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Ankrd44 (NM_001081433) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225641
Ankrd44 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 44 (Ankrd44)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $910.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd44
Synonyms 4930444A19Rik; A130096K20; E130014H08Rik; PP6-ARS-B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225641 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTGCTCAAACTCTGCGAGCAGCCACCACTGGTCCAGGCAATCTTCAGTGGCGACCCAGAGGAGA
TTCGCATGCTCATCCATAAGACTGAAGACGTGAACGCACTGGATTCTGAGAAACGAACCCCGCTTCATGT
GGCTGCATTCCTGGGAGATGCAGAGATCATTGAACTCCTGATTTTGTCAGGAGCTCGTGTAAATGCCAAG
GACAACATGTGGCTGACCCCACTGCACCGGGCTGTTGCTTCCAGAAGTGAAGAGGCAGTGCAAGTATTGA
TTAAACACTCGGCTGATGTCAATGCAAGGGACAAGAATTGGCAGACCCCCCTCCATGTGGCAGCAGCCAA
TAAGGCCGTCAAGTGTGCAGAAGTGATCATTCCACTACTGAGCAGTGTCAACGTCTCCGACAGAGGCGGG
CGCACAGCCTTGCACCACGCCGCTCTGAATGGACACATGGAGATGGTCAATTTACTCTTGGCCAAAGGGG
CAAACATCAATGCATTTGACAAGAAGGACCGACGAGCTCTCCACTGGGCAGCATACATGGGTCACCTGGA
CGTTGTGGCGCTGCTCATTAACCATGGTGCAGAAGTTACCTGCAAGGATAAGAAGGGCTACACCCCGCTG
CACGCTGCAGCCTCCAATGGGCAGATCAGTGTTGTCAAACATCTCCTGAACCTGGGGGTGGAGATTGATG
AAATCAATGTCTATGGAAACACAGCACTTCACATTGCCTGCTACAATGGCCAGGATGCTGTCGTTAATGA
GCTGATCGACTATGGTGCTAACGTGAATCAGCCCAACAACAGTGGCTTTACCCCTCTGCACTTTGCAGCG
GCGTCTACTCACGGTGCTCTGTGTCTGGAGTTGTTAGTGAACAATGGGGCCGATGTGAACATTCAGAGTA
AGGATGGCAAAAGTCCTCTGCACATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCA
GAATGGAGGTGAGATCGACTGTGTGGATAAGGATGGCAACACACCTCTCCATGTGGCTGCGAGACATGGT
CACGAGCTCCTGATTAACACCCTAATCACTAGCGGAGCCGACACAGCCAAGTGTGGAATCCATAGCATGT
TCCCCCTACATTTAGCCGCCCTAAATGCTCACTCTGACTGCTGCAGGAAGTTGTTATCATCTGGCTTTGA
AATAGACACCCCAGATACGTTTGGAAGAACGTGCCTCCACGCCGCTGCTGCAGGAGGCAATGTAGAATGC
ATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACT
ATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAAC
AGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTA
GGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTAT
GTCTAGAATTTCTGCTTCAAAATGATGCAAACCCATCCATCCGGGACAAGGAAGGCTACAATAGCATCCA
CTATGCTGCTGCCTATGGCCACAGACAGTGTCTAGAACTGCTTTTGGAAAGAACCAACACTGGCTTTGAA
GAATCAGATGGCGGTGCTCTCAAGAGTCCACTCCACTTAGCAGCATACAATGGGCATCACCAAGCCTTGG
AGGTCCTTCTCCAGTCCCTAGTGGACCTGGACATCAGGGATGAGAAAGGCCGGACAGCTCTGTACCTAGC
TGCTTTCAAGGGACACACAGAATGTGTGGAGGCACTTGTTAACCAGGGCGCATCCATCTTTGTAAAGGAC
AATGTCACCAAAAGAACTCCACTTCATGCTTCAGTTATTAATGGTCACACACTGTGTTTGAGGCTACTAC
TAGAAACAGCAGACAATCCGGAAGTGGTTGATGTGAAAGATGCCAAGGGACAAACCCCGCTGATGCTTGC
AGTAGCATATGGACACATTGATGCTGTTTCATTGTTACTTGAAAAGGAAGCAAACGTAGATGCGGTCGAC
ATAGTGGGATGCACGGCTTTACATAGAGGGATTATGACAGGACATGAGGAATGTGTGCAAATGCTGCTGG
AACAAGAAGCATCAATCCTCTGTAAAGATTCCAGAGGGAGGACACCCTTGCACTATGCGGCTGCTCGGGG
CCACGCCACATGGCTGAATGAATTGCTCCAGATTGCCCTTTCTGAAGAAGACTGCTGTCTCAAAGACAAC
CAGGGATACACGCCACTGCACTGGGCGTGTTACAATGGTAATGAAAACTGCATAGAGGTACTTTTGGAGC
AAAAATGTTTTCGAAAATTTATTGGTAATCCCTTCACTCCACTGCACTGTGCAATAATAAATGGTCACGA
GAGCTGTGCATCATTGCTCCTGGGGGCCATAGATCCCAGCATTGTCAGCTGCAGGGATGACAAAGGCAGG
ACAACCCTCCACGCGGCAGCCTTTGGAGATCATGCGGAGTGCTTGCAGCTGCTTCTGAGACATGATGCTC
AAGTGAATGCCGTAGATAATTCAGGGAAAACAGCACTGATGATGGCCGCTGAGAACGGGCAGGCAGGAGC
TGTGGATATTTTGGTGAACAGTGCCCAGGCTGACCTGACTGTGAAGGATAAGGACTTGAATACTCCCTTA
CACCTGGCTATCAGTAAAGGCCATGAGAAATGTGCCTTGTTAATACTTGACAAGATACAAGACGAGAGCC
TTATCAATGCGAAGAACAGTGCGCTGCAGACACCCCTCCATATCGCTGCACGCAATGGCTTGAAGGTGGT
AGTTGAGGAGTTGCTGGCGAAAGGAGCCTGCGTACTTGCTGTAGATGAAAATGCTTCTAGGTCGAATGGA
CCCCGTTCTCCACCTGGAACGGCTGTACGAAAGGAAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225641 protein sequence
Red=Cloning site Green=Tags(s)

