACCTTCTCTGGTAGTTCAGGTCG
CCTCTGCCCATCCTTCCCTGCGTCCTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGAGAC
ATTGAGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGCTACATGCCCATGACCC
CTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAGCTGCAAGAGCGATGACTACATGCCCATGAGCCCCAC
AAGCGTGTCTGCTCCCAAGCAGATCCTGCAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGAGCAGCC
GTGCCAGCACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAACGGAGGTGGCTACAAAGCCAGCTCCC
CAGCGGAGAGCTCCCCAGAAGACAGTGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCTATGGAGAA
CCCAGACCCTAAGCTACTCCCCAACGGGGACTACCTCAACATGTCCCCCAGCGAGGCAGGCACTGCAGGG
ACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAGGCAGTGAAGGCCTCAAAGGCATCCCGGGCCACTGCT
ACAGCTCTTTGCCCCGCTCTTATAAGGCTCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGTGCTCAT
GAGCTCCCCTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGCCCCAGGCCACCCCAGGGGCTGGCACC
TTTGGGGCAGCTGGTGGTAGTCATACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGAGGCCGAGTG
CCATCGGTGGCCGCCCTGAGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGCCTACACGCCTATCGCT
AGAGGGACTGCAGACCCTTCCCAGCATGCAAGAGTACCCTCTACCCACAGAGCCCAAGAGCCCTGGCGAG
TACATCAACATTGACTTTGGTGAGGCAGGTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGGCATCCG
CGGCCTCATCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCATCCCTGGGTTCAGGAACCCCAGGCAC
CAGCAGCGACAGCCGGCAGCGCTCTCCACTCTCTGACTATATGAACCTGGACTTCAGTTCTCCCAAGTCC
CCCAAGCCTAGCACCCGCAGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTCTCTCCAGAGGCTTCAT
CCCCATACCCACCACTGCCCCCACGTCCTTCCACTTCCCCTTCCTCCTTACAGCAGCCTCTGCCACCTGC
CCCGGGAGACCTATACCGCCTGCCTCCAGCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGCTCCTCA
ATGTCCTCCGAGCCTGGGGATAATGGTGACTATACCGAGATGGCCTTTGGTGTGGCTGCAACCCCGCCAC
AACCTATCGTGGCACCTCCAAAGCCAGAAGGTGCCCGAGTGGCCAGTCCCACATCGGGCTTGAAGCGGCT
AAGTCTCATGGATCAGGTATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCCCCCTGACCCCCACCGG
GGTGCTAAGGTCATCCGTGCAGACCCACAGGGGGGACGTCGTCGCCACAGTTCAGAGACCTTTTCCTCTA
CCACCACCGTCACCCCAGTGTCCCCATCCTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTCTGTGGA
AAATGTCTCACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGGGAGGAGGTGATGAGCCGCCCACATCC
CCAGGACAGGCACAGCCCTTGGTGGCTGTGCCCCCAGTGCCACAGGCTAGGCCGTGGAACCCCGGTCAGC
CCGGAGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGACCTCCGTGGGTTTCCA
GAACGGCCTCAACTATATCGCCATCGATGTGAGAGGCGAGCAGGGGTCCTTGGCGCAGTCTCAGCCGCAG
CCAGGAGACAAGAACTCCTGGAGCCGGACCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAGGCTCTG
GCGCCAGCGGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCCTCTGCCAGCACCTATGCAAGCATCGA
CTTCCTGTCCCATCACTTGAAGGAAGCCACAGTCGTGAAAGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227167 representing NM_001081212
Red=Cloning site Green=Tags(s)

MASAPLPGPPASAGGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGTGGDEASAAGGSPPQP
PRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALT
DLVSEGRSGEGGSGTTGGSCSASLPGVLGGSAGAAGCDDNYGLVTPATAVYREVWQVNLKPKGLGQSKNL
TGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNI
HETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPA
AKCTSCRVRTASEGDGGAAGGAGTAGGRPMSVAGSPLSPGPVRAPLSRSHTLSAGCGGRPSKVTLAPAGG
ALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPLPPGSHPHLPHPLHHPQGQRPSSGSASASGSPS
DPGFMSLDEYGSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGYMSMDRPLSHCGRPYRRVSGDGA
QDLDRGLRKRTYSLTTPARQRQVPQPSSASLDEYTLMRATFSGSSGRLCPSFPASSPKVAYNPYPEDYGD
IEIGSHKSSSSNLGADDGYMPMTPGAALRSGGPNSCKSDDYMPMSPTSVSAPKQILQPRLAAALPPSGAA
VPAPPSGVGRTFPVNGGGYKASSPAESSPEDSGYMRMWCGSKLSMENPDPKLLPNGDYLNMSPSEAGTAG
TPPDFSAALRGGSEGLKGIPGHCYSSLPRSYKAPCSCSGDNDQYVLMSSPVGRILEEERLEPQATPGAGT
FGAAGGSHTQPHHSAVPSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLEGLQTLPSMQEYPLPTEPKSPGE
YINIDFGEAGTRLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKS
PKPSTRSGDTVGSMDGLLSPEASSPYPPLPPRPSTSPSSLQQPLPPAPGDLYRLPPASAATSQGPTAGSS
MSSEPGDNGDYTEMAFGVAATPPQPIVAPPKPEGARVASPTSGLKRLSLMDQVSGVEAFLQVSQPPDPHR
GAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNSKRHNSASVENVSLRKSSEGSSTLGGGDEPPTS
PGQAQPLVAVPPVPQARPWNPGQPGALIGCPGGSSSPMRRETSVGFQNGLNYIAIDVRGEQGSLAQSQPQ
PGDKNSWSRTRSLGGLLGTVGGSGASGVCGGPGTGALPSASTYASIDFLSHHLKEATVVKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081212
ORF Size 3963 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081212.2
RefSeq Size 4015 bp
RefSeq ORF 3966 bp
Locus ID 384783
UniProt ID P81122
Cytogenetics 8 5.35 cM
MW 137.2 kDa
Summary May mediate the control of various cellular processes by insulin.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001081212" in other vectors (4)
SKU Description Size Price
MC224254 Irs2 (untagged) - Mouse insulin receptor substrate 2 (Irs2), (10ug) 10 ug
$1,266.00
MG227167 Irs2 (tGFP-tagged) - Mouse insulin receptor substrate 2 (Irs2), (10ug) 10 ug
$1,350.00
MR227167L3 Lenti ORF clone of Irs2 (Myc-DDK-tagged) - Mouse insulin receptor substrate 2 (Irs2) 10 ug
$1,450.00
MR227167L4 Lenti ORF clone of Irs2 (mGFP-tagged) - Mouse insulin receptor substrate 2 (Irs2) 10 ug
$1,450.00

Plasmid Map for MR227167

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227167
Circular map for MR227167

file-alt Citations

MicroRNA-203a regulates pancreatic β cell proliferation and apoptosis by targeting IRS2
Duan, Zhao, Jin et al
Mol. Biol. Rep. (2020)
Cited by 6
AI Summary: OriGene’s Irs2 expression plasmid was used to overexpress IRS2 in MIN6 pancreatic β cells, enabling the authors to study the effects of IRS2 on cell proliferation and apoptosis in the context of miR-203a regulation.
Snippet: analysis. The Irs2 expression plasmid was purchased from OriGene (Beijing, China) and was transfected into MIN6 cells using the Attractene Transfection Reagent (Qiagen, Germany). Quantitative real-time PCR. Total cellular
Oncogenic ERG represses PI3K signaling through down-regulation of IRS2
Mao, Gao, Hu et al
Cancer Res. (2020)
AI Summary: OriGene products contributed to this study by providing the Mouse IRS-2 cDNA (MR227167) and the Myc-DDK-tagged Lenti plasmid (PS100092), which were used to analyze the effects of IRS2 overexpression on PI3K signaling in prostate cancer models.
Snippet: Tech;4390844). Stable gene expression analysis: Mouse IRS-2 (Origene;MR227167) was cloned into a Myc-DDK-tagged Lenti plasmid (Origene;PS100092). Inducible gene expression by doxycycline: pCW-CFP and pCW-CFP-ERG were transfected into 293FT
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products