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Fgfr3 (NM_001163217) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226920
Fgfr3 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 4
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $734.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fgfr3
Synonyms CD333; Fgfr-; Fgfr-3; Flg-2; FR3; HBGF; HBGFR; Mfr3; sa; sam3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226920 representing NM_001163217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAGTCCCGGCCTGCGTGCTAGTGTTCTGCGTGGCGGTCGTGGCTGGAGCTACTTCCGAGCCTCCTG
GTCCAGAGCAGCGAGTTGTGCGGAGAGCGGCAGAGGTTCCAGGGCCTGAACCTAGCCAGCAGGAGCAGGT
GGCCTTCGGCAGTGGGGACACCGTGGAGCTGAGCTGCCATCCTCCTGGAGGTGCCCCCACAGGGCCCACG
GTCTGGGCTAAGGATGGTACAGGTCTGGTGGCCTCCCACCGCATCCTGGTGGGGCCTCAGAGGCTGCAAG
TGCTAAATGCCTCCCACGAAGATGCAGGGGTCTACAGCTGCCAGCACCGGCTCACTCGGCGTGTGCTGTG
CCACTTCAGTGTGCGTGTAACAGATGCTCCATCCTCAGGAGATGACGAAGATGGGGAGGACGTGGCTGAA
GACACAGGGGCTCCTTATTGGACTCGCCCGGAGCGAATGGATAAGAAACTGCTGGCTGTGCCAGCCGCAA
ACACTGTCCGCTTCCGCTGCCCAGCTGCTGGCAACCCTACCCCCTCCATCTCCTGGCTGAAGAATGGCAA
AGAATTCCGAGGGGAGCATCGCATTGGGGGCATCAAGCTCCGGCACCAGCAGTGGAGCTTGGTCATGGAA
AGTGTGGTACCCTCCGATCGTGGCAACTATACCTGTGTAGTTGAGAACAAGTTTGGCAGCATCCGGCAGA
CATACACACTGGATGTGCTGGAGCGCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCA
GACAGCCATTCTAGGCAGTGACGTGGAGTTCCACTGCAAGGTGTACAGCGATGCACAGCCACACATCCAG
TGGCTGAAGCACGTGGAAGTGAACGGCAGCAAGGTGGGCCCTGACGGCACGCCCTACGTCACTGTACTCA
AGTCCTGGATCAGTGAGAATGTGGAGGCAGACGCACGCCTCCGCCTGGCCAATGTGTCGGAGCGGGACGG
GGGCGAGTACCTCTGTCGAGCCACCAATTTCATAGGCGTGGCTGAGAAGGCCTTTTGGCTGCGTGTTCAC
GGGCCCCAAGCAGCTGAGGAGGAGCTGATGGAAACTGATGAGGCTGGCAGCGTGTACGCAGGCGTCCTCA
GCTACGGGGTGGTCTTCTTCCTCTTCATCCTGGTGGTGGCAGCTGTGATACTCTGCCGCCTGCGCAGTCC
CCCAAAGAAGGGCTTGGGCTCGCCCACCGTGCACAAGGTCTCTCGCTTCCCGCTTAAGCGACAGGTGTCC
TTGGAATCTAACTCCTCTATGAACTCCAACACACCCCTTGTCCGGATTGCCCGGCTGTCCTCAGGAGAAG
GTCCTGTTCTGGCCAATGTTTCTGAACTTGAGCTGCCTGCTGACCCCAAGTGGGAGCTATCCAGGACCCG
GCTGACACTTGGTAAGCCTCTTGGAGAAGGCTGCTTTGGACAGGTGGTCATGGCAGAAGCTATTGGCATC
GACAAGGACCGTACTGCCAAGCCTGTCACCGTGGCCGTGAAGATGCTGAAAGATGATGCGACTGACAAGG
ACCTGTCGGACCTGGTATCTGAGATGGAGATGATGAAAATGATTGGCAAGCACAAGAACATCATTAACCT
GCTGGGGGCGTGCACACAGGGTGGGCCCCTGTATGTGCTGGTGGAGTACGCAGCCAAGGGCAATCTCCGG
GAGTTCCTTCGGGCGCGGCGGCCTCCAGGCATGGACTACTCCTTTGATGCCTGCAGGCTGCCAGAGGAAC
AGCTCACCTGCAAGGATCTAGTGTCCTGTGCCTACCAGGTGGCACGGGGCATGGAATACTTGGCTTCTCA
GAAGTGTATTCACAGAGACTTGGCTGCCAGAAACGTCCTGGTGACCGAGGACAATGTGATGAAGATTGCG
GACTTTGGCCTGGCTCGAGATGTGCACAACCTGGACTACTACAAGAAGACCACAAATGGCCGGCTACCTG
TGAAGTGGATGGCACCAGAGGCCCTTTTTGACCGAGTCTACACCCACCAGAGTGATGTTTGGTCTTTTGG
TGTCCTCCTCTGGGAGATCTTTACGCTGGGGGGCTCACCGTATCCTGGCATCCCAGTGGAAGAGCTTTTC
AAGCTGTTGAAAGAGGGCCACCGCATGGACAAGCCAGCCAGCTGCACACATGACCTGTACATGATCATGC
GGGAATGTTGGCATGCGGTGCCTTCACAGAGGCCCACCTTCAAGCAGTTGGTAGAGGATTTAGACCGCAT
CCTCACTGTGACATCAACCGACGAGTACTTGGACCTCTCCGTGCCGTTTGAGCAGTACTCGCCAGGTGGC
CAGGACACGCCTAGCTCCAGCTCGTCCGGAGATGACTCGGTGTTCACCCATGACCTGCTACCCCCAGGTC
CACCCAGTAACGGGGGACCTCGGACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226920 representing NM_001163217
Red=Cloning site Green=Tags(s)

