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D1Pas1 (NM_033077) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226486
D1Pas1 (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $614.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D1Pas1
Synonyms Pl10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226486 representing NM_033077
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCATGTGGCAGAGGAAGATGAGCTCGGGCTGGACCAGCAGTTGGCTGGCCTAGACCTGACGTCTC
GGGACAGCCAGAGCGGGGGCAGCACAGCCAGCAAAGGGCGCTACATCCCCCCTCACCTGCGGAACCGGGA
GGCCGCGAAGGCGTTCTACGACAAAGACGGCTCACGGTGGAGTAAAGACAAGGATGCCTACAGCAGCTTC
GGGTCCCGCAGCGACACGAGAGCCAAGTCCAGTTTCTTCAGTGACCGCGGCGGAAGCGGCTCCAGGGGAA
GGTTTGACGAGCGCGGGCGGAGTGACTACGAGAGTGTTGGCAGCCGGGGAGGTAGAAGTGGCTTTGGCAA
ATTTGAGCGGGGCGGGAACAGTCGCTGGTGTGACAAAGCAGACGAGGATGACTGGTCGAAACCGCTCCCA
CCAAGCGAACGCTTGGAACAGGAACTCTTTTCCGGAGGCAACACTGGGATTAACTTTGAGAAATATGATG
ACATCCCGGTTGAGGCGACAGGGAATAACTGCCCTCCACATATTGAAAGTTTCAGCGATGTCGAGATGGG
AGAGATTATTATGGGAAACATCGAGCTCACTCGTTACACACGCCCAACTCCTGTGCAAAAGCATGCTATT
CCTATTATCAAGGAGAAAAGAGACTTGATGGCTTGTGCCCAAACGGGGTCTGGAAAAACAGCAGCTTTTC
TTCTACCTATCTTGAGTCAGATCTATACGGATGGTCCAGGTGAGGCTTTGAGGGCCATGAAGGAGAATGG
AAAGTATGGGCGTCGCAAACAGTATCCAATCTCCTTGGTCTTGGCTCCGACCAGAGAATTGGCCGTGCAG
ATCTATGAGGAGGCCAGGAAATTCTCATACCGCTCTCGAGTTCGTCCTTGTGTGGTTTATGGTGGAGCTG
ATATTGGGCAGCAGATTCGAGACTTGGAACGTGGCTGTCACTTGTTAGTAGCTACTCCAGGACGGCTAGT
AGATATGATGGAGAGAGGAAAGATTGGACTGGACTTCTGCAAATACTTGGTGTTAGATGAAGCAGATCGA
ATGTTGGACATGGGCTTTGAGCCTCAGATACGAAGAATAGTTGAGCAAGACACCATGCCTCCAAAAGGTG
TCCGCCACACTATGATGTTTAGTGCTACCTTTCCTAAGGAAATACAGATGCTGGCTCGGGATTTCTTGGA
TGAATATATCTTTCTGGCCGTAGGAAGAGTTGGGTCTACTTCTGAGAACATCACACAGAAAGTCGTGTGG
GTGGAAGAAGCAGACAAGCGGTCATTTCTGCTTGACCTCCTGAATGCAACGGGCAAGGATTCCCTGATAT
TGGTGTTTGTGGAAACCAAAAAGGGTGCAGATTCTCTGGAGGACTTCTTATACCACGAAGGATATGCTTG
TACCAGTATCCATGGAGACCGATCTCAGCGAGATAGGGAAGAGGCCCTTCACCAATTCCGATCAGGAAAA
AGCCCAATTCTCGTGGCTACAGCAGTAGCTGCAAGAGGACTGGATATTTCCAATGTGAAACATGTCATCA
ATTTCGACTTGCCTAGTGACATCGAAGAATATGTGCATCGCATTGGCCGTACAGGTCGTGTTGGAAACCT
TGGCCTTGCCACCTCATTCTTCAACGAGAGGAACATAAATATTACCAAAGATTTATTGGATCTTCTTGTT
GAAGCAAAACAAGAAGTGCCATCTTGGCTAGAAAACATGGCTTTTGAACACCATTACAAGGGTGGCAGTC
GTGGACGGTCCAAGAGCAGATTTAGTGGAGGATTTGGTGCTAGAGACTATCGACAAAGTAGCGGTGCCAG
CAGTTCCAGCTTCAGCAGTGGCCGTGCTAGCAACAGTCGCAGCGGTGGAGGCAGCCATGGCAGCAGCAGA
GGGTTTGGTGGAGGTAGCTATGGAGGCTTTTACAACAGTGATGGATATGGAGGAAATTATAGCTCCCAGG
GGGTTGACTGGTGGGGTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226486 representing NM_033077
Red=Cloning site Green=Tags(s)

MSHVAEEDELGLDQQLAGLDLTSRDSQSGGSTASKGRYIPPHLRNREAAKAFYDKDGSRWSKDKDAYSSF
GSRSDTRAKSSFFSDRGGSGSRGRFDERGRSDYESVGSRGGRSGFGKFERGGNSRWCDKADEDDWSKPLP
PSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAI
PIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALRAMKENGKYGRRKQYPISLVLAPTRELAVQ
IYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADR
MLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVW
VEEADKRSFLLDLLNATGKDSLILVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGK
SPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLV
EAKQEVPSWLENMAFEHHYKGGSRGRSKSRFSGGFGARDYRQSSGASSSSFSSGRASNSRSGGGSHGSSR
GFGGGSYGGFYNSDGYGGNYSSQGVDWWGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033077
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033077.3, NP_149068.1
RefSeq Size 3212 bp
RefSeq ORF 1983 bp
Locus ID 110957
UniProt ID P16381
Cytogenetics 1 90.09 cM
MW 73.6 kDa
Summary Putative ATP-dependent RNA helicase. Possible role in a key step of the spermatogenic process.UniProtKB/Swiss-Prot Function
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"NM_033077" in other vectors (4)
SKU Description Size Price
MC220194 D1Pas1 (untagged) - Mouse DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1), (10ug) 10 ug
$677.00
MG226486 D1Pas1 (tGFP-tagged) - Mouse DNA segment Chr 1 Pasteur Institute 1 (D1Pas1), (10ug) 10 ug
$814.00
MR226486L3 Lenti ORF clone of D1Pas1 (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1) 10 ug
$914.00
MR226486L4 Lenti ORF clone of D1Pas1 (mGFP-tagged) - Mouse DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1) 10 ug
$914.00

Plasmid Map for MR226486

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226486
Circular map for MR226486

file-alt Citations

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