Pou2f1 (NM_198933) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR225020L4
Lenti ORF clone of Pou2f1 (mGFP-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 3
  $994.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pou2f1
Synonyms 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR225020).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198933
ORF Size 2274 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198933.3, NP_945151.2
RefSeq Size 2612 bp
RefSeq ORF 2277 bp
Locus ID 18986
UniProt ID P25425
Cytogenetics 1 73.21 cM
Summary Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198933" in other vectors (4)
SKU Description Size Price
MC221373 Pou2f1 (untagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 3, (10ug) 10 ug
$764.00
MG225020 Pou2f1 (tGFP-tagged) - Mouse POU domain class 2 transcription factor 1 (Pou2f1) transcript variant 3, (10ug) 10 ug
$894.00
MR225020 Pou2f1 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 3 10 ug
$589.00 $694.00
MR225020L3 Lenti ORF clone of Pou2f1 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 3 10 ug
$994.00

Plasmid Map for MR225020L4

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Cloning scheme for MR225020L4

Circular map for MR225020L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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