Eif2ak2 (NM_011163) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225480
Eif2ak2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2)
$719.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Eif2ak2 |
| Synonyms | 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; Pkr; Prkr; Tik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011163 |
| ORF Size | 1545 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011163.1, NM_011163.2, NM_011163.3, NM_011163.4, NP_035293.1 |
| RefSeq Size | 4343 bp |
| RefSeq ORF | 1548 bp |
| Locus ID | 19106 |
| UniProt ID | Q03963 |
| Cytogenetics | 17 49.56 cM |
| MW | 58.7 kDa |
| Summary | IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including west nile virus (WNV), sindbis virus (SV), foot-and-mouth virus (FMDV), semliki Forest virus (SFV) and lymphocytic choriomeningitis virus (LCMV). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3 and IRS1. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. Regulates proliferation, differentiation and survival of hematopoietic stem/progenitor cells, induction of cytokines and chemokines and plays a role in cortex-dependent memory consolidation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011163" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC217285 | Eif2ak2 (untagged) - Mouse eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), (10ug) | 10 ug |
$528.00
|
|
| MG225480 | Eif2ak2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2), (10ug) | 10 ug |
$919.00
|
|
| MR225480L3 | Lenti ORF clone of Eif2ak2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2) | 10 ug |
$1,019.00
|
|
| MR225480L4 | Lenti ORF clone of Eif2ak2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2-alpha kinase 2 (Eif2ak2) | 10 ug |
$1,019.00
|
|
Citations
The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate
Bhattacharjee, Wang, Anderson et al
EMBO J (2024)
Bhattacharjee, Wang, Anderson et al
EMBO J (2024)
Cited by 10
AI Summary: OriGene contributed to this study by providing murine gene clones (MR207793, MR225480, MR204498, MR227514, MR217037, MR204296, and MR208390), which were used to investigate the role of specific genes in the immune-mediated suppression of nucleic acid synthesis.
Snippet: Clones of human and murine genes were purchased as carboxyl-terminal, FLAG-tagged constructs (OHu27509, OHu00691, OHu13849, OHu19612, OHu18642D, OHu02133D, OHu29487D, OHu03814, and OHu62291 from GenScript and MR207793, MR225480, MR204498, MR227514, MR217037, MR204296, and MR208390 from OriGene)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
