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Sall4 (NM_201396) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226829
Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant c
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sall4
Synonyms 5730441M18Rik; AA407717; AL022809; AW536104; C78083; C78563; C330011P20Rik; Tex20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226829 representing NM_201396
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGGCGCAAGCAGGCGAAGCCCCAGCACATCAACTGGGAGGAGGGCCAGGGCGAGCAGCCTCAGC
AGCTACCGAGCCCCGACCTCGCCGAGGCGCTGGCGGCGGAGGAACCCGGTCGTACCAGCCTCCCTCCAAC
ATTTATCCGAGCACAGCCCACCTTTGTCAAAGTTGAAGTGCCTGGCACCTTTGTGGGACCCCCCAGCATG
CCCTCGGGTATGCCGCCTTTGCTAGCATCGCAGCCGCAGCCACGCCGCCAGGCCAAGCAGCACTGCTGCA
CACGGTGTGGAAAGAACTTCTCGTCTGCCAGTGCCCTGCAGATCCACGAGCGAACACACACGGGAGAGAA
GCCTTTCGTGTGTAACATATGCGGGCGGGCCTTCACCACGAAAGGCAACCTGAAGGTACACTACATGACT
CATGGGGCCAACAATAACTCCGCCCGCCGGGGAAGGAAGCTGGCCATAGAGAACCCCATGGCCGCGCTGA
GTGCTGAGGGAAAGAGAGCGCCCGAGGTGTTTTCCAAGGAGCTCCTGTCCCCCGCGGTGAGTGTGGACCC
CGCCTCCTGGAACCAGTACACCAGCGTCCTGAATGGGGGTCTGGCCATGAAGACCAACGAGATCTCCGTG
ATCCAGAGCGGAGGCATCCCCACGCTGCCTGTGTCGCTGGGGGCCAGCTCTGTGGTGAGCAATGGCACGA
TTTCCAAGCTTGACGGCTCTCAGACCGGTGTGAGCATGCCCATGAGCGGGAACGGAGAAAAGCTCGCTGT
TCCCGACGGCATGGCCAAACACCAGTTCCCTCACTTCCTGGAGGAAAATAAGATTGCTGTCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226829 representing NM_201396
Red=Cloning site Green=Tags(s)

MSRRKQAKPQHINWEEGQGEQPQQLPSPDLAEALAAEEPGRTSLPPTFIRAQPTFVKVEVPGTFVGPPSM
PSGMPPLLASQPQPRRQAKQHCCTRCGKNFSSASALQIHERTHTGEKPFVCNICGRAFTTKGNLKVHYMT
HGANNNSARRGRKLAIENPMAALSAEGKRAPEVFSKELLSPAVSVDPASWNQYTSVLNGGLAMKTNEISV
IQSGGIPTLPVSLGASSVVSNGTISKLDGSQTGVSMPMSGNGEKLAVPDGMAKHQFPHFLEENKIAVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201396
ORF Size 834 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201396.3, NP_958798.2
RefSeq Size 2706 bp
RefSeq ORF 837 bp
Locus ID 99377
UniProt ID Q8BX22
Cytogenetics 2 88.99 cM
MW 30.2 kDa
Summary This gene belongs to the spalt family of zinc finger transcription factors. In mouse, functions for this gene have been described in many embryonic developmental processes, including brain, heart, and limb development. In addition, this gene is an important pluripotency factor that is required for stem cell maintenance. Homozygous mutant mice display embryonic lethality, while conditional knock-out in embryonic germ cells results in failure to establish a robust stem cell population. A pseudogene of this gene is found on chromosome 2. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015
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"NM_201396" in other vectors (4)
SKU Description Size Price
MC212042 Sall4 (untagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant c, (10ug) 10 ug
$330.00
MG226829 Sall4 (tGFP-tagged) - Mouse sal-like 4 (Drosophila) (Sall4) transcript variant c, (10ug) 10 ug
$500.00
MR226829L3 Lenti ORF clone of Sall4 (Myc-DDK-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant c 10 ug
$600.00
MR226829L4 Lenti ORF clone of Sall4 (mGFP-tagged) - Mouse sal-like 4 (Drosophila) (Sall4), transcript variant c 10 ug
$600.00

Plasmid Map for MR226829

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226829
Circular map for MR226829

file-alt Citations

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