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Pde5a (NM_153422) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225179
Pde5a (Myc-DDK-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $792.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde5a
Synonyms Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225179 representing NM_153422
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACGAGCGGGCCCCAACTCCGTGCGGTCGCAGCAGCAGCGGGACCCGGACTGGGTGGAAGCGTGGC
TGGATGATCACCGGGACTTTACCTTCTCTTACTTTATTAGAAAGGCCACCAGAGACATGGTCAACGCATG
GTTTTCAGAGAGAGTTCACAACATCCCTGTGTGCAAGGAAGGCATCCGAGCCCATACCGAGTCCTGCTCT
TGCTCTCTGCAGCAAAGTCCTCATGCAGATAATACCACCCCTGGAGCACCAGCCAGAAAAATATCTGCCT
CTGAATTTGACCGACCTCTTAGACCCATTGTTGTCAAGGATTCCGAGGGAACAGTGAGCTTTCTCTCTGA
CTCAGGAAAAAAGGAACAAATGCCACTAACCCCCCCAAGATTTGATAGTGATGAAGGGGACCAGTGCTCA
AGGCTCTTGGAGCTAGTAAAAGATATTTCCAGTCATTTGGATGTCACAGCATTGTGTCACAAAATTTTCT
TGCATATCCATGGACTCATCTCTGCGGATCGCTATTCCCTGTTCCTTGTCTGTGAGGACAGCTCTAAAGA
CAAATTCCTCATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACATTGGAGGAAGCTTCAAATAACTGT
ATCCGTTTAGAGTGGAACAAAGGCATTGTGGGACATGTGGCAGCTTTTGGCGAGCCCTTGAACATCAAAG
ATGCCTATGAGGATCCCCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCT
TTGTATGCCAATTAAGAACCACAGGGAAGAGGTTGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGA
AATGGTGGGACCTTCACTGAAAAAGATGAAAAGGACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTC
TTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAGGTGTTACTTGACCTTGC
CAGCTTAATCTTTGAAGAGCAGCAGTCACTGGAAGTCATTCTGAAGAAGATAGCGGCCACTATCATCTCC
TTCATGCAGGTGCAGAAGTGCACCATCTTCATTGTGGATGAAGACTGTCCTGACTCTTTCTCTCGTGTGT
TTCACATGGAGTGTGAGGAAGTAGGAAAACCCTCTGATCCTTTAACGAGGGAGCAAGATGCAAACAAAAT
CAACTACATGTATGCTCAGTATGTCAAAAACACGATGGAGCCACTTAATATCCCAGATGTCACCAAGGAC
AAAAGATTTCCCTGGACAAATGAAAACATGGGACACGTGAACACGCCCTGCATTGGAAGTTTGCTTTGCA
CCCCTATAAAAAATGGGAAGAAGAACAAAGTCATAGGCGTCTGCCAACTTGTTAATAAGATGGAGGAGAA
TACTGGCAAGATTAAAGCTTTCAACCAAAATGATGAGCAGTTCCTGGAAGCCTTTGTCATCTTCTGTGGC
TTGGGGATCCAGAACACACAGATGTATGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACATTAG
AGGTCCTGTCTTACCATGCATCAGCGGCGGAGGAAGAAACAAGAGAGCTCCAGGCTTTATCGGCTGCTGT
GGTACCATCGGCCCAAACCCTTAAAATTACCGACTTCAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAA
ACAGCGCTGTGTACAATTCGGATGTTCACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGG
TTCTTTGCAGGTGGATTTTAAGTGTGAAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCA
TGCCTTTAACACGGCACAGTGCATGTTTGCTGCTCTGAAAGCCGGCAAGATCCAGAACAAGTTGACGGAT
CTGGAGACCCTTGCGTTGCTCATTGCTGCTTTAAGCCATGACTTGGATCATCGTGGCGTGAACAACTCAT
ACATACAGCGGAGCGAGCACCCGCTGGCTCAGCTGTACTGTCACTCCATCATGGAACACCATCATTTTGA
CCAGTGCTTGATGATTCTAAACAGCCCAGGCAACCAGATTCTCAGTGGCCTCTCCATTGACGAATATAAG
ACCACACTGAAAATAATCAAGCAAGCAATTTTAGCCACAGACCTAGCACTGTACATTAAGAGACGAGGAG
AATTTTTTGAACTTATACGAAAAAATCAATTCAGTTTTGAAGATCCTCTTCAAAAGGAGTTGTTTCTAGC
AATGCTGATGACAGCCTGCGACCTCTCTGCGATTACTAAACCCTGGCCTATTCAACAACGGATAGCAGAA
CTCGTGGCAGCTGAATTCTTTGATCAAGGAGACAGAGAGAGAAAAGAACTTAACATGGAGCCGGCTGATC
TAATGAACAGAGAGAAGAAAAATAAAATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCA
GCTGTATGAGGCCCTGACCCACGTATCCGAAGACTGTTTGCCCTTGCTGGATGGCTGCAGGAAGAACAGA
CAGAAATGGCAGGCCCTCGCGGAGCAGCAGGAGAAGATGCTCCTTAACGGGGAGAGCAGCCAGGGCAAGC
GGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225179 representing NM_153422
Red=Cloning site Green=Tags(s)

MERAGPNSVRSQQQRDPDWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCS
CSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCS
RLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNC
IRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSG
NGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIIS
FMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKD
KRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCG
LGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLE
TALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTD
LETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYK
TTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAE
LVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNR
QKWQALAEQQEKMLLNGESSQGKRD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153422
ORF Size 2595 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153422.2, NP_700471.2
RefSeq Size 6728 bp
RefSeq ORF 2598 bp
Locus ID 242202
UniProt ID Q8CG03
Cytogenetics 3 G1
MW 98.9 kDa
Summary Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153422" in other vectors (4)
SKU Description Size Price
MC222287 Pde5a (untagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a), (10ug) 10 ug
$872.00
MG225179 Pde5a (tGFP-tagged) - Mouse phosphodiesterase 5A cGMP-specific (Pde5a), (10ug) 10 ug
$992.00
MR225179L3 Lenti ORF clone of Pde5a (Myc-DDK-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a) 10 ug
$1,092.00
MR225179L4 Lenti ORF clone of Pde5a (mGFP-tagged) - Mouse phosphodiesterase 5A, cGMP-specific (Pde5a) 10 ug
$1,092.00

Plasmid Map for MR225179

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225179
Circular map for MR225179

file-alt Citations

Phosphodiesterase 5 expression in photoreceptors rescues retinal degeneration induced by deregulation of membrane guanylyl cyclase
Dizhoor, Sato, Luo et al
J Biol Chem (2025)
Cited by 4
AI Summary: In this study, OriGene’s PDE5 cDNA clone (cat# MR225179) was used to generate recombinant PDE5 for ectopic expression in mouse photoreceptors, enabling the investigation of its potential to rescue retinal degeneration.
Snippet: as purchased from Origene (cat#MR225179). A synthetic (Integrated DNA Technologies) DNA fragment coding for two FLAG epitopes and eight C-terminal residues of a human PDE6C al
Myoglobin maturation is driven by the hsp90 chaperone machinery and by soluble guanylyl cyclase
Ghosh, Dai, Biswas et al
FASEB J. (2019)
AI Summary: OriGene products contributed to this study by providing mouse phosphodiesterase 5A (PDE5A) and human skeletal muscle myoglobin expression constructs, which were used to investigate the molecular mechanisms underlying myoglobin maturation.
Snippet: Athens University, Ath- ens, Greece). Mouse phosphodiesterase 5A (PDE5A) and human skeletal muscle myoglobin expression constructs were purchased from OriGene (Rockville, MD, USA). Small interfering RNA (siRNA) specific
Showing 1-2 of 2 papers.
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