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Bbs9 (NM_181316) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224929
Bbs9 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $806.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Bbs9 or NM_181316"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bbs9
Synonyms E130103I17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224929 representing NM_181316
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCT
GCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTGGGCAGCTTCATGGG
CTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTA
GAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGGGCACCGAGATGCTTC
ATCTGGCCGTGCTGCACTCCCGGAAGCTCTGTGTCTACTCCGTCTCAGGGACATTGGGAAATGTGGAACA
TGGAAACCAATATCAGATAAAACTAATGTATGAGCACCATCTTCAGAGGACGGCCTGCAATATGACGTAT
GGACCCTTTGGTGGTGTAAAAGGTCGGGATTTAATTTGTATCCAGTCTCTGGATGGGATGCTGATGGTGT
TTGAGCAGGAAAGCTATGCGTTTGGGAGGTTTCTCCCTGGTTTTCTTCTGCCTGGCCCTCTTGCTTACAG
TCCCCGCACGGATTCCTTCATAACTGTGTCTTCTTGCAGACAAGTGGAAAGTTACAAGTACCAGGTACTT
GCCTTTGCCACAGATGCAGACAAGAAGCAGGAGATGGAACAGCAAAAACTTGGTTCTGGAAAGCGGCTAG
TTGTGGACTGGACTCTGAATATTGGCGAGCAAGCCCTTGATATCTGTATCTTCCCTTTGAACCAATCTGC
ATCTTCTGTTTTTGTTCTTGGTGAGAGAAACTTCTTTTGCCTAAAGGATAATGGGCAGATTCGATTCATG
AAGAAGCTCGGTTATAACCCCAGTTGTTTTCTACCGTACTGCTCAGTTTCTGAAGGTACAATAAATACTT
TGATTGGAAACCATAACCACATGCTGCATATTTACCAGGACGTGACACTGAAGTGGGCCACACAACTCCC
CCACGTCCCCGTGGCCGTCAGAGTAGGCTGTTTTCATGATTTGAAGGGAGTGATAGTGACTCTCAGTGAT
GACGGTCACCTGCAGTGCTCGTATCTGGGGACAGACCCTTCTTTGTTCCAGGCTCCAAAAGTTGAATCAA
GAGAGCTCAACTATGATGAACTTGATGTGGAATTGAAAGAACTTCAGAAAATCATCAAAGATGTAAAGTT
GCAAGGTGTTTGGCCCTTGACTGAACAGGAAGATGACTTGAAAGTCTCTGCTTCAGTGTCTTCTACCCTG
GATTCTGTGTCTCAAGCCACCAATGTGGAACCTGGAGCTGACTCTGTCCCGTCCATCACAGTGAAGATTA
CCCTGCAGAACCGAGTGGTGTTGCAGAAGGTCAAGCTGTCTATCTATGTGCAGCCTCCACTACAGTTGAC
TTGTGATCAGTTCACCTTTGACTTCACGGTTCCAGATATGACGAGTTCAGTAGCGTTTTCTGTGTACCTG
AAGAGAAACTACACACCATCCGAGTTAGAAGGAAATGCTGTTGTTTCTTACTCCAGACCGACAGGTATTC
CTCGGGTTGTCCAATGTAAATTTAGACTTCCTCTGAAACTGATTTGCCTACCTGGTCAGCCTTCAAAAAC
TGCAAGCCACAAACTAACTATAGACACCAACAAATCTCCTGTCAGTCTGCTCGGTCTCTTCCCAGACTTT
GCCAACCCATCAGATGACGACCAAGTGAATGTAATGGGCTTCCGTCTCCTAGGAGGCGCTCGAGTGACTC
TTCTTGCTTCCAGAACATCCCAACGATACCGCATTCAGAGCGAACAGTTTGAAGATCTCTGGCTCATAAC
CAATGAACTCATCCTCCGCCTTCAGGAACATTTTGAAAAACAGGGAACCAAAGACTTTTCGTGTTCTTTT
TCTGGGTGTGTGCCCCTTCAAGAATATTTTGAGTTGATTGATCATCATTTTGAGCTGCGGATAAATGGTA
AAAAATTGGAAGAACTTTTATCTGAAAGAGCTGTACAATTTCGGGCCATTCAGCGTCGGCTTCTGACAAG
ATTCAGAGACAAGACCCCTGCCCCACTGCAGCACCTGGACACCCTGCTGGACGGGACATACAAGCAGGTG
ATTGCTCTAGCAGATGCGATCGAGGAGAACCAGGACAGGCTGTTGCAGTCATTCTCAGGCCTGAAGAGTG
CCACCCATTTGCTGATCCTGCTAATCAGGCTGTGGCAGAGGCTGAGCGCTGACCAGACTGCTATTCTGGA
AGCAGCATTTCTGCCACTACAGGAAGACACGCAGGAACTGGGCTGGGAAGAAACCGTGGATGCTGCCATT
GCCTACCTTTTGAAAACCTGCCTGTCGAAGAGTTCCAAGGAGCAGGCTTTGAACCTCAGCAGCCAACTGA
ACATACCCAAAGATACCAGCCGGCTGAAGAAGCACATCACCCTGCTGTGTGATAGGTTAGCCAAAGGTGG
GCGTCTCTGCGTGAGCACAGATGCAGCGGCCCCGCAGGCCATGGTTGTGCCAGGTGGCTGTACTCCAATC
CCAGAGTCAGACCTAGAGGAAAGGTCACTAGATGACTCCACAGAGCTGTTTACCAACCACAAGCACCTCA
TGACCGAGCCCCCCATGCCTGAAGTCTCAGCCCGCCAAGGAGTTTTGGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224929 representing NM_181316
Red=Cloning site Green=Tags(s)

