Ccl21c (NM_023052) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR225415
Ccl21c (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 21C (leucine) (Ccl21c)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccl21c
Synonyms 6Ckine; Ccl21b; CKb9; exodus-2; Scya21; Scya21b; Scya21c; SLC; TCA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225415 representing NM_023052
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGATGATGACTCTGAGCCTCCTTAGCCTGGTCCTGGCTCTCTGCATCCCCTGGACCCAAGGCA
GTGATGGAGGGGGACAGGACTGCTGCCTTAAGTACAGCCAGAAGAAAATTCCCTACAGTATTGTCCGAGG
CTATAGGAAGCAAGAACCAAGTTTAGGCTGTCCCATCCCGGCAATCCTGTTCTTACCCCGGAAGCACTCT
AAGCCTGAGCTATGTGCAAACCCTGAGGAAGGCTGGGTGCAGAACCTGATGCGCCGCCTGGACCAGCCTC
CAGCCCCAGGGAAACAAAGCCCCGGCTGCAGGAAGAACCGGGGAACCTCTAAGTCTGGAAAGAAAGGAAA
GGGCTCCAAGGGCTGCAAGAGAACTGAACAGACACAGCCCTCAAGAGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR225415 representing NM_023052
Red=Cloning site Green=Tags(s)

MAQMMTLSLLSLVLALCIPWTQGSDGGGQDCCLKYSQKKIPYSIVRGYRKQEPSLGCPIPAILFLPRKHS
KPELCANPEEGWVQNLMRRLDQPPAPGKQSPGCRKNRGTSKSGKKGKGSKGCKRTEQTQPSRG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023052
ORF Size 399 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023052.2, NP_075539.1
RefSeq Size 887 bp
RefSeq ORF 402 bp
Locus ID 65956
UniProt ID P84443
Cytogenetics 4 22.54 cM
MW 15 kDa
Summary Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023052" in other vectors (4)
SKU Description Size Price
MC210242 Ccl21c (untagged) - Mouse chemokine (C-C motif) ligand 21C (leucine) (Ccl21c), (10ug) 10 ug
$165.00
MG225415 Ccl21c (tGFP-tagged) - Mouse chemokine (C-C motif) ligand 21C (leucine) (Ccl21c), (10ug) 10 ug
$350.00
MR225415L3 Lenti ORF clone of Ccl21c (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 21C (leucine) (Ccl21c) 10 ug
$450.00
MR225415L4 Lenti ORF clone of Ccl21c (mGFP-tagged) - Mouse chemokine (C-C motif) ligand 21C (leucine) (Ccl21c) 10 ug
$450.00

Plasmid Map for MR225415

Download sequence for

Cloning scheme for MR225415

Circular map for MR225415

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