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Neo1 (NM_008684) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226235
Neo1 (Myc-DDK-tagged) - Mouse neogenin (Neo1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,276.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Neo1
Synonyms 2610028H22Rik; AI327052; D930014N22Rik; Igdcc2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226235 representing NM_008684
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGAGCGCGAAGCCGGGCGACTCCTCTGCACCTCCTCCTCCCGGCGCTGCTGTCCGCCACCGC
CGCTGCTGCTGTTGCTGCCGCTGCTGCTGCTGCTCGGACGCCCGGCGTCCGGCGCCGCGGCCACGAAGAG
CGGCTCCCCGCCGCAGTCCGCAGGAGCCAGTGTTCGAACATTCACTCCGTTTTATTTTCTGGTGGAGCCA
GTAGACACCCTCTCAGTTAGAGGCTCTTCTGTTATATTAAATTGCTCGGCATATTCTGAGCCCTCTCCAA
ACATTGAATGGAAGAAAGATGGGACTTTTTTAAACTTAGAATCAGATGATCGACGCCAGCTACTCCCAGA
TGGATCTTTATTCATCAGCAACGTGGTGCATTCCAAACACAATAAGCCTGACGAAGGTTTCTATCAGTGT
GTAGCCACTGTGGATAATCTTGGAACCATTGTCAGCAGAACAGCCAAGCTCACAGTAGCAGGTCTTCCAA
GATTTACCAGCCAACCAGAACCTTCTTCAGTCTATGTTGGAAACAGTGCAATTCTGAATTGTGAAGTTAA
TGCAGATTTGGTCCCATTTGTTAGGTGGGAACAGAATCGACAGCCCCTTCTTCTAGATGACAGGATTGTC
AAACTTCCAAGTGGAACACTGGTTATCAGCAATGCTACTGAAGGAGATGGGGGACTCTACCGCTGCATTG
TTGAAAGTGGTGGGCCACCAAAGTTTAGTGACGAAGCTGAATTGAAAGTTCTTCAAGATCCTGAGGAAAT
TGTAGACTTGGTATTTCTGATGCGACCATCTTCTATGATGAAAGTCACTGGTCAGAGTGCAGTGTTGCCA
TGTGTTGTCTCAGGGCTTCCTGCTCCAGTTGTTAGATGGATGAAAAACGAAGAAGTGCTTGACACAGAAA
GCTCTGGCAGGTTGGTCTTGCTAGCAGGAGGTTGCTTGGAGATCAGTGATGTCACTGAGGATGATGCTGG
GACTTATTTTTGCATAGCTGATAATGGAAATAAGACAGTTGAAGCTCAGGCGGAGCTTACTGTGCAAGTG
CCACCTGGATTCCTGAAACAACCTGCTAACATATATGCTCACGAATCCATGGACATTGTATTTGAATGTG
AAGTCACTGGGAAGCCAACTCCAACTGTGAAGTGGGTCAAGAATGGGGATGTGGTTATCCCCAGTGATTA
CTTTAAAATTGTAAAGGAACATAATCTTCAAGTTTTGGGTCTGGTGAAATCAGATGAAGGGTTCTATCAA
TGCATTGCTGAGAATGATGTTGGAAATGCACAAGCTGGAGCCCAGCTGATAATCCTTGAGCATGATGTTG
CCATCCCAACATTACCTCCCACTTCACTGACCAGTGCCACTACTGACCATCTAGCACCAGCCACAACGGG
ACCATTACCTTCAGCTCCTCGAGACGTCGTGGCCTCCCTGGTCTCTACTCGCTTCATTAAATTGACATGG
CGTACACCTGCATCAGACCCTCATGGAGACAATCTCACCTACTCTGTGTTCTACACCAAGGAAGGGGTTG
CTAGGGAGCGTGTTGAGAATACCAGCCAGCCAGGAGAGATGCAGGTGACTATTCAAAACTTGATGCCAGC
AACTGTGTACATCTTCAAAGTTATGGCTCAAAATAAGCATGGCTCTGGAGAAAGTTCAGCTCCTCTTCGA
GTAGAGACACAGCCTGAGGTTCAGCTCCCTGGCCCAGCACCTAATATCCGTGCTTATGCAACGTCACCTA
CTTCTATCACTGTCACCTGGGAAACACCGTTATCTGGCAATGGGGAAATTCAAAATTACAAATTGTACTA
CATGGAAAAAGGAACTGATAAAGAACAGGATATTGATGTTTCAAGTCACTCCTACACCATTAATGGACTG
AAGAAATACACAGAATACAGTTTCCGAGTGGTGGCCTACAATAAACATGGTCCTGGAGTTTCTACACAAG
ATGTTGCTGTTCGAACATTATCAGATGTTCCCAGTGCTGCTCCTCAGAATCTGTCCTTAGAAGTGAGAAA
TTCAAAGAGTATAGTGATCCACTGGCAGCCCCCTTCCTCAACCACACAAAATGGGCAGATAACTGGCTAC
AAGATTCGATATCGAAAGGCCTCCCGAAAAAGTGATGTCACTGAGACCTTGGTAACTGGGACACAGCTGT
