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Lpl (NM_008509) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226332
Lpl (Myc-DDK-tagged) - Mouse lipoprotein lipase (Lpl)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lpl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226332 representing NM_008509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGCAAAGCCCTGCTCCTGGTGGTCCTGGGAGTTTGGCTCCAGAGTTTGACCGCCTTCCGAGGAG
GGGTGGCCGCAGCAGACGCGGGAAGAGATTTCTCAGACATCGAAAGCAAATTTGCCCTAAGGACCCCTGA
AGACACAGCTGAGGACACTTGTCATCTCATTCCTGGATTAGCAGACTCTGTGTCTAACTGCCACTTCAAC
CACAGCAGCAAGACCTTCGTGGTGATCCATGGATGGACGGTAACGGGAATGTATGAGAGTTGGGTGCCCA
AACTTGTGGCCGCCCTGTACAAGAGAGAACCTGACTCCAATGTCATTGTAGTAGACTGGTTGTATCGGGC
CCAGCAACATTATCCAGTGTCAGCTGGCTACACCAAGCTGGTGGGAAATGATGTGGCCAGATTCATCAAC
TGGATGGAGGAGGAGTTTAACTACCCCCTAGACAACGTCCACCTCTTAGGGTACAGCCTTGGAGCCCATG
CTGCTGGCGTAGCAGGAAGTCTGACCAATAAGAAGGTCAATAGAATTACTGGTTTGGATCCAGCTGGGCC
TAACTTTGAGTATGCAGAAGCCCCCAGTCGCCTTTCTCCTGATGACGCTGATTTTGTAGATGTCTTACAC
ACATTTACCAGGGGGTCACCTGGTCGAAGTATTGGAATCCAGAAACCAGTAGGGCATGTTGACATTTATC
CCAATGGAGGCACTTTCCAGCCAGGATGCAACATTGGAGAAGCCATCCGTGTGATTGCAGAGAGAGGACT
CGGAGACGTGGACCAGCTGGTGAAGTGCTCGCACGAGCGCTCCATTCATCTCTTCATTGACTCCCTGCTG
AATGAAGAAAACCCCAGCAAGGCATACAGGTGCAACTCCAAGGAAGCCTTTGAGAAAGGGCTCTGCCTGA
GTTGTAGAAAGAATCGCTGTAACAATCTGGGCTATGAGATCAACAAGGTCAGAGCCAAGAGAAGCAGCAA
GATGTACCTGAAGACTCGCTCTCAGATGCCCTACAAAGTGTTCCATTACCAAGTCAAGATTCACTTTTCT
GGGACTGAGGATGGCAAGCAACACAACCAGGCCTTCGAGATTTCTCTGTACGGCACAGTGGCCGAGAGCG
AGAACATTCCCTTCACCCTGCCCGAGGTTTCCACAAATAAAACCTACTCCTTCTTGATTTACACGGAGGT
GGACATCGGAGAACTGCTCATGATGAAGCTTAAGTGGATAAGCGACTCCTACTTCAGCTGGCCTGACTGG
TGGAGCAGCCCCAGCTTCGTCATCGAGAGGATCCGAGTGAAAGCCGGAGAGACTCAGAAAAAGGTCATCT
TCTGTGCTAGGGAGAAAGTTTCTCATCTGCAGAAGGGAAAGGACTCAGCAGTGTTTGTGAAATGCCATGA
CAAGTCTCTGAAGAAGTCTGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226332 representing NM_008509
Red=Cloning site Green=Tags(s)

MESKALLLVVLGVWLQSLTAFRGGVAAADAGRDFSDIESKFALRTPEDTAEDTCHLIPGLADSVSNCHFN
HSSKTFVVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLYRAQQHYPVSAGYTKLVGNDVARFIN
WMEEEFNYPLDNVHLLGYSLGAHAAGVAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLH
TFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLL
NEENPSKAYRCNSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFS
GTEDGKQHNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMMKLKWISDSYFSWPDW
WSSPSFVIERIRVKAGETQKKVIFCAREKVSHLQKGKDSAVFVKCHDKSLKKSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008509
ORF Size 1422 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008509.2, NP_032535.2
RefSeq Size 4049 bp
RefSeq ORF 1425 bp
Locus ID 16956
UniProt ID P11152
Cytogenetics 8 33.88 cM
MW 53.6 kDa
Summary Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed:8675619). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed:24726386). Recruited to its site of action on vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed:20620994, PubMed:24726386, PubMed:27811232).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008509" in other vectors (4)
SKU Description Size Price
MC216467 Lpl (untagged) - Mouse lipoprotein lipase (Lpl), (10ug) 10 ug
$732.00
MG226332 Lpl (tGFP-tagged) - Mouse lipoprotein lipase (Lpl), (10ug) 10 ug
$886.00
MR226332L3 Lenti ORF clone of Lpl (Myc-DDK-tagged) - Mouse lipoprotein lipase (Lpl) 10 ug
$986.00
MR226332L4 Lenti ORF clone of Lpl (mGFP-tagged) - Mouse lipoprotein lipase (Lpl) 10 ug
$986.00

Plasmid Map for MR226332

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226332
Circular map for MR226332

file-alt Citations

ULK1 prevents cardiac dysfunction in obesity through autophagy-meditated regulation of lipid metabolism
An, Ryu, Won Park et al
Cardiovasc. Res. (2017)
AI Summary: OriGene's LPL cDNA clone (NM_008509) was used to produce a ^32P-labeled oligonucleotide probe, enabling the authors to study LPL expression in their investigation of autophagy and cardiac lipid metabolism.
Snippet: A 32 P-labeled oligonucleotide probe was produced using LPL cDNA clone (NM_008509;OriGene Technologies, Inc)
Showing 1-1 of 1 papers.
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