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Clock (NM_007715) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR226315L4
Lenti ORF clone of Clock (mGFP-tagged) - Mouse circadian locomotor output cycles kaput (Clock)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,083.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Clock or NM_007715"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clock
Synonyms 5330400M04Rik; KAT13D
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR226315).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007715
ORF Size 2565 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007715.5
RefSeq Size 7478 bp
RefSeq ORF 2568 bp
Locus ID 12753
UniProt ID O08785
Cytogenetics 5 40.63 cM
Summary The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding the same protein have been found for this gene. provided by RefSeq, Jan 2014
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"NM_007715" in other vectors (4)
SKU Description Size Price
MC222139 Clock (untagged) - Mouse circadian locomotor output cycles kaput (Clock), (10ug) 10 ug
$784.00
MG226315 Clock (tGFP-tagged) - Mouse circadian locomotor output cycles kaput (Clock), (10ug) 10 ug
$789.00 $983.00
MR226315 Clock (Myc-DDK-tagged) - Mouse circadian locomotor output cycles kaput (Clock) 10 ug
$589.00 $783.00
MR226315L3 Lenti ORF clone of Clock (Myc-DDK-tagged) - Mouse circadian locomotor output cycles kaput (Clock) 10 ug
$1,083.00

Plasmid Map for MR226315L4

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR226315L4
Circular map for MR226315L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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