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Ntn4 (NM_021320) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225293
Ntn4 (Myc-DDK-tagged) - Mouse netrin 4 (Ntn4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ntn4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225293 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGCTGCGCACGGCTGCTGCTGCTCTGGGGCTGCTCCGCGGTGGCCGCAGGCTTGAATGGAGTAG
CCGGAGCGAACTCCCGCTGTGAGAAGGCATGCAACCCTCGCATGGGAAACTTGGCTTTGGGAAGAAAGCT
CCGGGCAGACACTATGTGTGGCCAGAACGCCACCGAACTCTTCTGCTTCTACAGTGAGAATGCTGACCTC
ACTTGCCGGCAGCCCAAGTGTGATAAATGCAACGCTGCCCATTCTCACCTAGCTCACCCACCCTCTGCCA
TGGCAGACTCATCCTTCAGGTTTCCCCGGACATGGTGGCAGTCTGCAGAGGATGTGCACAGGGAAAAGAT
TCAGCTAGACCTGGAAGCAGAATTCTACTTCACTCACCTAATTATGGTGTTCAAGTCTCCCAGGCCTGCA
GCCATGGTGCTGGACCGGTCCCAGGACTTTGGGAAGACCTGGAAGCCTTACAAGTACTTTGCAACAAACT
GCTCGGCTACTTTTGGCCTGGAAGATGATGTTGTCAAGAAGGGAGCTATTTGCACGTCTAGATACTCAAA
TCCTTTCCCGTGCACCGGAGGAGAGGTTATTTTCAGAGCCCTGTCACCACCATACGACATAGAAAACCCT
TACAGTGCCAAAGTGCAGGAGCAGCTGAAGATCACCAACCTCCGAGTGCGGCTGCTCAAGCGACAGTCCT
GCCCTTGTCAGATAAACGACCTGAACGCAAAACCTCACCATTTTATGCACTACGCAGTCTATGACTTCAT
CGTCAAGGGCAGCTGCTTCTGCAACGGCCACGCTGACCAGTGCTTACCTGTGGAGGGCTTCAGACCCATC
AAGGCCCCGGGAGCGTTCCACGTGGTCCACGGGAGGTGTATGTGTAAGCACAACACAGCAGGCAGCCACT
GCCAGCACTGTGCACCATTGTACAATGACCGGCCCTGGGAGGCAGCAGATGGCAGAACAGGGGCTCCTAA
CGAATGCAGAACTTGCAAGTGCAATGGGCACGCGGACACCTGTCACTTCGACGTCAACGTGTGGGAGGCG
TCGGGGAACCGCAGCGGCGGTGTCTGCAACAACTGTCAGCACAACACTGAGGGTCAGCACTGTCAGCGCT
GTAAGCCCGGTTTCTACCGCGACCTCAGAAGACCCTTCTCCGCCCCTGACGCTTGCAAAGCGTGTTCCTG
CCACCCGGTTGGATCAGCGATCCTTCCTTTCAGCTCAGTGACCTTCTGCGACCCCAGCAATGGTGACTGC
CCCTGCAAGCCTGGGGTGGCGGGGCCACATTGTGACAGATGCATGGTGGGATACTGGGGTTTTGGAGACT
ACGGCTGCAGACCTTGCGATTGTGCGGGGAGCTGCGACCCGCTCACGGGAGACTGCATCAGCAGTAACGC
TGATGTAGACTGGTACCACGAAGTCCCCACCTTTCACTCGATGCACAATAAGAGTGAGCCCAGCTGGGAA
TGGGAGGATGAGCAAGGATTTTCTGCCCTCCGACACTCAGGTAAATGTGAATGTAAGGAACAGGTGTTAG
GAAACCCCAAAGCCTTCTGTGGAATGAAGTATTCATATGTGTTAAAAATCAAGATCTTATCAGCCCATGA
CAAAGGCTCCCATGCCGAAGTCAATGTGAAGATTAAGAAAGTCTTAAAGTCCACCAAACTGAAGATCTTA
CGAGGCAAGAGAACGCTATACCCAGAGTCCTGGACTAACAGAGGCTGCACCTGTCCAATCCTCAATCCAG
GATTGGAGTACCTGGTCGCCGGCCACGAGGACGTAAGAACGGGCAAATTAATTGTGAATATGAAAAGCTT
TGTCCAGCACTGGAAACCAGCTCTTGGCAGAAGAGTCATGCACATCTTAAAAAGAGACTGCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225293 protein sequence
Red=Cloning site Green=Tags(s)

MGSCARLLLLWGCSAVAAGLNGVAGANSRCEKACNPRMGNLALGRKLRADTMCGQNATELFCFYSENADL
TCRQPKCDKCNAAHSHLAHPPSAMADSSFRFPRTWWQSAEDVHREKIQLDLEAEFYFTHLIMVFKSPRPA
AMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAICTSRYSNPFPCTGGEVIFRALSPPYDIENP
YSAKVQEQLKITNLRVRLLKRQSCPCQINDLNAKPHHFMHYAVYDFIVKGSCFCNGHADQCLPVEGFRPI
KAPGAFHVVHGRCMCKHNTAGSHCQHCAPLYNDRPWEAADGRTGAPNECRTCKCNGHADTCHFDVNVWEA
SGNRSGGVCNNCQHNTEGQHCQRCKPGFYRDLRRPFSAPDACKACSCHPVGSAILPFSSVTFCDPSNGDC
PCKPGVAGPHCDRCMVGYWGFGDYGCRPCDCAGSCDPLTGDCISSNADVDWYHEVPTFHSMHNKSEPSWE
WEDEQGFSALRHSGKCECKEQVLGNPKAFCGMKYSYVLKIKILSAHDKGSHAEVNVKIKKVLKSTKLKIL
RGKRTLYPESWTNRGCTCPILNPGLEYLVAGHEDVRTGKLIVNMKSFVQHWKPALGRRVMHILKRDCV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021320
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021320.3, NP_067295.2
RefSeq Size 2411 bp
RefSeq ORF 1887 bp
Locus ID 57764
UniProt ID Q9JI33
Cytogenetics 10 C2
MW 69.9 kDa
Summary May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021320" in other vectors (4)
SKU Description Size Price
MC219828 Ntn4 (untagged) - Mouse netrin 4 (Ntn4), (10ug) 10 ug
$644.00
MG225293 Ntn4 (tGFP-tagged) - Mouse netrin 4 (Ntn4), (10ug) 10 ug
$785.00
MR225293L3 Lenti ORF clone of Ntn4 (Myc-DDK-tagged) - Mouse netrin 4 (Ntn4) 10 ug
$885.00
MR225293L4 Lenti ORF clone of Ntn4 (mGFP-tagged) - Mouse netrin 4 (Ntn4) 10 ug
$885.00

Plasmid Map for MR225293

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225293
Circular map for MR225293

file-alt Citations

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