CTCAGCTGATTGAAGGTCTTGATCGGGGGACAGAATATAACTTCCGAGTCGCTGCTCTCACAGTCAATGG
TACAGGTCCAGCAACTGATTGGCTGTCTGCTGAAACTTTTGAAAGCGACCTAGATGAAACTCGTGTTCCT
GAAGTGCCCAGCTCTCTTCATGTCCGTCCGCTCGTCACTAGCATTGTAGTGAGCTGGACTCCTCCAGAGA
ACCAGAACATTGTGGTCCGAGGTTATGCCATCGGTTACGGCATTGGCAGCCCTCATGCCCAGACCATCAA
AGTGGACTATAAACAACGTTATTACACCATCGAAAACTTGGATCCAAGCTCTCATTACGTGATTACCTTG
AAAGCATTTAACAATGTTGGCGAAGGCATCCCCCTTTATGAGAGTGCTGTGACCAGACCTCACACAGACA
CTTCTGAAGTTGATTTATTTGTTATTAATGCTCCATACACTCCAGTGCCAGATCCCACTCCCATGATGCC
ACCAGTGGGAGTTCAGGCTTCCATTCTGAGTCACGACACCATAAGGATTACCTGGGCAGACAACTCCCTG
CCCAAACACCAGAAGATTACAGACTCCCGCTACTACACAGTCCGGTGGAAGACCAACATCCCAGCAAACA
CGAAGTACAAGAATGCAAATGCAACGACGTTAAGCTATTTGGTTACTGGTTTAAAGCCAAATACGCTCTA
TGAGTTCTCTGTGATGGTGACCAAAGGCAGAAGGTCAAGCACGTGGAGTATGACAGCTCATGGCGCTACC
TTTGAATTAGTTCCTACTTCTCCACCTAAGGATGTGACAGTTGTGAGTAAGGAAGGAAAACCTAGAACCA
TCATAGTGAATTGGCAGCCTCCCTCTGAAGCTAACGGCAAGATTACAGGTTACATCATCTATTACAGCAC
GGATGTGAATGCAGAGATACATGACTGGGTTATTGAACCAGTTGTGGGAAACAGACTGACTCACCAGATT
CAAGAGTTAACACTTGATACGCCATACTACTTCAAAATCCAGGCCCGGAACTCAAAGGGCATGGGGCCCA
TGTCTGAAGCTGTACAGTTCAGAACACCTAAAGCGGACTCCTCTGATAAAATGCCTAATGACCAAGCCTT
AGGGTCAGCAGGAAAAGGAAGCCGACTACCAGACCTGGGATCTGACTACAAACCTCCAATGAGTGGCAGC
AACAGCCCTCACGGGAGCCCCACCTCCCCTCTGGACAGCAACATGCTGCTGGTCATCATTGTCTCTGTTG
GCGTCATCACTATCGTGGTGGTTGTGGTCATTGCTGTCTTTTGTACCCGGCGCACCACCTCTCACCAGAA
GAAGAAACGAGCTGCGTGCAAATCAGTGAATGGCTCCCATAAGTACAAGGGCAATTGCAAAGATGTGAAG
CCTCCAGACCTATGGATCCATCACGAGAGACTAGAGTTGAAGCCTATTGACAAGTCTCCAGATCCTAACC
CTGTCATGACTGATACTCCAATCCCTCGAAACTCTCAAGATATCACACCAGTGGACAATTCCATGGATAG
CAATATCCATCAAAGGCGGAATTCATACAGAGGGCATGAGTCAGAGGACAGCATGTCTACACTGGCTGGA
AGGAGGGGAATGAGACCAAAAATGATGATGCCCTTTGACTCTCAGCCACCTCAGCCTGTGATTAGTGCCC
ATCCCATCCATTCCCTCGATAACCCTCACCATCATTTCCACTCCAGCAGCCTCGCTTCTCCAGCCCGCAG
TCATCTCTACCACCCAAGCAGCCCATGGCCCATTGGCACATCCATGTCCCTTTCAGACAGGGCCAATTCC
ACAGAATCTGTTCGAAATACCCCCAGCACGGACACCATGCCAGCGTCCTCGTCTCAGACGTGCTGCACTG
ACCATCAGGACCCTGAGGGTGCTACTAGCTCCTCTTACTTGGCCAGCTCCCAAGAGGAAGACTCAGGCCA
GAGTCTTCCCACAGCCCATGTCCGCCCTTCCCACCCTCTGAAGAGCTTCGCTGTGCCAGCAATCCCACCC
CCAGGACCTCCTCTCTATGATCCTGCACTGCCAAGCACACCATTACTGTCCCAGCAAGCTCTGAACCATC
ACATTCACTCAGTGAAAACAGCCTCCATCGGGACGTTAGGAAGGAGCCGGCCTCCTATGCCAGTGGTTGT
TCCGAGTGCCCCTGAAGTACAGGAGACCACCAGGATGCTGGAAGACTCCGAGAGTAGCTATGAACCAGAT
GAGCTGACCAAAGAGATGGCCCACCTGGAAGGACTAATGAAGGACCTAAATGCCATCACAACAGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR226235 representing NM_008684
Red=Cloning site Green=Tags(s)

MAAEREAGRLLCTSSSRRCCPPPPLLLLLPLLLLLGRPASGAAATKSGSPPQSAGASVRTFTPFYFLVEP
VDTLSVRGSSVILNCSAYSEPSPNIEWKKDGTFLNLESDDRRQLLPDGSLFISNVVHSKHNKPDEGFYQC
VATVDNLGTIVSRTAKLTVAGLPRFTSQPEPSSVYVGNSAILNCEVNADLVPFVRWEQNRQPLLLDDRIV
KLPSGTLVISNATEGDGGLYRCIVESGGPPKFSDEAELKVLQDPEEIVDLVFLMRPSSMMKVTGQSAVLP
