Cldn20 (NM_001101560) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR225233
Cldn20 (Myc-DDK-tagged) - Mouse claudin 20 (Cldn20)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cldn20
Synonyms EG621628
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225233 representing NM_001101560
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGGCAGGTCTCCAGCTCCTTGCTTTCATCCTGGCCGTGTCTGGGGTGTCTGGAGTACTCGCTG
CCACTCTGCTGCCAAACTGGAAGGTTAATGCATACGCAGGACCCAACATTGTAACTGCTGTTGTCCAGGT
GCAGGGGTTGTGGGTGGACTGCACGTGGTACAGCACTGGGATGTTTAGCTGCACTCTAAAATACTCCATT
CTGTCGCTCCCCGTCCATGTGCAGACTGCGAGGGCCACCATGGTCCTGGCCTGTGTTCTGTCTGCTTGGG
GGATTTGTACTTCGATAGCAGGAATGAACTGCACTCACTTAGGAGGAGACACACACACCAAGAGTAAAAT
TTCCTTTGCTGGAGGAGTCTGCTTCATCACTGCAGGGATCTCTGGTTTAATCCCAACAGTGTGGTACACC
AAGGAGATCATAGCGAACTTTCTGGATCTGACCATTCCGGAAAGCCACAAATATGAACCTGGAGGAGCCC
TGTACATTGGGTTTATTTCGGCGATGCTACTACTTATCTCTGGTGTTATTTTCTGCACTTCTTATATACA
GAAGAACCAAGAACCATGGATCTACCCTCCCAAGCAGAAACTTTCCACTACCTGGCAGCCAAAGAACAGG
CGAGCACATAACTTGAAGGATTACATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR225233 representing NM_001101560
Red=Cloning site Green=Tags(s)

MASAGLQLLAFILAVSGVSGVLAATLLPNWKVNAYAGPNIVTAVVQVQGLWVDCTWYSTGMFSCTLKYSI
LSLPVHVQTARATMVLACVLSAWGICTSIAGMNCTHLGGDTHTKSKISFAGGVCFITAGISGLIPTVWYT
KEIIANFLDLTIPESHKYEPGGALYIGFISAMLLLISGVIFCTSYIQKNQEPWIYPPKQKLSTTWQPKNR
RAHNLKDYM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001101560
ORF Size 657 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001101560.1, NP_001095030.1
RefSeq Size 660 bp
RefSeq ORF 660 bp
Locus ID 621628
Cytogenetics 17 A1
MW 24.2 kDa
Summary This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is identified in retinal pigment epithelium (RPE) and analysis of the RPE transcriptome reveals that this gene expression appears late during development of chick embryo. provided by RefSeq, Aug 2010
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Documents
Resources
"NM_001101560" in other vectors (4)
SKU Description Size Price
MC215202 Cldn20 (untagged) - Mouse claudin 20 (Cldn20), (10ug) 10 ug
$330.00
MG225233 Cldn20 (tGFP-tagged) - Mouse claudin 20 (Cldn20), (10ug) 10 ug
$500.00
MR225233L3 Lenti ORF clone of Cldn20 (Myc-DDK-tagged) - Mouse claudin 20 (Cldn20) 10 ug
$600.00
MR225233L4 Lenti ORF clone of Cldn20 (mGFP-tagged) - Mouse claudin 20 (Cldn20) 10 ug
$600.00

Plasmid Map for MR225233

Download sequence for

Cloning scheme for MR225233

Circular map for MR225233

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