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Per1 (NM_011065) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225742
Per1 (Myc-DDK-tagged) - Mouse period homolog 1 (Drosophila) (Per1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,236.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Per1
Synonyms Hftm; m-rigui; mPer1; Per
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225742 representing NM_011065
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGTCCCCTAGAAGGGGCCGATGGGGGAGGAGACCCCAGGCCCGGAGAACCTTTTTGTCCTGGAG
GAGTCCCATCCCCTGGGGCCCCGCAGCACCGGCCTTGTCCAGGCCCCAGCCTGGCTGATGACACTGATGC
AAACAGCAATGGCTCAAGTGGCAATGAGTCCAACGGACCCGAGTCCAGGGGCGCATCTCAGCGGAGTTCT
CATAGTTCCTCTTCTGGCAATGGCAAGGACTCAGCTCTGCTGGAGACCACTGAGAGCAGCAAGAGTACAA
ACTCACAGAGCCCATCCCCACCCAGCAGCTCCATTGCCTACAGCCTCCTGAGTGCGAGCTCAGAGCAGGA
CAACCCATCTACCAGTGGCTGCAGCAGTGAACAGTCAGCTCGAGCCAGGACCCAGAAAGAACTCATGACT
GCACTTCGGGAGCTCAAACTTCGACTGCCACCAGAGCGTCGGGGCAAGGGCCGCTCTGGGACCTTGGCCA
CACTGCAGTACGCTCTGGCCTGTGTCAAGCAGGTTCAGGCTAACCAGGAATATTACCAGCAGTGGAGTCT
GGAGGAGGGTGAGCCTTGTGCCATGGACATGTCTACTTACACCCTGGAGGAATTGGAGCATATCACATCC
GAATACACACTTCGAAACCAGGACACCTTCTCTGTGGCTGTGTCCTTCCTGACAGGCCGGATTGTCTATA
TTTCGGAGCAGGCAGGTGTCCTGCTGCGTTGCAAACGGGATGTGTTTCGGGGTGCCCGCTTCTCAGAGCT
CCTGGCTCCCCAGGATGTGGGTGTCTTCTATGGCTCTACTACACCATCTCGACTGCCCACCTGGGGCACT
GGCACCTCTGCAGGTTCAGGTCTCAAGGACTTCACCCAGGAAAAGTCTGTCTTCTGCCGAATCAGAGGAG
GTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCATATGTGACCAAGATTCGGGT
CTCAGATGGAGCCCCTGCACAGCCGTGCTGCCTACTCATTGCCGAGCGCATCCACTCTGGTTATGAAGCT
CCCCGGATCCCTCCTGACAAGAGGATCTTCACCACCCGACACACACCAAGCTGCCTCTTCCAGGATGTAG
ATGAAAGGGCTGCCCCACTGCTGGGTTACCTTCCCCAGGATCTCCTGGGGGCTCCAGTACTTCTCTTTCT
ACATCCTGAGGACCGACCCCTCATGCTGGCCATTCATAAGAAGATACTGCAGCTGGCAGGCCAGCCCTTT
GACCATTCCCCTATTCGCTTCTGTGCTCGGAACGGGGAATATGTCACCATGGACACCAGCTGGGCCGGTT
TTGTGCACCCCTGGAGCCGCAAGGTGGCTTTCGTGTTGGGTCGCCATAAAGTGCGCACGGCACCCCTGAA
TGAGGACGTCTTCACTCCCCCAGCCCCCAGCCCAGCTCCGTCCCTGGACTCTGATATCCAGGAGCTCTCA
GAGCAGATCCATCGATTGCTGCTGCAGCCTGTGCACAGCTCCAGCCCCACGGGGCTCTGTGGAGTTGGCC
CTCTGATGTCCCCTGGTCCTCTACACAGCCCTGGCTCCTCCAGTGATAGCAATGGGGGGGACGCTGAGGG
GCCTGGGCCTCCTGCTCCAGTGACTTTCCAGCAGATCTGTAAGGATGTGCATCTGGTAAAGCACCAGGGA
CAACAGCTCTTCATTGAATCTCGGGCCAAGCCCCCACCCCGGCCCCGCCTCCTTGCTACAGGTACATTCA
AAGCCAAAGTCCTTCCCTGCCAGTCCCCAAACCCCGAACTGGAGGTGGCCCCAGTTCCTGACCAAGCCTC
