OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

P4htm (NM_028944) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR225578
P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $469.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "P4htm or NM_028944"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol P4htm
Synonyms 4933406E20Rik; AI853847; BB128974; Ph-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGCGGTGGCGACCGTGCAGCGGCCTGAGGCTGAGACAGTCGAGGAGGCCTCAAATCTTCAGT
GGCCGCTACCACCGGAGCATCGCCCTTCCGGGGCAGCGACCAGACCAGGCGATAGTGAAGACGCCCCGGT
GCGGCCACTGTGCAAGCCGCGGGGCATCTGTTCCCGTGCCTACTTCTTGGTGCTGATGGTGTTCGTGCAC
CTGTACTTGGGCAACGTGCTGGCCCTGCTGCTCTTCGTGCACTACAGCAACGGAGACGAGAGCACAGACC
CCGGACCCCAGCGCCGCGAGCAGAGCCCCCAGCCAGTGCCAACCTTGGGTCCCCTCACCCGGCTGGAGGG
CATCAAGGTGGGATATGAGCGGAAAGTCCAGGTGGTCGCGGGCAGGGATCACTTCATCCGAACCCTCAGC
CTCAAGCCGCTGCTTTTTGAAATCCCAGGCTTCCTGAGTGACGAGGAGTGTCGGCTCATCATCCACCTGG
CACAGATGAAAGGGTTACAGCGAAGCCAGATCCTGCCCACTGAGGAGTATGAGGAGGCGATGAGCGCCAT
GCAGGTCAGCCAGCTGGACCTCTTCCAGCTGTTGGATCAGAACCACGACGGTCGCCTGCAGCTCCGAGAG
GTCCTGGCCCAGACTCGCCTGGGAAATGGACGGTGGATGACTCCAGAGAACATTCAGGAGATGTACTCTG
CAATCAAAGCAGACCCTGACGGTGATGGCGTGCTGAGTCTGCAGGAATTTTCCAACATGGACCTCCGAGA
CTTCCACAAGTACATGAGGAGCCATAAGGCAGAATCCAATGAGCTGGTGAGGAACAGCCACCACACATGG
CTCCACCAGGGAGAGGGAGCACATCACGTCATGCGTGCCATCCGCCAGAGGGTCCTGCGCCTCACTCGCC
TCTCCCCGGAGATTGTGGAGTTCAGTGAGCCACTACAGGTTGTACGATATGGTGAGGGAGGTCACTACCA
TGCCCATGTGGACAGCGGACCCGTGTACCCAGAGACCATCTGCTCCCATACCAAGCTGGTAGCCAATGAA
TCTGTACCGTTTGAGACCTCCTGTCGCTACATGACAGTGCTGTTTTATTTGAACAATGTCACCGGTGGGG
GTGAGACCGTCTTCCCTGTGGCAGACAACAGGACCTATGATGAAATGAGTCTGATTCAGGATGATGTCGA
TCTCCGGGACACTCGAAGGCACTGTGACAAGGGCAACCTTCGTGTCAAGCCCCAGCAAGGAACTGCAGTC
TTTTGGTACAACTACCTTCCTGATGGGCAAGGCTGGGTGGGTGAAGTGGACGACTACTCGTTGCATGGGG
GCTGCCTGGTCACCCGCGGCACCAAGTGGATCGCTAACAACTGGATCAATGTGGACCCTAGCCGGGCACG
GCAAGCCTTGTTCCAGCAGGAGATGGCTCGTCTGGCTCGGGAAGGTGGCATGGACTCACAACCCGAGTGG
GCCTTGGACCGAGCCTACAGTGATGCCCGTGTGGAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225578 protein sequence
Red=Cloning site Green=Tags(s)

MAAAVATVQRPEAETVEEASNLQWPLPPEHRPSGAATRPGDSEDAPVRPLCKPRGICSRAYFLVLMVFVH
LYLGNVLALLLFVHYSNGDESTDPGPQRREQSPQPVPTLGPLTRLEGIKVGYERKVQVVAGRDHFIRTLS
LKPLLFEIPGFLSDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSAMQVSQLDLFQLLDQNHDGRLQLRE
VLAQTRLGNGRWMTPENIQEMYSAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESNELVRNSHHTW
LHQGEGAHHVMRAIRQRVLRLTRLSPEIVEFSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANE
SVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAV
FWYNYLPDGQGWVGEVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGMDSQPEW
ALDRAYSDARVEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028944
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028944.4
RefSeq Size 1810 bp
RefSeq ORF 1512 bp
Locus ID 74443
UniProt ID Q8BG58
Cytogenetics 9 F2
MW 57 kDa
Summary Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_028944" in other vectors (4)
SKU Description Size Price
MC217025 P4htm (untagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), (10ug) 10 ug
$516.00
MG225578 P4htm (tGFP-tagged) - Mouse prolyl 4-hydroxylase transmembrane (endoplasmic reticulum) (P4htm), (10ug) 10 ug
$669.00
MR225578L3 Lenti ORF clone of P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm) 10 ug
$769.00
MR225578L4 Lenti ORF clone of P4htm (mGFP-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm) 10 ug
$769.00

Plasmid Map for MR225578

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225578
Circular map for MR225578

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products