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Ankrd23 (NM_153502) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225070
Ankrd23 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 23 (Ankrd23)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd23
Synonyms 1110058D09Rik; 2310041C05Rik; AI853437; Darp; MARP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225070 representing NM_153502
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTCATCAGCATTGAGCAGTTGGTAAGTGGAGAAAGAGTTGATAGGAAAGCGTTGGAGTTTGGAC
GTGGAGTTCCTGATCCTGGAGGCTGGCCTAGTGGCTGGACGCTGGGACCCCAAGAGGCTGTGGCCCGGGA
GAAACTAAAGTTGGAGGAAGAGAAAAGGAAGAAACTGGAAAGATTTAACAGCTCCAGACTGACTCTGGAC
AATCTGACAGACTTGGAAAACTTGGTTCAAAGACGAAGAAAAAAACGACAGAGACACAAAGTCCCCCCCA
GGGAACCTGAGTCTGGGGCTGAGCCTCAGCCCCAGGTCCCACTGGAGCCCGTGGGCCTGGAAATGTTCCT
GAAGGCAGCTGCTGAGAACCAGGAGGCCCTGATTGACAAGTACCTGGCGGACGGAGGGGACCCCAATGCC
CATGACAAGCTCCACCGCACAGCCTTGCACTGGGCCTGTCTGAAGGGCCACAGACAGCTGGTGAACAAGC
TGCTGGCGGCAGGAGCTGCTATAGAAGTACGGGATTTGCTGGACAGGACGCCCGTGTTCTGGGCCTGCCG
CGGAGGACACCTGGACATTCTCAAACGGCTGCTTAACCAGGGAGCTCAGGTCAACGCACAAGACAAGATC
TGGAGCACCCCTCTCCATGTGGCAGTGCGCATGGGCCACTCCGACTGCCTAGAGCACCTTATCGAGTGTG
GCGCCCACATCAACGCACAGGATAAGGAAGGGGACACAGCACTCCACGAGGCCGTGCGCTATGGGCACCA
CAAAGCAACGAAACTGCTGCTGCTCTATGGAGCCAAGCTGGGTGTGAAGAATGTGGCTTCCCAGACACCA
GTGCAGCTGGCTCGAGACTGGCAGCGGGGCATCCGGGACGCACTCCAGGCCCACGTGGGCCATCCCCGTA
CCCGGTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225070 representing NM_153502
Red=Cloning site Green=Tags(s)

MDFISIEQLVSGERVDRKALEFGRGVPDPGGWPSGWTLGPQEAVAREKLKLEEEKRKKLERFNSSRLTLD
NLTDLENLVQRRRKKRQRHKVPPREPESGAEPQPQVPLEPVGLEMFLKAAAENQEALIDKYLADGGDPNA
HDKLHRTALHWACLKGHRQLVNKLLAAGAAIEVRDLLDRTPVFWACRGGHLDILKRLLNQGAQVNAQDKI
WSTPLHVAVRMGHSDCLEHLIECGAHINAQDKEGDTALHEAVRYGHHKATKLLLLYGAKLGVKNVASQTP
VQLARDWQRGIRDALQAHVGHPRTRC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153502
ORF Size 918 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153502.4, NP_705722.2
RefSeq Size 2319 bp
RefSeq ORF 921 bp
Locus ID 78321
UniProt ID Q812A3
Cytogenetics 1 B
MW 34.3 kDa
Summary May be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression.UniProtKB/Swiss-Prot Function
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"NM_153502" in other vectors (4)
SKU Description Size Price
MC211867 Ankrd23 (untagged) - Mouse ankyrin repeat domain 23 (Ankrd23), (10ug) 10 ug
$330.00
MG225070 Ankrd23 (tGFP-tagged) - Mouse ankyrin repeat domain 23 (Ankrd23), (10ug) 10 ug
$500.00
MR225070L3 Lenti ORF clone of Ankrd23 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 23 (Ankrd23) 10 ug
$600.00
MR225070L4 Lenti ORF clone of Ankrd23 (mGFP-tagged) - Mouse ankyrin repeat domain 23 (Ankrd23) 10 ug
$600.00

Plasmid Map for MR225070

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225070
Circular map for MR225070

file-alt Citations

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