Kcmf1 (NM_019715) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR225601
Kcmf1 (Myc-DDK-tagged) - Mouse potassium channel modulatory factor 1 (Kcmf1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcmf1
Synonyms 1700094M07Rik; Debt91; Pmcf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225601 representing NM_019715
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGACATGAAGGTGTCAGCTGTGATGCATGTTTAAAAGGAAATTTTCGAGGTCGCAGATATAAGT
GTTTAATTTGCTACGATTACGATCTTTGTGCATCTTGTTATGAAAGCGGGGCAACGACAACAAGGCATAC
AACTGACCACCCGATGCAGTGCATATTAACAAGGGTAGACTTTGATTTGTACTATGGTGGGGAAGCTTTC
TCTGTAGAGCAGCCACAGTCTTTTACTTGTCCCTATTGTGGAAAAATGGGCTACACGGAAACATCTCTTC
AAGAACATGTTACTTCTGAACACGCAGAAACATCAACAGAAGTGATTTGTCCCATATGTGCAGCGTTACC
TGGAGGAGATCCTAATCATGTCACAGATGACTTTGCAGCTCATCTGACACTTGAACACAGAGCCCCTAGA
GATTTAGATGAATCGAGTGGTGTTCGACATGTACGTAGAATGTTTCACCCTGGGCGGGGGCTAGGAGGCC
CTCGTGCTCGGAGATCAAACATGCACTTTACTAGCAGTTCTACTGGTGGACTCTCTTCTTCTCAGAGTTC
ATACTCTCCAAGCAGTAGGGAAGCCATGGATCCTATAGCTGAGCTGTTATCTCAGTTATCAGGAGTGAGA
CGTTCTGCAGGGGGACAGCTTAACTCTTCAGGCCCTTCCGCTTCTCAGTTGCAACAACTGCAGATGCAGC
TGCAGCTTGAGCGACAGCATGCACAGGCAGCCCGGCAACAACTGGAGACTGCTCGCAACGCAAGTCGGCG
CACTAACACAAGCAGCGTCACCACTACGATCACACAGGCCACGGCCACAGCCAACACAGCCAACACAGAG
AACAGCCCGCAGGCCCTCCACAACTCCCAGTTCCTGTTAACAAGGTTGAATGATCCCAAAATGTCTGAAG
CAGAGCGCCAGTCCATGGAGAGCGAGCGTGCGGACCGCAGCCTGTTTGTCCAGGAGCTCCTTCTTTCCAC
GTTAGTGCGCGAAGAGAGCTCGTCCTCAGATGAGGATGATCGGGGGGAGATGGCAGATTTTGGTGCTATG
GGCTGTGTAGATATTATGCCTTTAGATGTTGCTTTAGAAAACCTAAATTTAAAAGAGAGTAATAAAGGAA
ATGAGCCTCCACCACCTCCTCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR225601 representing NM_019715
Red=Cloning site Green=Tags(s)

MSRHEGVSCDACLKGNFRGRRYKCLICYDYDLCASCYESGATTTRHTTDHPMQCILTRVDFDLYYGGEAF
SVEQPQSFTCPYCGKMGYTETSLQEHVTSEHAETSTEVICPICAALPGGDPNHVTDDFAAHLTLEHRAPR
DLDESSGVRHVRRMFHPGRGLGGPRARRSNMHFTSSSTGGLSSSQSSYSPSSREAMDPIAELLSQLSGVR
RSAGGQLNSSGPSASQLQQLQMQLQLERQHAQAARQQLETARNASRRTNTSSVTTTITQATATANTANTE
NSPQALHNSQFLLTRLNDPKMSEAERQSMESERADRSLFVQELLLSTLVREESSSSDEDDRGEMADFGAM
GCVDIMPLDVALENLNLKESNKGNEPPPPPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019715
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019715.2, NP_062689.2
RefSeq Size 3251 bp
RefSeq ORF 1146 bp
Locus ID 74287
UniProt ID Q80UY2
Cytogenetics 6 32.3 cM
MW 42.2 kDa
Summary Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019715" in other vectors (4)
SKU Description Size Price
MC211513 Kcmf1 (untagged) - Mouse potassium channel modulatory factor 1 (Kcmf1), (10ug) 10 ug
$503.00
MG225601 Kcmf1 (tGFP-tagged) - Mouse potassium channel modulatory factor 1 (Kcmf1), (10ug) 10 ug
$657.00
MR225601L3 Lenti ORF clone of Kcmf1 (Myc-DDK-tagged) - Mouse potassium channel modulatory factor 1 (Kcmf1) 10 ug
$757.00
MR225601L4 Lenti ORF clone of Kcmf1 (mGFP-tagged) - Mouse potassium channel modulatory factor 1 (Kcmf1) 10 ug
$757.00

Plasmid Map for MR225601

Download sequence for

Cloning scheme for MR225601

Circular map for MR225601

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