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Gpr50 (NM_010340) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225562
Gpr50 (Myc-DDK-tagged) - Mouse G-protein-coupled receptor 50 (Gpr50)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $551.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr50
Synonyms H9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225562 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGGTCCCCAAGAGCAACATGGGACCTACAAAGGCGGTTCCCACCCCATTCGGCTGCATTGGCT
GTAAGCTGCCAAAGCCCGACTACCCGCCAGCTCTAATCATCTTCATGTTCTGCGCAATGGTCATCACAGT
CGTCGTAGACCTGATCGGGAACTCCATGGTCATTTTGGCTGTGACCAAGAACAAGAAGCTCCGAAATTCT
GGCAACATCTTTGTGGCCAGCCTCTCTGTGGCAGACATGCTGGTGGCCATCTACCCCTACCCTTTGATGC
TGTATGCCATGTCAGTTGGGGGCTGGGATCTGAGTCAGCTCCAGTGCCAGATGGTCGGGTTGGTCACAGG
ACTGAGCGTAGTCGGTTCCATCTTCAACATTACTGCCATTGCCATCAACCGTTACTGCTACATCTGCCAC
AGCCTCCAATACAAGCGGATCTTCAGCCTGCGCAACACTTGCATCTATCTGGTCGTTACCTGGGTCATGA
CTGTCCTGGCTGTCCTGCCTAACATGTACATTGGCACCATTGAGTATGACCCTCGCACCTACACCTGCAT
CTTCAACTATGTGAACAATCCTGCCTTTACCGTGACCATTGTCTGCATCCACTTCGTCCTCCCTCTCATC
ATAGTTGGTTATTGCTACACGAAAATCTGGATCAAAGTGCTGGCAGCCCGTGACCCAGCTGGACAGAATC
CTGACAACCAGTTTGCTGAGGTTCGAAATTTTCTAACCATGTTTGTGATCTTCCTCCTTTTTGCAGTGTG
CTGGTGCCCTGTCAATGTGCTCACTGTGTTGGTGGCTGTCATTCCAAAGGAAATGGCAGGCAAGATCCCC
AACTGGCTTTATCTTGCAGCCTACTGCATAGCCTACTTCAACAGCTGCCTCAACGCCATCATCTACGGTA
TCCTCAATGAGAGTTTCCGAAGAGAATACTGGACCATCTTCCATGCTATGCGGCACCCTATCCTGTTCAT
CTCTCACCTCATCAGTGATATTCGGGAGACTTGGGAGACCCGAGCTCTCACTCGTGCCCGTGTCCGTGCC
CGTGATCAAGTCCGAGAGCAAGAGCGTGCTCGTGCCTGTGTCGCTGTGGAGGGGACCCCAAGGAACGTCC
GGAATGTTCTACTGCCTGGTGATGCATCAGCACCCCACTCTGATCGTGCCTCTGTCCGTCCCAAGCCCCA
AACCAGGTCTACTTCTGTCTACCGCAAACCTGCCTCTATCCACCACAAGTCTATTTCTGGCCACCCCAAG
TCTGCCTCTGTTTACCCTAAGCCAGCCTCCTCTGTCCATTGCAAGCCTGCCTCTGTCCATTTCAAACCCG
CCTCTGTTCATTTCAAGGGTGACTCTGTCTATTTCAAGGGAGACACTGTCCATTACAGGGCTGCTTCCAA
ACTTGTCACCAGTCACCGTATCTCTGCTGGCCCTTCCACCAGTCACCCTACATCCATGGCTGGCTACATT
AAATCTGGTACCAGCCACCCTGCCACCACCACTGTTGACTATCTCGAACCTGCCACCACCAGCCACTCTG
TGCTCACTGCTGTCGACCTCCCTGAGGTCTCAGCCTCCCATTGCCTTGAGATGACCAGCACTGGCCACCT
CAGAGCTGACATTTCTGCCTCTGTCCTTCCTTCTGTACCCTTCGAGCTTGCTGCCACCCCTCCTGATACC
ACTGCAATCCCCATTGCCTCTGGTGATTACCGCAAGGTCGTGCTTATTGATGATGATTCTGATGATTCTG
ATTGCTCTGATGAGATGGCTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225562 protein sequence
Red=Cloning site Green=Tags(s)

MATVPKSNMGPTKAVPTPFGCIGCKLPKPDYPPALIIFMFCAMVITVVVDLIGNSMVILAVTKNKKLRNS
GNIFVASLSVADMLVAIYPYPLMLYAMSVGGWDLSQLQCQMVGLVTGLSVVGSIFNITAIAINRYCYICH
SLQYKRIFSLRNTCIYLVVTWVMTVLAVLPNMYIGTIEYDPRTYTCIFNYVNNPAFTVTIVCIHFVLPLI
IVGYCYTKIWIKVLAARDPAGQNPDNQFAEVRNFLTMFVIFLLFAVCWCPVNVLTVLVAVIPKEMAGKIP
NWLYLAAYCIAYFNSCLNAIIYGILNESFRREYWTIFHAMRHPILFISHLISDIRETWETRALTRARVRA
RDQVREQERARACVAVEGTPRNVRNVLLPGDASAPHSDRASVRPKPQTRSTSVYRKPASIHHKSISGHPK
SASVYPKPASSVHCKPASVHFKPASVHFKGDSVYFKGDTVHYRAASKLVTSHRISAGPSTSHPTSMAGYI
KSGTSHPATTTVDYLEPATTSHSVLTAVDLPEVSASHCLEMTSTGHLRADISASVLPSVPFELAATPPDT
TAIPIASGDYRKVVLIDDDSDDSDCSDEMAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010340
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010340.2
RefSeq Size 1801 bp
RefSeq ORF 1776 bp
Locus ID 14765
UniProt ID O88495
Cytogenetics X 36.98 cM
MW 65.1 kDa
Summary This gene encodes a multipass membrane protein that is thought to act as a G protein-coupled receptor. Activity of this protein may be important in neurotransmitter and glucocorticoid signalling. Mutation of this gene causes a decreased ability to maintain a constant body temperature, resulting in torpor, as well as an increased metabolic rate. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2015
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Documents
Resources
"NM_010340" in other vectors (4)
SKU Description Size Price
MC219387 Gpr50 (untagged) - Mouse G-protein-coupled receptor 50 (Gpr50), (10ug) 10 ug
$551.00
MG225562 Gpr50 (tGFP-tagged) - Mouse G-protein-coupled receptor 50 (Gpr50), (10ug) 10 ug
$751.00
MR225562L3 Lenti ORF clone of Gpr50 (Myc-DDK-tagged) - Mouse G-protein-coupled receptor 50 (Gpr50) 10 ug
$851.00
MR225562L4 Lenti ORF clone of Gpr50 (mGFP-tagged) - Mouse G-protein-coupled receptor 50 (Gpr50) 10 ug
$851.00

Plasmid Map for MR225562

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225562
Circular map for MR225562

file-alt Citations

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