MSLFKARDWWSTVLGEKEEFDQGCLCLADVDNSGNGHDKIIVGSFMGYLRIFSPHSVKAGGGPQAEDLLL
EVHLRDPVLQVEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQYQIKLMYEHHLQRTACNMTY
GPFGGVKGRDLICIQSLDGMLMVFEQESYAFGRFLPGFLLPGPLAYSPRTDSFITVSSCRQVESYKYQVL
AFATDADKKQEMEQQKLGSGKRLVVDWTLNIGEQALDICIFPLNQSASSVFVLGERNFFCLKDNGQIRFM
KKLGYNPSCFLPYCSVSEGTINTLIGNHNHMLHIYQDVTLKWATQLPHVPVAVRVGCFHDLKGVIVTLSD
DGHLQCSYLGTDPSLFQAPKVESRELNYDELDVELKELQKIIKDVKLQGVWPLTEQEDDLKVSASVSSTL
DSVSQATNVEPGADSVPSITVKITLQNRVVLQKVKLSIYVQPPLQLTCDQFTFDFTVPDMTSSVAFSVYL
KRNYTPSELEGNAVVSYSRPTGIPRVVQCKFRLPLKLICLPGQPSKTASHKLTIDTNKSPVSLLGLFPDF
ANPSDDDQVNVMGFRLLGGARVTLLASRTSQRYRIQSEQFEDLWLITNELILRLQEHFEKQGTKDFSCSF
SGCVPLQEYFELIDHHFELRINGKKLEELLSERAVQFRAIQRRLLTRFRDKTPAPLQHLDTLLDGTYKQV
IALADAIEENQDRLLQSFSGLKSATHLLILLIRLWQRLSADQTAILEAAFLPLQEDTQELGWEETVDAAI
AYLLKTCLSKSSKEQALNLSSQLNIPKDTSRLKKHITLLCDRLAKGGRLCVSTDAAAPQAMVVPGGCTPI
PESDLEERSLDDSTELFTNHKHLMTEPPMPEVSARQGVLE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181316
ORF Size 2640 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181316.4, NP_851833.2
RefSeq Size 3382 bp
RefSeq ORF 2643 bp
Locus ID 319845
UniProt ID Q811G0
Cytogenetics 9 A3
MW 98.5 kDa
Summary The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181316" in other vectors (4)
SKU Description Size Price
MC222404 Bbs9 (untagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9), transcript variant 2, (10ug) 10 ug
$887.00
MG224929 Bbs9 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9) transcript variant 2, (10ug) 10 ug
$1,006.00
MR224929L3 Lenti ORF clone of Bbs9 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9), transcript variant 2 10 ug
$1,106.00
MR224929L4 Lenti ORF clone of Bbs9 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 9 (human) (Bbs9), transcript variant 2 10 ug
$1,106.00

Plasmid Map for MR224929

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224929
Circular map for MR224929

file-alt Citations

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