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Dact1 (NM_001190466) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR226691L4
Lenti ORF clone of Dact1 (mGFP-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,046.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dact1
Synonyms 4921528D17Rik; AI115603; DAPPER; DAPPER1; Frd1; FRODO; Frodo1; MDpr1; MTNG3; THYEX3
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR226691).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001190466
ORF Size 2445 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001190466.1, NP_001177395.1
RefSeq Size 3761 bp
RefSeq ORF 2448 bp
Locus ID 59036
Cytogenetics 12 C3
Summary Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB (By similarity). Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling (By similarity). May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.UniProtKB/Swiss-Prot Function
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"NM_001190466" in other vectors (4)
SKU Description Size Price
MC221808 Dact1 (untagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1, (10ug) 10 ug
$822.00
MG226691 Dact1 (tGFP-tagged) - Mouse dapper homolog 1 antagonist of beta-catenin (xenopus) (Dact1) transcript variant 1, (10ug) 10 ug
$946.00
MR226691 Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1 10 ug
$589.00 $746.00
MR226691L3 Lenti ORF clone of Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1 10 ug
$1,046.00

Plasmid Map for MR226691L4

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Cloning scheme for MR226691L4
Circular map for MR226691L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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