Far2 (NM_178797) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR225671
Far2 (Myc-DDK-tagged) - Mouse fatty acyl CoA reductase 2 (Far2)
  $701.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Far2
Synonyms A230046P18; AW048109; BC055759; Mlstd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225671 representing NM_178797
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATGATCGCAGCTTTCTACAGCAACAAGTCTATCCTCATCACAGGGGCCACAGGCTTCCTGGGCA
AGGTGCTCATGGAGAAGTTATTCCGTACCAGCCCCCACTTGAAAGTCATTTACATCCTCGTGAGACCCAA
GTCTGGACAGACACTGCAGGAAAGGGTTTTCCAGATCCTCAACAGTAAGCTGTTTGAGAAAGTCAAAGAA
GTCTGTCCAAATGTGCATGAGAAGATCAGACCCATCTCCGCAGACCTCAACCAGCGTGACTTCGCCATCA
GCAAGGAAGACGTGCAAGAACTTCTGTCCTGTACCAATATTATCTTCCACTGTGCAGCCACAGTGCGCTT
TGATGCACATCTGAGAGAAGCTGTGCAGCTGAACGTCACGGCCACCCAGCAGCTCCTGCTAATGGCCAGC
CAGATGCCAAAGCTGGAAGCCTTCATCCACATCTCCACTGCCTTTTCCAACTGCAACCTGAGCCACATTG
ATGAGGTCATCTACCCATGCCCTGTGGAGCCAAGAAAGATCATTGACTCCATGGAGTGGTTAGATGACTC
TATCATTGAAGAGATCACTCCTAAGCTGATTGGGGACCGGCCCAATACCTACACCTACACCAAAGCCTTG
GGAGAGATAGTAGTGCAGCAAGAGAGCGGAAACCTGAATGTGGCCATTGTCAGGCCATCCATAGTGGGAG
CAACCTGGCAGGAGCCTTTCCCGGGTTGGGTTGATAATCTAAATGGACCCAGCGGACTCATTATTGCGAC
TGGGAAAGGATTTCTTCGGTCCATAAAAGCGACTCCCATGGCAGTGGCAGATGTGATTCCAGTTGACACA
GTTGTTAATCTCACCATAGCAGTAGGATGGTATACTGCAGTTCATAGGCCTAAATCAACATTAATCTACC
ATTCCACATCTGGTAATCTCAATCCCTGTAACTGGTATAAAATGGGATTACAGGTCTTGGCGACCATCGA
AAAGATCCCATTTGAGAGTGCTTTCAGAAGGCCAAATGCTGACTTCACCACCAGCAATTTCACCACCCAT
TATTGGAACACCGTCAGCCACCGGGTCCCAGCCATCATCTATGACTTCTATCTGAGGCTTACTGGAAGGA
AACCCAGAATGCTGAAGCTCATGAACCGGCTGCTAAAAACCATTTCCATGCTGGAGTATTTCATCAACCA
CAGTTGGGAATGGAGCACAAACAACACGGAGATGCTTCTGTCAGAGCTCAGTCCTGAAGACCAGAGAGTA
TTCAACTTTGATGTGCGTCAGTTGAACTGGCTCGAATACATTGAAAATTACGTTTTGGGGGTTAAGAAGT
ATCTACTAAAAGAGGATCTGGCCGGTATCCCCAAAGCAAAGCAACACCTAAGAAGGCTCCGGAACATTCA
CTACCTCTTTAACACTGCCCTGTTTCTTATCATCTGGCGCCTCCTCATTGCAAGGTCTCAGATGGCTCGG
AACGTCTGGTTCTTCATTTGTCCCTTGTTCAAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR225671 representing NM_178797
Red=Cloning site Green=Tags(s)

MSMIAAFYSNKSILITGATGFLGKVLMEKLFRTSPHLKVIYILVRPKSGQTLQERVFQILNSKLFEKVKE
VCPNVHEKIRPISADLNQRDFAISKEDVQELLSCTNIIFHCAATVRFDAHLREAVQLNVTATQQLLLMAS
QMPKLEAFIHISTAFSNCNLSHIDEVIYPCPVEPRKIIDSMEWLDDSIIEEITPKLIGDRPNTYTYTKAL
GEIVVQQESGNLNVAIVRPSIVGATWQEPFPGWVDNLNGPSGLIIATGKGFLRSIKATPMAVADVIPVDT
VVNLTIAVGWYTAVHRPKSTLIYHSTSGNLNPCNWYKMGLQVLATIEKIPFESAFRRPNADFTTSNFTTH
YWNTVSHRVPAIIYDFYLRLTGRKPRMLKLMNRLLKTISMLEYFINHSWEWSTNNTEMLLSELSPEDQRV
FNFDVRQLNWLEYIENYVLGVKKYLLKEDLAGIPKAKQHLRRLRNIHYLFNTALFLIIWRLLIARSQMAR
NVWFFICPLFKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178797
ORF Size 1506 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178797.4
RefSeq Size 3397 bp
RefSeq ORF 1509 bp
Locus ID 330450
UniProt ID Q7TNT2
Cytogenetics 6 G3
MW 58.1 kDa
Summary Catalyzes the reduction of saturated but not unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols. A lower activity can be observed with shorter fatty acyl-CoA substrates (PubMed:15220348). It may play a role in the production of ether lipids/plasmalogens and wax monoesters which synthesis requires fatty alcohols as substrates (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178797" in other vectors (4)
SKU Description Size Price
MC217081 Far2 (untagged) - Mouse fatty acyl CoA reductase 2 (Far2), (10ug) 10 ug
$749.00
MG225671 Far2 (tGFP-tagged) - Mouse fatty acyl CoA reductase 2 (Far2), (10ug) 10 ug
$901.00
MR225671L3 Lenti ORF clone of Far2 (Myc-DDK-tagged) - Mouse fatty acyl CoA reductase 2 (Far2) 10 ug
$1,001.00
MR225671L4 Lenti ORF clone of Far2 (mGFP-tagged) - Mouse fatty acyl CoA reductase 2 (Far2) 10 ug
$1,001.00

Plasmid Map for MR225671

Download sequence for

Cloning scheme for MR225671

Circular map for MR225671

file-alt Citations

FAR2 is Associated with Kidney Disease in Mice and Humans
Backer, Eddy, Sheehan et al
Physiol. Genomics (2018) 50 (8), 543-552 DOI: 10.1152/physiolgenomics.00118.2017
AI Summary: OriGene products, including purified human NKX3-2 protein (RC211285) and the pCMV6 plasmid containing mouse Far2 cDNA clone with a Myc/DDK-tag (MR225671), were used to investigate the molecular mechanisms by which FAR2 regulates mesangial matrix expansion in kidney disease.
Snippet: The assay was repeated using 74 purified human NKX3-2 protein (Origene RC211285) established by transfecting MES13 cells with the pCMV6 plasmid (OriGene) containing 80 the mouse Far2 cDNA clone NM_178797 with a Myc/DDK-tag (MR225671, OriGene) 81
Showing 1-1 of 1 papers.
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