CVVSGLPAPVVRWMKNEEVLDTESSGRLVLLAGGCLEISDVTEDDAGTYFCIADNGNKTVEAQAELTVQV
PPGFLKQPANIYAHESMDIVFECEVTGKPTPTVKWVKNGDVVIPSDYFKIVKEHNLQVLGLVKSDEGFYQ
CIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATTGPLPSAPRDVVASLVSTRFIKLTW
RTPASDPHGDNLTYSVFYTKEGVARERVENTSQPGEMQVTIQNLMPATVYIFKVMAQNKHGSGESSAPLR
VETQPEVQLPGPAPNIRAYATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDIDVSSHSYTINGL
KKYTEYSFRVVAYNKHGPGVSTQDVAVRTLSDVPSAAPQNLSLEVRNSKSIVIHWQPPSSTTQNGQITGY
KIRYRKASRKSDVTETLVTGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVP
EVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITL
KAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSL
PKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGAT
FELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQI
QELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMPNDQALGSAGKGSRLPDLGSDYKPPMSGS
NSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVVIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNCKDVK
PPDLWIHHERLELKPIDKSPDPNPVMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAG
RRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPSSPWPIGTSMSLSDRANS
TESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPP
PGPPLYDPALPSTPLLSQQALNHHIHSVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPD
ELTKEMAHLEGLMKDLNAITTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008684
ORF Size 4476 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008684.2, NP_032710.2
RefSeq Size 7388 bp
RefSeq ORF 4479 bp
Locus ID 18007
Cytogenetics 9 B
MW 163.4 kDa
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"NM_008684" in other vectors (4)
SKU Description Size Price
MC224553 Neo1 (untagged) - Mouse neogenin (Neo1), transcript variant 1, (10ug) 10 ug
$1,277.00
MG226235 Neo1 (tGFP-tagged) - Mouse neogenin (Neo1) transcript variant 1, (10ug) 10 ug
$789.00 $1,476.00
MR226235L3 Lenti ORF clone of Neo1 (Myc-DDK-tagged) - Mouse neogenin (Neo1), transcript variant 1 10 ug
$1,576.00
MR226235L4 Lenti ORF clone of Neo1 (mGFP-tagged) - Mouse neogenin (Neo1), transcript variant 1 10 ug
$1,576.00

Plasmid Map for MR226235

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226235
Circular map for MR226235

file-alt Citations

The neogenin-hemojuvelin axis in murine liver is crucial for hepcidin expression
Enns, Jue, Bethel et al
American Journal of Physiology-Gastrointestinal and Liver Physiology (2026)
AI Summary: OriGene products contributed to this study by providing murine Neo1 ORF (NM_008684) and murine Dcc ORF (NM_007831) constructs with C-terminal FLAG/MYC tags in pCMV6 vectors (#MR226235 and #MR226661), which were used to investigate the roles of Neo1 and Dcc in hepcidin regulation.