GTTAGCCTTGGCCCCTGAGGAGCCAGAGAGGAAAGAAACCTCTGGCTGTTCCTACCAGCAGATCAACTGC
CTGGACAGCATCCTCAGGTATTTGGAGAGCTGCAACATTCCCAGTACAACCAAGCGTAAATGTGCCTCCT
CCTCCTCCTACACTGCCTCTTCAGCCTCTGATGATGACAAGCAGAGGGCAGGTCCAGTTCCTGTGGGGGC
CAAGAAAGATCCGTCGTCAGCAATGCTGTCTGGGGAGGGGGCAACTCCTCGGAAGGAGCCAGTGGTGGGA
GGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCAGAGAGCGTGGTGTCCGTCACCAGTCAGTGTAGCT
TCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCCCCGGAGTCGGACATCATCATGATGGAAGACCT
GCCTGGCCTGGCCCCTGGCCCAGCCCCCAGTCCGGCCCCCAGCCCCACAGTAGCCCCTGACCCAACCCCA
GATGCTTATCGCCCAGTGGGTCTGACCAAGGCCGTGCTGTCCCTGCACACACAGAAGGAAGAGCAAGCCT
TCCTCAACCGCTTCAGAGATCTTGGCAGGCTTCGTGGACTTGACACCTCTTCTGTGGCCCCCTCAGCCCC
TGGCTGCCACCATGGCCCCATTCCCCCTGGTCGCCGACACCACTGCCGATCTAAAGCAAAGCGTTCCCGC
CACCACCACCACCAGACCCCCCGGCCCGAAACTCCCTGCTATGTCTCCCATCCTTCACCTGTGCCCTCTT
CTGGACCCTGGCCACCCCCACCAGCCACGACCCCCTTCCCAGCAATGGTCCAGCCCTACCCACTCCCAGT
ATTCTCCCCTCGAGGAGGACCCCAGCCCCTTCCCCCTGCCCCTACATCTGTGTCCCCTGCTACCTTCCCT
TCTCCCTTAGTGACCCCAATGGTGGCCTTGGTGCTCCCTAACTATCTATTCCCTACCCCACCTAGTTATC
CATATGGGGTGTCCCAGGCCCCTGTTGAGGGGCCACCCACGCCTGCTTCCCACTCGCCCTCTCCATCCCT
GCCCCCACCACCTCTCAGCCCCCCCCACCGCCCAGACTCCCCACTGTTCAACTCGAGATGCAGCTCCCCA
CTCCAGCTCAATCTGCTGCAGCTTGAGGAGTCCCCCCGCACGGAGGGGGGCGCTGCTGCAGGAGGCCCAG
GAAGCAGTGCTGGGCCCCTGCCTCCCAGTGAGGAGACTGCTGAGCCAGAGGCCAGATTGGTGGAGGTTAC
TGAGTCGTCCAATCAGGATGCACTTTCAGGCTCCAGCGACCTGCTGGAGCTACTGCTCCAAGAAGACTCT
CGCTCGGGCACAGGCTCCGCAGCCTCAGGCTCCCTGGGCTCTGGCCTGGGCTCTGGGTCTGGTTCAGGAT
CCCACGAAGGGGGAAGCACCTCAGCCAGCATCACCCGCAGCAGTCAGAGCAGCCATACAAGCAAGTACTT
TGGCAGCATCGACTCTTCCGAGGCTGAAGCTGGGGCTGCTCGGGCCAGGACTGAGCCTGGGGACCAGGTC
ATTAAGTGTGTGCTCCAGGACCCCATCTGGCTGCTCATGGCCAATGCCGACCAGCGTGTCATGATGACAT
ACCAGGTGCCGTCCAGGGATGCAGCCTCTGTGCTGAAGCAAGACCGGGAGAGGCTCCGGGCCATGCAGAA
ACAGCAGCCACGGTTCTCAGAGGACCAGAGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAGGGC
CAGCTGCCTCGGGCCCTTGATGTGACGGCGTGTGTGGACTGTGGCAGCAGCGTTCAAGATCCTGGCCACT
CTGATGACCCCCTCTTCTCAGAACTGGATGGATTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGAGGG
TGGTGGGTGTGGTGTTGGCGGTGGTGGGGGTGATGGTGGTGAGGAGGCCCAGACCCAAATTGGGGCTAAG
GGTTCAAGCTCTCAGGACTCTGCCATGGAGGAAGAAGAGCAAGGTGGGGGCTCATCCAGCCCAGCTTTAC
CTGCAGAAGAAAACAGCACCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225742 representing NM_011065
Red=Cloning site Green=Tags(s)

