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Fcer1a (NM_010184) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226932
Fcer1a (Myc-DDK-tagged) - Mouse Fc receptor, IgE, high affinity I, alpha polypeptide (Fcer1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Fcer1a or NM_010184"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fcer1a
Synonyms Fce1a; fcepsilonri; FcERI; Fcr-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226932 representing NM_010184
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCACTGGAAGGTCTGCCCAGCTGTGCCTAGCACTGCTGTTCATGTCTCTTGATGTCATTCTGACAG
CCACTGAGAAATCTGTACTGACCTTGGACCCACCATGGATTAGAATATTTACAGGAGAGAAAGTGACCCT
TTCCTGCTATGGGAACAATCACCTTCAAATGAACTCTACTACTAAATGGATCCACAATGGTACCGTCTCT
GAGGTGAACTCTTCACATTTGGTCATTGTGAGTGCCACCGTTCAAGACAGTGGAAAATACATATGTCAGA
AGCAAGGATTGTTTAAGAGTAAACCTGTGTACTTGAATGTAACGCAAGATTGGCTGCTCCTTCAGACATC
TGCTGACATGATCTTAGTCCATGGATCCTTTGACATCAGATGCCATGGCTGGAAGAACTGGAATGTCCGC
AAGGTGATCTACTACAGGAATGACCATGCTTTCAACTACAGTTATGAGAGCCCCGTCTCCATTAGAGAGG
CCACACTGAATGACAGTGGCACCTACCACTGCAAGGGCTATCTTAGGCAGGTGGAATATGAATCTGACAA
ATTCAGAATTGCTGTAGTAAAAGCTTACAAATGCAAGTATTATTGGCTACAACTAATTTTCCCATTGTTG
GTGGCGATTCTGTTTGCTGTGGACACGGGGTTATTGCTCTCAACCGAAGAACAGTTCAAATCAGTCTTGG
AGATTCAGAAGACTGGAAAATACAAGAAAGTTGAAACCGAACTCCTAACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010184
ORF Size 750 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010184.1
RefSeq Size 1026 bp
RefSeq ORF 753 bp
Locus ID 14125
UniProt ID P20489
Cytogenetics 1 80.33 cM
MW 29.1 kDa
Summary Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.UniProtKB/Swiss-Prot Function
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"NM_010184" in other vectors (4)
SKU Description Size Price
MC208469 Fcer1a (untagged) - Mouse Fc receptor, IgE, high affinity I, alpha polypeptide (Fcer1a), (10ug) 10 ug
$300.00
MG226932 Fcer1a (tGFP-tagged) - Mouse Fc receptor IgE high affinity I alpha polypeptide (Fcer1a), (10ug) 10 ug
$489.00 $500.00
MR226932L3 Lenti ORF clone of Fcer1a (Myc-DDK-tagged) - Mouse Fc receptor, IgE, high affinity I, alpha polypeptide (Fcer1a) 10 ug
$600.00
MR226932L4 Lenti ORF clone of Fcer1a (mGFP-tagged) - Mouse Fc receptor, IgE, high affinity I, alpha polypeptide (Fcer1a) 10 ug
$600.00

Plasmid Map for MR226932

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226932
Circular map for MR226932

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