Snippet: Murine Neo1 ORF (NM_008684) and murine deleted in colorectal cancer (Dcc) ORF 151 (NM_007831), each containing a C-terminal FLAG/MYC epitope (fNeo1 and fDcc, respectively), were 152 purchased in pCMV6 vectors (#MR226235 and #MR226661;OriGene Technologies). Sequence integrity 153 was validated by DNA sequencing. The fDcc construct was subcloned into an AAV
Matriptase-2-mediated suppression of hepatic hepcidin expression in mice requires hepatocyte neogenin
Enns, Jue, Zhang
J Biol Chem (2026) 302 (3), 111142 DOI: 10.1016/j.jbc.2026.111142
Cited by 2
AI Summary: OriGene products contributed to this study by providing the murine Neo1 ORF (NM_008684) with a C-terminal FLAG/MYC epitope in pCMV6 vector (#MR226235) and the murine Mt2 ORF (NM_027902.1) with a C-terminal FLAG/MYC epitope in pCMV6 vector (#MR210781), which were used for functional analyses of Neo1 and Mt2 in hepcidin regulation.
Snippet: We purchased murine Neo1 ORF (NM_008684) with a C-terminal FLAG/MYC epitope (fNeo1) in pCMV6 vector (#MR226235) and murine Mt2 ORF (NM_027902.1) with a C-terminal FLAG/MYC epitope (fMt2) in pCMV6 vector (#MR210781) from OriGene Technologies Inc
Hepcidin expression is associated with increased γ-secretase-mediated cleavage of neogenin in the liver
Enns, Zhang, Jue et al
J Biol Chem (2024)
Cited by 3
AI Summary: OriGene products—specifically, the mouse Neo1 ORF with a C-terminal FLAG/MYC epitope in pCMV6 vector (#MR226235) and the mouse Alk3 ORF with a C-terminal FLAG/MYC epitope in pCMV6 vector (#MR227586)—were used to express and study the roles of Neo1 and Alk3 in investigating the γ-secretase-mediated cleavage of Neo1 and its effect on hepcidin expression in the liver.
Snippet: Mouse Neo1 ORF (NM_008684) with a C-terminal FLAG/MYC epitope (fNeo1) in pCMV6 vector (#MR226235) and mouse Alk3 ORF (NM_009758.3) with a C-terminal FLAG/MYC epitope (fAlk3) in pCMV6 vector (#MR227586) were obtained from OriGene Technologies Inc. … We generated the pCMV6-fNeo1-ECD/TMD, fNeo1-ICD, and fNeo1ΔIg constructs by using pCMV6-fNeo1 as a template, the primers listed in Supplemental Table S1, and the QuikChange site-directed mutagenesis kit (Agilent). …
Hepatocyte Neogenin Is Required for Hemojuvelin-Mediated Hepcidin Expression and Iron Homeostasis in Mice
Enns, Jue, Zhang
Blood (2021)
Cited by 21
AI Summary: OriGene contributed to this study by providing the mouse Neo1 ORF cDNA construct with a C-terminal FLAG/MYC epitope in the pCMV6 vector (pCMV6-fNeo1, #MR226235), which was used to investigate the role of Neo1 in hepcidin expression and iron homeostasis.
Snippet: cDNA constructs. Mouse Neo1 ORF (NM_008684) with a C-terminal FLAG/MYC epitope in pCMV6 vector (pCMV6-fNeo1) was obtained from Origene (#MR226235). pCMV6-fNeo1L1046E mutant was generated as described in Supplemental Information
Showing 1-4 of 4 papers.
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