MSGPLEGADGGGDPRPGEPFCPGGVPSPGAPQHRPCPGPSLADDTDANSNGSSGNESNGPESRGASQRSS
HSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMT
ALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCAMDMSTYTLEELEHITS
EYTLRNQDTFSVAVSFLTGRIVYISEQAGVLLRCKRDVFRGARFSELLAPQDVGVFYGSTTPSRLPTWGT
GTSAGSGLKDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAERIHSGYEA
PRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPEDRPLMLAIHKKILQLAGQPF
DHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAPSLDSDIQELS
EQIHRLLLQPVHSSSPTGLCGVGPLMSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG
QQLFIESRAKPPPRPRLLATGTFKAKVLPCQSPNPELEVAPVPDQASLALAPEEPERKETSGCSYQQINC
LDSILRYLESCNIPSTTKRKCASSSSYTASSASDDDKQRAGPVPVGAKKDPSSAMLSGEGATPRKEPVVG
GTLSPLALANKAESVVSVTSQCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPTP
DAYRPVGLTKAVLSLHTQKEEQAFLNRFRDLGRLRGLDTSSVAPSAPGCHHGPIPPGRRHHCRSKAKRSR
HHHHQTPRPETPCYVSHPSPVPSSGPWPPPPATTPFPAMVQPYPLPVFSPRGGPQPLPPAPTSVSPATFP
SPLVTPMVALVLPNYLFPTPPSYPYGVSQAPVEGPPTPASHSPSPSLPPPPLSPPHRPDSPLFNSRCSSP
LQLNLLQLEESPRTEGGAAAGGPGSSAGPLPPSEETAEPEARLVEVTESSNQDALSGSSDLLELLLQEDS
RSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARARTEPGDQV
IKCVLQDPIWLLMANADQRVMMTYQVPSRDAASVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKG
QLPRALDVTACVDCGSSVQDPGHSDDPLFSELDGLGLEPMEEGGGEGGGCGVGGGGGDGGEEAQTQIGAK
GSSSQDSAMEEEEQGGGSSSPALPAEENSTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011065
ORF Size 3873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011065.5
RefSeq Size 4663 bp
RefSeq ORF 3876 bp
Locus ID 18626
UniProt ID O35973
Cytogenetics 11 B3
MW 136.8 kDa
Summary This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene may increase the risk of getting certain cancers. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jan 2014
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Documents
Resources
"NM_011065" in other vectors (4)
SKU Description Size Price
MC224163 Per1 (untagged) - Mouse period homolog 1 (Drosophila) (Per1), transcript variant 1, (10ug) 10 ug
$1,237.00
MG225742 Per1 (tGFP-tagged) - Mouse period homolog 1 (Drosophila) (Per1) transcript variant 1, (10ug) 10 ug
$789.00 $1,436.00
MR225742L3 Lenti ORF clone of Per1 (Myc-DDK-tagged) - Mouse period homolog 1 (Drosophila) (Per1), transcript variant 1 10 ug
$1,536.00
MR225742L4 Lenti ORF clone of Per1 (mGFP-tagged) - Mouse period homolog 1 (Drosophila) (Per1), transcript variant 1 10 ug
$1,536.00

Plasmid Map for MR225742

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225742
Circular map for MR225742

file-alt Citations

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