Tcf7 (NM_009331) Mouse Tagged ORF Clone
$289.00
$300.00
5 Days*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tcf7 |
| Synonyms | AI465550; TCF-1; Tcf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009331 |
| ORF Size | 909 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009331.1 |
| RefSeq Size | 2238 bp |
| RefSeq ORF | 912 bp |
| Locus ID | 21414 |
| UniProt ID | Q00417, Q80UF1, Q00417-2 |
| Cytogenetics | 11 31.86 cM |
| MW | 33.6 kDa |
| Summary | This gene encodes a transcription factor which is a member of the T-cell specific transcription factor family. The encoded protein is distinct from the hepatic transcription factor, transcription factor 1, which is also referred to by the symbol Tcf1. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009331" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209498 | Tcf7 (untagged) - Mouse transcription factor 7, T-cell specific (Tcf7), (10ug) | 10 ug |
$450.00
|
|
| MG226713 | Tcf7 (tGFP-tagged) - Mouse transcription factor 7 T-cell specific (Tcf7), (10ug) | 10 ug |
$500.00
|
|
| MR226713L1 | Lenti ORF clone of Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) | 10 ug |
$600.00
|
|
| MR226713L2 | Lenti ORF clone of Tcf7 (mGFP-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) | 10 ug |
$600.00
|
|
| MR226713L3 | Lenti ORF clone of Tcf7 (Myc-DDK-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) | 10 ug |
$600.00
|
|
| MR226713L4 | Lenti ORF clone of Tcf7 (mGFP-tagged) - Mouse transcription factor 7, T-cell specific (Tcf7) | 10 ug |
$600.00
|
|
Citations
Developmental Relationships of Four Exhausted CD8+ T Cell Subsets Reveals Underlying Transcriptional and Epigenetic Landscape Control Mechanisms
Beltra, Manne, Abdel-Hakeem et al
Immunity (2020) 52 (5), 825-841.e8 DOI: 10.1016/j.immuni.2020.04.014
Beltra, Manne, Abdel-Hakeem et al
Immunity (2020) 52 (5), 825-841.e8 DOI: 10.1016/j.immuni.2020.04.014
Cited by 1115
AI Summary: In this study, the OriGene TCF-1 p33 cDNA (Cat#MR226713) overexpression vector was used to investigate the role of TCF1 in the developmental transitions of exhausted CD8+ T cell subsets.
Snippet: TCF-1 p33 cDNA OriGene Cat#MR226713 TCF-1 p33 overexpression vector In this paper N/A T-bet overexpression vector In this paper N/A Empty-VEX retroviral vector Kurachi et al., 2017 N/A
TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision
Chen, Ji, Ngiow et al
Immunity (2019) 51 (5), 840-855.e5 DOI: 10.1016/j.immuni.2019.09.013
Chen, Ji, Ngiow et al
Immunity (2019) 51 (5), 840-855.e5 DOI: 10.1016/j.immuni.2019.09.013
AI Summary: OriGene’s TCF-1 p33 cDNA clone (MR226713) was used as a key reagent to generate TCF-1 expression constructs that enabled the study of TCF-1’s role in CD8 T cell fate decisions in this research.
Snippet: The TCF-1 p33 (MR226713) cDNA clone was obtained from OriGene and the TCF-1 p45 cDNA clone was extended from the TCF-1 p33 cDNA using PCR. TCF-1 p33 or TCF-1 p45 cDNA were cloned into the MSCV-IRES-VEX plasmid. The c-Myb RV was constructed as described (Chen et al., 2017). The T-bet RV was constructed as described (Kao et al., 2011). RVs were produced in 293T cells with MSCV and pCL
Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells
Johnson, Georgakilas, Petrovic et al
Immunity (2018) 48 (2), 243-257.e10 DOI: 10.1016/j.immuni.2018.01.012
Johnson, Georgakilas, Petrovic et al
Immunity (2018) 48 (2), 243-257.e10 DOI: 10.1016/j.immuni.2018.01.012
AI Summary: OriGene’s mouse Tcf7 cDNA (NM_009331) was used in this study to express the short isoform of TCF-1 in fibroblasts, enabling the investigation of TCF-1’s role in establishing T cell-specific chromatin accessibility and gene expression.
Snippet: …Mouse Tcf7 cDNA (NM_009331) of the short isoform of TCF-1 (p33) was obtained from Origene and cloned into MSCV-ccdB-VEX (MSCV-TCF7-VEX) according to Gateway Clonase II instructions (Invitrogen). …
A Novel miR-24-TCF1 Axis in Modulating Effector T Cell Responses
Cho, Wu, Nguyen et al
J. Immunol. (2017)
Cho, Wu, Nguyen et al
J. Immunol. (2017)
AI Summary: In this study, the OriGene product pCMV6-TCF7 was used as a template to PCR amplify the short isoform of TCF1 (S-TCF1), enabling the authors to investigate the role of TCF1 in miR-24-mediated modulation of effector T cell responses.
Snippet: TGAAGGGGTCGTTGAT-39. Retroviral transduction Long TCF1 isoform (L-TCF1) was PCR amplified from mouse CD4+ T cell mRNA, and short TCF1 isoform (S-TCF1) was PCR amplified by using pCMV6-TCF7 (Origene) as a template. L ... All 2 versions Cite SaveSaving
TCF1 links GIPR signaling to the control of beta cell function and survival
Campbell, Ussher, Mulvihill et al
Nat. Med. (2015) 22, 84-90 DOI: 10.1038/nm.3997
Campbell, Ussher, Mulvihill et al
Nat. Med. (2015) 22, 84-90 DOI: 10.1038/nm.3997
Cited by 148
AI Summary: OriGene contributed plasmid constructs (Tcf7 and Pttg1) and siRNAs (Gipr, Glp1r, Tcf7, TCF7) that were used to manipulate gene expression and investigate the roles of these genes in beta cell function and survival in the study.
Snippet: Peptides were reconstituted in phosphate-buffered saline (PBS), aliquoted and stored at –80 °C. [D-Ala2]GIP (GIP) was from Chi Scientific, Ex4 (exendin-4) was from California Peptide Research Inc. Plasmid constructs (Tcf7 and Pttg1) and siRNA (Gipr, Glp1r, Tcf7, TCF7) were from Origene. The GLP-1R antagonist (JANT-4)44 was a generous gift from R. DiMarchi, University of Indiana
Showing 1-5 of 5 papers.
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