Keap1 (NM_016679) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225865
Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1
$929.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Keap1 |
| Synonyms | INRF2; mKIAA0132 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016679 |
| ORF Size | 1872 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016679.1, NM_016679.2, NM_016679.3, NM_016679.4, NP_057888.1 |
| RefSeq Size | 4470 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 50868 |
| UniProt ID | Q9Z2X8 |
| Cytogenetics | 9 A3 |
| MW | 70 kDa |
| Summary | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016679" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC219819 | Keap1 (untagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1, (10ug) | 10 ug |
$872.00
|
|
| MG209525 | Keap1 (tGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1) | 10 ug |
$1,072.00
|
|
| MG225865 | Keap1 (tGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1) transcript variant 1, (10ug) | 10 ug |
$1,129.00
|
|
| MR225865L1 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1 | 10 ug |
$1,229.00
|
|
| MR225865L2 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1 | 10 ug |
$1,229.00
|
|
| MR225865L3 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1 | 10 ug |
$1,229.00
|
|
| MR225865L4 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 1 | 10 ug |
$1,229.00
|
|
Citations
Analysis of the role of Krebs cycle rewiring in macrophage cytokine production
Ryan
Thesis (2019)
Ryan
Thesis (2019)
Snippet: … Analysis of the role of Krebs cycle rewiring in macrophage cytokine production Thesis submitted to the University of Dublin for the Degree of Doctor of Philosophy by Dylan Gerard Ryan School of Biochemistry and Immunology Trinity Biomedical Sciences View as HTML;Number: 4390771, Assay ID: s70521 and s70522) and scrambled control (Catalogue Number: 4390843). MYC-DDK-tagged KEAP1 (Catalogue number: MR225865) was from Origene. …
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
