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Ar (NM_013476) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226737
Ar (Myc-DDK-tagged) - Mouse androgen receptor (Ar)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $823.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ar
Synonyms AW320017; Tfm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226737 representing NM_013476
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGCAGTTAGGGCTGGGAAGGGTCTACCCACGGCCCCCATCCAAGACCTATCGAGGAGCGTTCC
AGAATCTGTTCCAGAGCGTGCGCGAAGCGATCCAGAACCCGGGCCCCAGGCACCCTGAGGCCGCTAACAT
AGCACCTCCCGGCGCCTGTTTACAGCAGAGGCAGGAGACTAGCCCCCGGCGGCGGCGGCGGCAGCAGCAC
ACTGAGGATGGTTCTCCTCAAGCCCACATCAGAGGCCCCACAGGCTACCTGGCCCTGGAGGAGGAACAGC
AGCCTTCACAGCAGCAGGCAGCCTCCGAGGGCCACCCTGAGAGCAGCTGCCTCCCCGAGCCTGGGGCGGC
CACCGCTCCTGGCAAGGGGCTGCCGCAGCAGCCACCAGCTCCTCCAGATCAGGATGACTCAGCTGCCCCA
TCCACGTTGTCCCTGCTGGGCCCCACTTTCCCAGGCTTAAGCAGCTGCTCCGCCGACATTAAAGACATTT
TGAACGAGGCCGGCACCATGCAACTTCTTCAGCAGCAGCAACAACAGCAGCAGCACCAACAGCAGCACCA
ACAGCACCAACAGCAGCAGGAGGTAATCTCCGAAGGCAGCAGCGCAAGAGCCAGGGAGGCCACGGGGGCT
CCCTCTTCCTCCAAGGATAGTTACCTAGGGGGCAATTCAACCATATCTGACAGTGCCAAGGAGTTGTGTA
AAGCAGTGTCTGTGTCCATGGGATTGGGTGTGGAAGCATTGGAACATCTGAGTCCAGGGGAACAGCTTCG
GGGAGACTGCATGTACGCGTCGCTCCTGGGAGGTCCACCCGCGGTGCGTCCCACTCCTTGTGCGCCGCTG
CCCGAATGCAAAGGTCTTCCCCTGGACGAAGGCCCAGGCAAAAGCACTGAAGAGACTGCTGAGTATTCCT
CTTTCAAGGGAGGTTACGCCAAAGGATTGGAAGGTGAGAGCTTGGGGTGCTCTGGCAGCAGTGAAGCAGG
TAGCTCTGGGACACTTGAGATCCCGTCCTCTCTGTCTCTGTATAAATCTGGAGCACTAGACGAGGCAGCA
GCATACCAGAATCGCGACTACTACAACTTTCCGCTGGCTCTGTCCGGGCCGCCGCACCCCCCGCCCCCTA
CCCATCCACACGCCCGTATCAAGCTGGAGAACCCATTGGACTACGGCAGCGCCTGGGCTGCGGCGGCAGC
GCAATGCCGCTATGGGGACTTGGGTAGTCTACATGGAGGGAGTGTAGCCGGGCCCAGCACTGGATCGCCC
CCAGCCACCACCTCTTCTTCCTGGCATACTCTCTTCACAGCTGAAGAAGGCCAATTATATGGGCCAGGAG
GCGGGGGCGGCAGCAGCAGCCCAAGCGATGCCGGGCCTGTAGCCCCCTATGGCTACACTCGGCCCCCTCA
GGGGCTGACAAGCCAGGAGAGTGACTACTCTGCCTCCGAAGTGTGGTATCCTGGTGGAGTTGTGAACAGA
GTACCCTATCCCAGTCCCAATTGTGTCAAAAGTGAAATGGGACCTTGGATGGAGAACTACTCCGGACCTT
ATGGGGACATGCGTTTGGACAGTACCAGGGACCATGTTTTACCCATCGACTATTACTTTCCACCCCAGAA
GACCTGCCTGATCTGTGGAGATGAAGCTTCTGGCTGTCACTACGGAGCTCTCACTTGTGGCAGCTGCAAG
GTCTTCTTCAAAAGAGCCGCTGAAGGGAAACAGAAGTATCTATGTGCCAGCAGAAACGATTGTACCATTG
ATAAATTTCGGAGGAAAAATTGCCCATCTTGTCGTCTCCGGAAATGTTATGAAGCAGGGATGACTCTGGG
AGCTCGTAAGCTGAAGAAACTTGGAAATCTAAAACTACAGGAGGAAGGAGAAAACTCCAATGCTGGCAGC
CCCACTGAGGACCCATCCCAGAAGATGACTGTATCACACATTGAAGGCTATGAATGTCAGCCTATCTTTC
TTAACGTCCTGGAAGCCATTGAGCCAGGAGTGGTGTGTGCCGGACATGACAACAACCAACCAGATTCCTT
TGCTGCCTTGTTATCTAGCCTCAATGAGCTTGGAGAGAGGCAGCTTGTGCATGTGGTCAAGTGGGCCAAG
GCCTTGCCTGGCTTCCGCAACTTGCATGTGGATGACCAGATGGCGGTCATTCAGTATTCCTGGATGGGAC
TGATGGTATTTGCCATGGGTTGGCGGTCCTTCACTAATGTCAACTCCAGGATGCTCTACTTTGCACCTGA
CTTGGTTTTCAATGAGTACCGCATGCACAAGTCTCGGATGTACAGCCAGTGTGTGAGGATGAGGCACCTG
TCTCAAGAGTTTGGATGGCTCCAAATAACCCCCCAGGAATTCCTGTGCATGAAAGCACTGCTGCTCTTCA
GCATTATTCCAGTGGATGGGCTGAAAAATCAAAAATTCTTTGATGAACTTCGAATGAACTACATCAAGGA
ACTCGATCGCATCATTGCATGCAAAAGAAAGAATCCCACATCCTGCTCAAGGCGCTTCTACCAGCTCACC
AAGCTCCTGGATTCTGTGCAGCCTATTGCAAGAGAGCTGCATCAGTTCACTTTTGACCTGCTAATCAAGT
CCCATATGGTGAGCGTGGACTTTCCTGAAATGATGGCAGAGATCATCTCTGTGCAAGTGCCCAAGATCCT
TTCTGGGAAAGTCAAGCCCATCTATTTCCACACACAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226737 representing NM_013476
Red=Cloning site Green=Tags(s)

MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAANIAPPGACLQQRQETSPRRRRRQQH
TEDGSPQAHIRGPTGYLALEEEQQPSQQQAASEGHPESSCLPEPGAATAPGKGLPQQPPAPPDQDDSAAP
STLSLLGPTFPGLSSCSADIKDILNEAGTMQLLQQQQQQQQHQQQHQQHQQQQEVISEGSSARAREATGA
PSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTPCAPL
PECKGLPLDEGPGKSTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGALDEAA
AYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPLDYGSAWAAAAAQCRYGDLGSLHGGSVAGPSTGSP
PATTSSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLTSQESDYSASEVWYPGGVVNR
VPYPSPNCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCK
VFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGENSNAGS
PTEDPSQKMTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAK
ALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHL
SQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLT
KLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_013476
ORF Size 2697 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013476.4
RefSeq Size 2999 bp
RefSeq ORF 2700 bp
Locus ID 11835
UniProt ID P19091
Cytogenetics X 42.82 cM
MW 98.6 kDa
Summary This gene encodes a nuclear hormone receptor containing zinc finger and DNA-binding domains. The encoded protein is a key regulator of signalling by androgens, a class of steroid hormones involved in male reproductive development. The protein responds to hormone signalling by translocating to the nucleus, forming dimers, and binding to androgen response elements (AREs) in the promoters of target genes, which are subsequently transcriptionally activated. Activity of this protein is negatively regulated by nuclear receptor subfamily 0 group B member 1 (Nr0b1, also known as Dax1). Mutations in this gene result in feminized genitals and infertility in male animals. Loss of function in female animals also causes problems in reproductive development and function. provided by RefSeq, May 2015
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Documents
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"NM_013476" in other vectors (4)
SKU Description Size Price
MC222457 Ar (untagged) - Mouse androgen receptor (Ar), (10ug) 10 ug
$824.00
MG226737 Ar (tGFP-tagged) - Mouse androgen receptor (Ar), (10ug) 10 ug
$1,023.00
MR226737L3 Lenti ORF clone of Ar (Myc-DDK-tagged) - Mouse androgen receptor (Ar) 10 ug
$1,123.00
MR226737L4 Lenti ORF clone of Ar (mGFP-tagged) - Mouse androgen receptor (Ar) 10 ug
$1,123.00

Plasmid Map for MR226737

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226737
Circular map for MR226737

file-alt Citations

Strategy for Tumor Selective Disruption of Androgen Receptor Function in the Spectrum of Prostate Cancer
Rosati, Polin, Ducker et al
Clin. Cancer Res. (2018) 24 (24), 6509-6522 DOI: 10.1158/1078-0432.CCR-18-0982
AI Summary: OriGene’s pCMV expression vector containing the full-length androgen receptor (AR) was used as the source for AR cDNA subcloning in this study, enabling functional analyses of AR in prostate cancer models.
Snippet: The full length AR was subcloned from the pCMV expression vector (Origene) into the pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector (System Biosciences) at NheI (upstream) and BamHI (downstream) sites
Showing 1-1 of 1 papers.
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