Opa1 (NM_133752) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225663
Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2
$589.00
$880.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Opa1 |
| Synonyms | 1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_133752 |
| ORF Size | 2880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_133752.1 |
| RefSeq Size | 5948 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 74143 |
| UniProt ID | P58281 |
| Cytogenetics | 16 20.65 cM |
| MW | 111.3 kDa |
| Summary | Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_133752" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC222838 | Opa1 (untagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) | 10 ug |
$968.00
|
|
| MG225663 | Opa1 (tGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1) nuclear gene encoding mitochondrial protein, (10ug) | 10 ug |
$1,080.00
|
|
| MR225663L3 | Lenti ORF clone of Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$1,180.00
|
|
| MR225663L4 | Lenti ORF clone of Opa1 (mGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 | 10 ug |
$1,180.00
|
|
Citations
Caspase-3 cleaved tau impairs mitochondrial function through the opening of the mitochondrial permeability transition pore
Pérez, Ibarra-García-Padilla, Tang et al
Biochim Biophys Acta Mol Basis Dis (2023) 1870 (1), 166898 DOI: 10.1016/j.bbadis.2023.166898
Pérez, Ibarra-García-Padilla, Tang et al
Biochim Biophys Acta Mol Basis Dis (2023) 1870 (1), 166898 DOI: 10.1016/j.bbadis.2023.166898
Cited by 24
Showing 1-1 of 1 papers.
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