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Zap70 (NM_009539) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226660
Zap70 (Myc-DDK-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $575.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zap70
Synonyms mrtle; mur; S; Srk; ZAP-; ZAP-70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226660 representing NM_009539
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGATCCCGCGGCGCACCTGCCATTCTTCTATGGCAGCATCTCGCGGGCTGAGGCCGAGGAGCACC
TGAAGCTGGCAGGCATGGCCGACGGGCTGTTCCTCCTGCGCCAGTGTTTGCGCTCCCTGGGCGGCTACGT
GCTGTCGTTGGTGCACGACGTGCGCTTCCACCATTTCCCCATCGAGCGCCAACTCAACGGCACGTACGCC
ATCGCGGGCGGGAAGGCGCACTGCGGCCCGGCCGAGCTCTGCCAGTTCTACTCTCAGGACCCCGACGGGC
TGCCCTGCAACCTGCGTAAGCCGTGTAACCGGCCGCCCGGACTGGAGCCACAGCCCGGGGTCTTCGACTG
CCTGCGTGATGCTATGGTGCGCGACTACGTGCGCCAGACCTGGAAGCTGGAGGGCGATGCACTAGAGCAG
GCCATCATCAGCCAGGCCCCACAGGTGGAGAAGCTCATTGCTACCACAGCCCACGAGCGAATGCCCTGGT
ATCACAGCAGCCTGACTCGTGAGGAGGCCGAGCGCAAACTCTATTCCGGCCAGCAGACCGACGGCAAGTT
CCTGCTGAGGCCCCGGAAGGAGCAGGGCACATATGCACTGTCCCTGGTCTATGGGAAAACTGTATACCAC
TATCTCATCAGCCAGGACAAGGCTGGCAAGTACTGCATCCCCGAAGGCACCAAGTTTGACACGCTCTGGC
AGCTGGTGGAGTACCTGAAGCTGAAGGCGGATGGACTAATTTACCGCTTGAAGGAGGTCTGTCCCAACAG
CAGCGCCAGCGCGGCAGTTGCTGCACCCACCCTCCCAGCCCACCCATCCACATTCACTCAGCCTCAGAGA
CGAGTGGACACCCTGAACTCGGACGGATACACCCCTGAACCAGCACGCTTAGCGTCATCGACAGACAAGC
CCCGGCCGATGCCCATGGACACAAGTGTGTACGAGAGTCCCTACAGCGACCCTGAGGAACTCAAAGACAA
GAAGCTCTTCCTGAAGCGAGAGAATCTCCTCGTGGCGGACATCGAGCTTGGCTGTGGCAACTTTGGCTCC
GTGCGCCAGGGAGTCTATCGCATGCGCAAGAAGCAGATTGACGTGGCCATCAAGGTGCTGAAGCAGGGCA
CAGAGAAGGCCGACAAAGATGAGATGATGCGAGAGGCCCAGATCATGCACCAGCTCGACAACCCCTACAT
CGTGCGGCTCATCGGCGTGTGCCAGGCAGAAGCACTCATGCTGGTCATGGAGATGGCGGGAGGCGGGCCC
CTGCACAAGTTCCTGCTGGGAAAGAAGGAGGAGATCCCTGTGAGCAATGTGGCTGAACTGCTGCACCAGG
TGGCCATGGGCATGAAGTATTTGGAGGAGAAAAACTTTGTGCACCGCGACCTGGCAGCCCGCAATGTTCT
ACTGGTCAATCGGCACTATGCCAAGATCAGCGACTTTGGCCTGTCCAAAGCCCTGGGTGCTGACGACAGC
TATTACACAGCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGA
AGTTCTCCAGCCGCAGTGACGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTCTCCTATGGCCAGAA
GCCCTACAAGAAAATGAAGGGCCCCGAGGTCCTGGACTTCATCAAGCAGGGTAAGAGGATGGAATGTCCG
CCGGAGTGTCCTCCTGAGATGTATGCACTTATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCCCCG
ACTTCCTGACTGTGGAACAACGTATGCGGAACTATTACTACAGCCTGGCCAGCCGGGCCGAGGGACCCCC
ACAGTGTGAACAGGTGGCCGAGGCTGCATGTGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226660 representing NM_009539
Red=Cloning site Green=Tags(s)

MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYA
IAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQ
AIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYH
YLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQR
RVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGS
VRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGP
LHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDS
YYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECP
PECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009539
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009539.3, NP_033565.2
RefSeq Size 2180 bp
RefSeq ORF 1857 bp
Locus ID 22637
UniProt ID P43404
Cytogenetics 1 15.41 cM
MW 70.6 kDa
Summary This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2014
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"NM_009539" in other vectors (4)
SKU Description Size Price
MC219715 Zap70 (untagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), (10ug) 10 ug
$634.00
MG226660 Zap70 (tGFP-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), (10ug) 10 ug
$775.00
MR226660L3 Lenti ORF clone of Zap70 (Myc-DDK-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70) 10 ug
$875.00
MR226660L4 Lenti ORF clone of Zap70 (mGFP-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70) 10 ug
$875.00

Plasmid Map for MR226660

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226660
Circular map for MR226660

file-alt Citations

TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells
Zhang, Liu, Yang et al
Mol Cell (2022) 82 (16), 3077-3088.e6 DOI: 10.1016/j.molcel.2022.06.002
Cited by 57
AI Summary: OriGene products pCMV6-Zap70 (MR226660), pCMV6-Lck (MR227319), and pCMV6-Lat (MG202998) were used in this study to investigate the roles of key signaling proteins in TCR-mediated activation and PYGB-dependent glycogenolysis during the early recall response of CD8+ memory T cells.
Snippet: Glycolysis facilitates the rapid recall response of CD8 + memory T (Tm) cells. However, it remains unclear whether Tm cells uptake exogenous glucose or mobilize Save;pCMV6-Zap70 Origene MR226660 pCMV6-Lck Origene MR227319 pCMV6-Lat Origene MG202998
Showing 1-1 of 1 papers.
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