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Dact1 (NM_001190466) Mouse Untagged Clone

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SKU
MC221808
Dact1 (untagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $822.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dact1
Synonyms 4921528D17Rik; AI115603; DAPPER; DAPPER1; Frd1; FRODO; Frodo1; MDpr1; MTNG3; THYEX3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221808 representing NM_001190466
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCGGACGCAGCGCGCGAGCCGGAGCCGCTGAGCCCCGGCCGGGGCGCGGAGGCCGAGGGGCGCT
GGCGCGAGAGGGGCGAGGCGGACACGGAGCGGCAGCGTACCCGCGAGCGCCAGGAGGCCACGCTGGCGGG
GCTGGCGGAGCTGGGGTACCTGCGGCAACGCCAAGAGCTGCTGGTGCGCGGTGCGCTGCGCTGCTCCGGG
ACCGTGGGGACCGTCGCGCCGCGCTCCGGGGAGCTGCGGGGAGACGCGGCGCAGCGCAGCCGCCTGGAGG
AGAAGTTCCTGGAAGAGAACATCTTGCTGCTGCGAAGGCAGTTGAATTGTTTGAGGAGAAGAGATGCCGG
TTTGTTGAATCAGTTGCAAGAACTTGACAAGCAGATAAGTGACCTGAGACTGGATGTGGAGAAGACATCT
GAAGAGCACCTGGAGACAGACAGCCGGCCTAGCTCAGGGTTTTATGAGCTGAGTGATGGAGCTTCGGGCT
CCCTCTCTAACTCCTCCAACTCCGTGTTCAGTGAGTGTTTGTCCAGTTGCCATTCCAGCACCTGCTTCTG
CAGCCCCTTGGAGGCGGCCTTGACCATCTCAGACGGTTGCCCCAAATCTGCAGATCTCATAGGATGGTTG
GAATGTAAAGGCGGCCCCTGTGAAGACCAGGCCTCAGGGACAGTGTGCAGTTCCCCCTCCACACCACAAT
TTAATTCCCTTGATGTCATTGCAGATGTGAATCCTAAATACCAGTGTGATCTTGTGTCTAAAAACGGGAA
TGACGTATATCGCTACCCCAGTCCACTTCATGCTGTGGCTGTGCAGAGCCCAATGTTTCTCCTTTGTCTG
ACGGGCAACACTCTGAGGGAAGAGGAGGGGCTTGGGAGCCATGCCAGCGACATCTGCATTGGATCTGAAC
TGAACGCCACCAAAACAGACAATTCCCTGCCATCTCCAAGCAGTTTGTGGTCCGCTTCCCATCCTGCATC
CAGTAAGAAAATGGATGGGTATATTTTGAGCCTCGTGCAGAAGAAAACACACCCTGTAAGGACCAATAAA
CCTAGAACCAGTGTGAACGCTGACCCTACCAAGGGCCTTCTGAGGAATGGAAGTGTGTGTGTCAGGGCCC
CTAGTGGCGTCCCACCGGGCAGTAGTGTGAACTTTAAGAATACAAAACAGATGTGTTTGCCCGCTGGGGG
AATAACCTCTTTGGAAAACGGGCCATTCTCCCCTCCTAAGCAGAGGTCCAAAGACTCAAAGACAGACCAG
TTAGAAAGCAAGAGGTTGGCTCTGCCGGAGAGCTGCTCGGCAGGCGCCGCCATGGAACCCCAAAGCAAGC
ATGTGCCCAAAGCCGCCAAGGCAGCCTCTCAAGAGCTCACAAGGTGTCAAGCCGGGCTGGGGGAATCCAT
GAAGGAAAGCAATCAGGCCTCCGCTGTTTCTCCTAAAACAAGTCCTGGCAGAGGCCCTGTCGCCCCCGCA
GAGAGCAAAGCCCTGCAGCTCCCGAAAAAGATGTCGCAGAAGAACAGCCTCCAGGCTGTGCCCGCGCTGG
ACAGGCCGGCCTTGGACTTCAAAAGCGAGGGCTCATCTCAAAGCCTCGAGGAAGGGCATCTGGTGAAAGC
TCAGTTCATTCCGGGGCAGCAGGCGGCCGCCAGGCCTCACCGTGCACACAGGAACCCGGGTGTCGCAAGG
AGCGCCACCTTGAAGGCCCGCGGCCAGGCAGCCATGGAACACGGCCTGCCCACCGTCAGGGAGAAACCGC
GGGCAGCAGGCAAGAAGTGCCGTTTCCCAGACGACTCGGATACAAATAAGAAATTCAGGAAGACCTCCGC
CAAGGGCCGGCGCAGTGGCGGGCTGCAGGACGCTGGCCTTCCCGGTAGGGCCCTGGGCACCGGCGGCCAT
CGGGCGGGTAGCAGGGCGCACGCGCATGGCCGGGAGCCCGTGGTGGCCAAACCGAAGCACAAGCGAACCG
ACTACCGGCGGTGGAAATCGTCAGCCGAGGTCTCCTACGAAGAAGCCCTGCGGAGGGCCCGGAGGGCTCG
CAGGGAGCACGGGGCTGCCTACCGGGTGGCTGTCGCCCTGCCTTACGCCAGCCCCTATGCCTACGTGCCC
AGCGACTCCGAGTACTCGGCGGAGTGCGAGTCGCTCTTCCACTCCACGGTGGTGGACACCAGCGAGGACG
AGCAGAGCAACTACACCACCAACTGCTTCGGCGACAGCGAGTCCAGCGTGAGCGAAGGCGACTTCGTGGG
CGAGAGCACCACCACCAGCGACTCAGAGGAGAGCGGGGGTTTAATCTGGTCCCAGTTTGTCCAGACTCTC
CCGATTCAAACGGTCACGGCCCCAGACCTCCACACCCGTCCCACAAAAACCTTTGTCAAAATCAAGGCTT
CGCACAACCTCAAGAAGAAGATCCTCCGTTTCCGCTCTGGCTCTTTGAAACTGATGACTACCGTTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001190466
Insert Size 2448 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001190466.1, NP_001177395.1
RefSeq Size 3761 bp
RefSeq ORF 2448 bp
Locus ID 59036
Cytogenetics 12 C3
Summary Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB (By similarity). Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling (By similarity). May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001190466" in other vectors (4)
SKU Description Size Price
MG226691 Dact1 (tGFP-tagged) - Mouse dapper homolog 1 antagonist of beta-catenin (xenopus) (Dact1) transcript variant 1, (10ug) 10 ug
$946.00
MR226691 Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1 10 ug
$589.00 $746.00
MR226691L3 Lenti ORF clone of Dact1 (Myc-DDK-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1 10 ug
$1,046.00
MR226691L4 Lenti ORF clone of Dact1 (mGFP-tagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1 10 ug
$1,046.00

file-alt Citations

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