MVVPACVLVFCVAVVAGATSEPPGPEQRVVRRAAEVPGPEPSQQEQVAFGSGDTVELSCHPPGGAPTGPT
VWAKDGTGLVASHRILVGPQRLQVLNASHEDAGVYSCQHRLTRRVLCHFSVRVTDAPSSGDDEDGEDVAE
DTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGKEFRGEHRIGGIKLRHQQWSLVME
SVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAILGSDVEFHCKVYSDAQPHIQ
WLKHVEVNGSKVGPDGTPYVTVLKSWISENVEADARLRLANVSERDGGEYLCRATNFIGVAEKAFWLRVH
GPQAAEEELMETDEAGSVYAGVLSYGVVFFLFILVVAAVILCRLRSPPKKGLGSPTVHKVSRFPLKRQVS
LESNSSMNSNTPLVRIARLSSGEGPVLANVSELELPADPKWELSRTRLTLGKPLGEGCFGQVVMAEAIGI
DKDRTAKPVTVAVKMLKDDATDKDLSDLVSEMEMMKMIGKHKNIINLLGACTQGGPLYVLVEYAAKGNLR
EFLRARRPPGMDYSFDACRLPEEQLTCKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTEDNVMKIA
DFGLARDVHNLDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLLWEIFTLGGSPYPGIPVEELF
KLLKEGHRMDKPASCTHDLYMIMRECWHAVPSQRPTFKQLVEDLDRILTVTSTDEYLDLSVPFEQYSPGG
QDTPSSSSSGDDSVFTHDLLPPGPPSNGGPRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163217
ORF Size 2406 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163217.2, NP_001156689.1
RefSeq Size 4033 bp
RefSeq ORF 2409 bp
Locus ID 14184
Cytogenetics 5 17.83 cM
MW 88.6 kDa
Summary This gene encodes a member of the fibroblast growth factor receptor family. Members of this family are highly conserved proteins that differ from one another in their ligand affinities and tissue distribution. A representative protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene may be associated with craniosynostosis and multiple types of skeletal dysplasia. A pseudogene of this gene is located on chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Apr 2011
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Documents
Resources
"NM_001163217" in other vectors (4)
SKU Description Size Price
MC221692 Fgfr3 (untagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 4, (10ug) 10 ug
$809.00
MG226920 Fgfr3 (tGFP-tagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3) transcript variant 4, (10ug) 10 ug
$934.00
MR226920L3 Lenti ORF clone of Fgfr3 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 4 10 ug
$1,034.00
MR226920L4 Lenti ORF clone of Fgfr3 (mGFP-tagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 4 10 ug
$1,034.00

Plasmid Map for MR226920

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226920
Circular map for MR226920

file-alt Citations

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