MAVLKLCEQPPLVQAIFSGDPEEIRMLIHKTEDVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAK
DNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGG
RTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPL
HAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANVNQPNNSGFTPLHFAA
ASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARHG
HELLINTLITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDTFGRTCLHAAAAGGNVEC
IKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANVNETDDWGRTALHYAAASDMDRNKMIL
GNAHDNSEELERAREVKEKDAALCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELLLERTNTGFE
ESDGGALKSPLHLAAYNGHHQALEVLLQSLVDLDIRDEKGRTALYLAAFKGHTECVEALVNQGASIFVKD
NVTKRTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVD
IVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGHATWLNELLQIALSEEDCCLKDN
QGYTPLHWACYNGNENCIEVLLEQKCFRKFIGNPFTPLHCAIINGHESCASLLLGAIDPSIVSCRDDKGR
TTLHAAAFGDHAECLQLLLRHDAQVNAVDNSGKTALMMAAENGQAGAVDILVNSAQADLTVKDKDLNTPL
HLAISKGHEKCALLILDKIQDESLINAKNSALQTPLHIAARNGLKVVVEELLAKGACVLAVDENASRSNG
PRSPPGTAVRKEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081433
ORF Size 2979 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081433.3, NP_001074902.2
RefSeq Size 6156 bp
RefSeq ORF 2982 bp
Locus ID 329154
UniProt ID B2RXR6
Cytogenetics 1 C1.2
MW 107.4 kDa
Summary Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081433" in other vectors (4)
SKU Description Size Price
MC223020 Ankrd44 (untagged) - Mouse ankyrin repeat domain 44 (Ankrd44), (10ug) 10 ug
$1,001.00
MG225641 Ankrd44 (tGFP-tagged) - Mouse ankyrin repeat domain 44 (Ankrd44), (10ug) 10 ug
$1,110.00
MR225641L3 Lenti ORF clone of Ankrd44 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 44 (Ankrd44) 10 ug
$1,210.00
MR225641L4 Lenti ORF clone of Ankrd44 (mGFP-tagged) - Mouse ankyrin repeat domain 44 (Ankrd44) 10 ug
$1,210.00

Plasmid Map for MR225641

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225641
Circular map for MR225641

file-alt Citations

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