Lepr (NM_146146) Mouse Tagged ORF Clone
$1,113.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Lepr |
| Synonyms | db; diabetes; Leprb; LEPROT; Modb1; OB-RGRP; obese-like; obl; Obr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_146146 |
| ORF Size | 3486 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_146146.3 |
| RefSeq Size | 4126 bp |
| RefSeq ORF | 3489 bp |
| Locus ID | 16847 |
| UniProt ID | P48356 |
| Cytogenetics | 4 46.96 cM |
| MW | 131.2 kDa |
| Summary | Receptor for hormone LEP/leptin (Probable) (PubMed:11861497). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS (PubMed:10799542, PubMed:25383904, PubMed:11923481, PubMed:11861497). In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed:10660043, PubMed:12594516). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25383904, PubMed:11923481). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis (PubMed:12594516). Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus (PubMed:25383904). Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (PubMed:9732873).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_146146" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223793 | Lepr (untagged) - Mouse leptin receptor (Lepr), transcript variant 1, (10ug) | 10 ug |
$1,518.00
|
|
| MG226855 | Lepr (tGFP-tagged) - Mouse leptin receptor (Lepr) transcript variant 1, (10ug) | 10 ug |
$789.00
$1,313.00
|
|
| MR226855L3 | Lenti ORF clone of Lepr (Myc-DDK-tagged) - Mouse leptin receptor (Lepr), transcript variant 1 | 10 ug |
$1,413.00
|
|
| MR226855L4 | Lenti ORF clone of Lepr (mGFP-tagged) - Mouse leptin receptor (Lepr), transcript variant 1 | 10 ug |
$1,413.00
|
|
Citations
Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop
Huang, Queen, McMurphy et al
Mol Metab (2019) 30, 48-60 DOI: 10.1016/j.molmet.2019.09.008
Huang, Queen, McMurphy et al
Mol Metab (2019) 30, 48-60 DOI: 10.1016/j.molmet.2019.09.008
Cited by 54
AI Summary: In this study, OriGene products, specifically the PTEN shRNA (OriGene, TL501896) and Lepr shRNA (OriGene, TL502086), were used to knock down PTEN and leptin receptor expression, respectively, enabling the authors to investigate the roles of these genes in adipose tissue homeostasis and redistribution.
Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop
Huang, Queen, McMurphy et al
Molecular Metabolism (2019) 30, 48-60 DOI: 10.1016/j.molmet.2019.09.008
Huang, Queen, McMurphy et al
Molecular Metabolism (2019) 30, 48-60 DOI: 10.1016/j.molmet.2019.09.008
AI Summary: OriGene's LepR expression plasmid (Lepr, MR226855) was used in this study to co-transfect HEK293T cells and validate the efficacy of miR-Lepr constructs in knocking down leptin receptor expression prior to in vivo experiments.
Snippet: … To select the most effective microRNA, the miR-Lepr expressing plasmids were co-transfected with a LepR expression plasmid (Lepr, MR226855, ORIGENE) to HEK293T cells. Western blotting showed the miR-Lepr with targeting sequence of 5′-TATCGTTGCTGACCAGAGTTG- 3′ knocked down the Lepr expression approximately 50% in culture (data not show;... … To select the most effective microRNA, the miR-Lepr expressing plasmids were co-transfected with a LepR expression plasmid (Lepr, MR226855, ORIGENE) to HEK293T cells. Western blotting showed the miR-Lepr with targeting sequence of 5′-TATCGTTGCTGACCAGAGTTG- 3′ knocked down the Lepr expression approximately 50% in culture (data not show;;, the miR-Lepr expressing plasmids were co-transfected with a LepR expression plasmid (Lepr, MR226855, ORIGENE) to HEK293T cells. Western blotting showed the miR-Lepr with targeting sequence of 5′-TATCGTTGCTGACCAGAGTTG- 3′ knocked down the Lepr expression approximately 50% in culture (data not shown). This miR-Lepr was selected for in vivo study. …
Showing 1-2 of 2 papers.
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