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Pde10a (NM_011866) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225520
Pde10a (Myc-DDK-tagged) - Mouse phosphodiesterase 10A (Pde10a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $729.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde10a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225520 representing NM_011866
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGATGGACCCTCTAACAATGCGAGTTGCTTCCGAAGGCTGACCGAGTGTTTCCTCAGCCCCAGTT
TGACGGATGAAAAGGTGAAGGCCTATCTTTCTCTCCATCCCCAGGTATTAGATGAATTTGTTTCTGAAAG
TGTTAGTGCAGAGACTGTGGAAAAGTGGCTGAAGAGGAAAACCAACAAAGCAAAAGATGAACCATCTCCC
AAGGAAGTCAGCAGGTACCAGGATACGAATATGCAGGGAGTCGTGTACGAGCTGAACAGCTACATAGAGC
AGCGCCTGGACACGGGCGGGGACAACCACCTGCTCCTCTATGAGCTCAGCAGCATCATCAGGATAGCCAC
AAAAGCCGACGGATTTGCACTGTACTTCCTTGGAGAGTGCAATAATAGCCTGTGTGTGTTCATACCACCC
GGGATGAAGGAAGGCCAACCCCGGCTCATCCCTGCAGGGCCCATCACCCAGGGTACCACCATCTCTGCCT
ACGTGGCCAAGTCTAGGAAGACGTTGTTGGTAGAGGATATCCTTGGGGATGAGCGATTTCCTCGAGGTAC
TGGCCTGGAATCAGGAACCCGCATCCAGTCTGTTCTTTGCTTGCCCATTGTCACTGCCATTGGAGACTTG
ATTGGCATCCTTGAACTGTACAGGCACTGGGGCAAAGAGGCCTTCTGCCTCAGCCATCAGGAGGTTGCAA
CAGCCAATCTTGCTTGGGCTTCCGTAGCAATACACCAGGTGCAGGTGTGTAGAGGTCTCGCCAAACAGAC
CGAACTGAATGACTTCCTACTCGACGTATCAAAGACATACTTTGATAACATAGTTGCCATAGACTCTCTA
CTTGAACACATCATGATATATGCAAAAAATCTAGTGAACGCCGACCGCTGCGCGCTCTTCCAGGTGGACC
ACAAGAACAAGGAGCTGTACTCGGACCTGTTTGACATTGGGGAGGAGAAGGAGGGGAAGCCCATCTTCAA
GAAGACCAAGGAGATCAGATTTTCCATTGAGAAAGGGATTGCTGGTCAAGTGGCAAGAACAGGCGAAGTC
TTGAACATTCCCGATGCCTACGCGGACCCTCGCTTTAACAGGGAGGTGGACCTGTACACAGGCTACACCA
CGAGGAACATTCTGTGTATGCCCATAGTGAGCCGAGGCAGCGTGATTGGCGTGGTGCAGATGGTGAACAA
GATCAGCGGTAGCGCCTTCTCCAAGACAGACGAGAACAACTTCAAGATGTTTGCTGTCTTCTGCGCACTG
GCCTTGCACTGTGCTAACATGTACCACAGGATCCGCCACTCAGAATGCATCTACAGGGTTACCATGGAGA
AGCTTTCCTACCACAGCATCTGCACCTCCGAGGAGTGGCAAGGCCTCATGCGCTTCAACCTACCAGCACG
CATCTGCCGGGACATCGAGCTATTCCACTTTGACATTGGTCCTTTCGAGAACATGTGGCCTGGGATCTTT
GTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAAAAATTGTGCCGTTTTATCATGT
CTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATGCAGTCACGGTGGCACACTGCAT
GTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCGCAAAGGCCTGCTAATTGCGTGT
CTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAGAAGTTCGACCACCCCCTGGCGG
CGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGGTGTCCATCCTTCAGCTGGAAGG
GCACAATATCTTCTCCACCCTGAGCTCCAGCGAGTACGAGCAGGTGCTGGAGATCATCCGCAAAGCCATC
ATCGCCACCGACCTCGCCCTATACTTTGGGAACAGGAAGCAGTTGGAGGAGATGTACCAGACAGGGTCGC
TGAACCTCCACAACCAGTCCCATCGAGACCGTGTCATCGGCTTGATGATGACTGCCTGTGATCTTTGCTC
TGTGACCAAACTATGGCCAGTTACAAAATTGACAGCGAATGATATATATGCAGAATTCTGGGCTGAGGGT
GATGAGATGAAGAAGCTGGGCATACAGCCCATTCCTATGATGGACAGAGACAAGCGAGATGAAGTCCCTC
AAGGGCAGCTCGGATTCTACAATGCTGTGGCCATTCCCTGCTATACCACCTTGACGCAGATCCTCCCACC
CACAGAGCCTCTGCTGAAGGCCTGCAGGGATAACCTCAATCAGTGGGAGAAGGTAATTCGCGGGGAAGAG
ACAGCAATGTGGATTTCAGGCCCAGGCCCGGCGCCTAGCAAGAGCACACCTGAGAAGCTGAACGTGAAGG
TTGAAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011866
ORF Size 2388 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011866.2, NP_035996.2
RefSeq Size 7717 bp
RefSeq ORF 2391 bp
Locus ID 23984
UniProt ID Q8CA95
Cytogenetics 17 A1
MW 90.8 kDa
Summary Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011866" in other vectors (4)
SKU Description Size Price
MC221706 Pde10a (untagged) - Mouse phosphodiesterase 10A (Pde10a), (10ug) 10 ug
$803.00
MG225520 Pde10a (tGFP-tagged) - Mouse phosphodiesterase 10A (Pde10a), (10ug) 10 ug
$929.00
MR225520L3 Lenti ORF clone of Pde10a (Myc-DDK-tagged) - Mouse phosphodiesterase 10A (Pde10a) 10 ug
$1,029.00
MR225520L4 Lenti ORF clone of Pde10a (mGFP-tagged) - Mouse phosphodiesterase 10A (Pde10a) 10 ug
$1,029.00

Plasmid Map for MR225520

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225520
Circular map for MR225520

file-alt Citations

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