Kl (NM_013823) Mouse Tagged Lenti ORF Clone
$1,625.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Kl |
| Synonyms | alpha-kl |
| Vector | pLenti-C-mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013823 |
| ORF Size | 3042 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013823.2, NP_038851.2 |
| RefSeq Size | 5124 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 16591 |
| UniProt ID | O35082 |
| Cytogenetics | 5 G3 |
| Summary | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013823" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223205 | Kl (untagged) - Mouse klotho (Kl), (10ug) | 10 ug |
$1,325.00
|
|
| MG227101 | Kl (tGFP-tagged) - Mouse klotho (Kl), (10ug) | 10 ug |
$1,525.00
|
|
| MR227101 | Kl (Myc-DDK-tagged) - Mouse klotho (Kl) | 10 ug |
$1,325.00
|
|
| MR227101L1 | Lenti ORF clone of Kl (Myc-DDK-tagged) - Mouse klotho (Kl) | 10 ug |
$1,625.00
|
|
| MR227101L3 | Lenti ORF clone of Kl (Myc-DDK-tagged) - Mouse klotho (Kl) | 10 ug |
$1,625.00
|
|
| MR227101L4 | Lenti ORF clone of Kl (mGFP-tagged) - Mouse klotho (Kl) | 10 ug |
$1,625.00
|
|
Citations
Klotho overexpression improves amyloid-β clearance and cognition in the APP/PS1 mouse model of Alzheimer's disease
Zhao, Zeng, Li et al
Aging Cell (2020)
Zhao, Zeng, Li et al
Aging Cell (2020)
Cited by 137
AI Summary: OriGene products, specifically the GFP-tagged lentiviral plasmid carrying full-length mouse Klotho cDNA (LV-KL) and shRNA against human Klotho (shKlotho), were used to manipulate Klotho expression in both mouse and human cell models, enabling the study to assess the effects of Klotho on amyloid-β clearance and cognitive function in Alzheimer's disease.
Snippet: nd lentivirus preparation Green fluorescent protein (GFP)‐tagged lentiviral plasmid carrying a full‐length mouse Klotho cDNA (LV‐KL) or shRNA against human Klotho (shKlotho) were purchased from OriGene Technologies, supplemented with a negative control (LV‐GFP or shNC, respectively). … Th;Green fluorescent protein (GFP)‐tagged lentiviral plasmid carrying a full‐length mouse Klotho cDNA (LV‐KL) or shRNA against human Klotho (shKlotho) were purchased from OriGene Technologies, supplemented with a negative control (LV‐GFP or shNC, respectively). …
Lentiviral vector-mediated overexpression of Klotho in the brain improves Alzheimer's disease-like pathology and cognitive deficits in mice
Zeng, Yang, Zhou et al
Neurobiol Aging (2019) 78, 18-28 DOI: 10.1016/j.neurobiolaging.2019.02.003
Zeng, Yang, Zhou et al
Neurobiol Aging (2019) 78, 18-28 DOI: 10.1016/j.neurobiolaging.2019.02.003
Cited by 87
AI Summary: In this study, OriGene’s anti-Klotho antibody (TA336233) and anti-β-actin antibody (TA-09) were used for Western blot analysis to assess protein expression levels, thereby contributing to the evaluation of Klotho overexpression and its effects in the Alzheimer’s disease mouse model.
Lentiviral-mediated klotho up-regulation improves aging-related memory deficits and oxidative stress in senescence-accelerated mouse prone-8 mice
Zhou, Zeng, Yang et al
Life Sci. (2018)
Zhou, Zeng, Yang et al
Life Sci. (2018)
AI Summary: OriGene’s Lenti ORF clone of KL (mGFP-tagged) encoding the full-length mouse Klotho was used to deliver and sustain klotho gene expression in mice, enabling the study of its therapeutic effects on aging-related memory deficits and oxidative stress.
Snippet: The Lenti ORF clone of KL (mGFP-tagged) - Mouse KLotho (encoding the transmembrane full-length form of klotho) was obtained from OriGene Technologies (Rockville, MD, USA) and the vector was obtained from BGI (Shenzhen, Guangdong, China)
Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition of RIG-I/NF-κB Signaling
Zhou, Li, Shi et al
Front Pharmacol (2017) 8, 950 DOI: 10.3389/fphar.2017.00950
Zhou, Li, Shi et al
Front Pharmacol (2017) 8, 950 DOI: 10.3389/fphar.2017.00950
AI Summary: OriGene supplied the lentiviral vectors encoding mouse Klotho (LV-KL), rat Klotho shRNA (LV-KL shRNA), and control GFP (LV-GFP), which were essential for manipulating Klotho expression in the study to investigate its protective effects against ischemic brain injury.
Snippet: Lentiviral vectors that encoded the transmembrane form of mouse Klotho (LV-KL) and harbored a short-hairpin RNA sequence that targeted rat Klotho (LV-KL shRNA) and corresponding control lentiviral vectors that encoded green florescence protein (LV-GFP) were obtained from OriGene (Rockville, MD, United States). … When HEK293 cells (ATCC, Manassas, VA, United States) reached 90–100% confluence, GM easyTM lentiviral mix (Genomeditech, Shanghai, China), lipofectamine 3000 (Invitrogen, Carlsbad, CA, United States), and LV-KL or LV-GFP were mixed in serum-free, antibiotic-free Dulbecco’s Modified Eagle Medium and then added to the culture dishes together with fresh culture medium
Showing 1-4 of 4 papers.
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