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Arsj (NM_173451) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224832
Arsj (Myc-DDK-tagged) - Mouse arylsulfatase J (Arsj)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $558.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arsj
Synonyms 9330196J05Rik; D830047F08
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCAGGGACAGTGCGGAGCCTCTGCCTCCGCTATCTCCACAGGCCTGGGCCTGGTCTGGAAAGT
TCTTAGCGATGGGTGCCCTGGCAGGCTTCTCGGTCCTCAGCCTGCTCACCTATGGCTACCTGTGCTGGGG
CCAGGACTTAGAAGAGGAGGGGTCCCTAAAAGCTCAAGTTGATGAGAGACCTGAAGCAGGCACAGCAGGC
ACCTCCCAGCCCCATCTCATTTTTATCTTAGCTGATGATCAGGGATTTAGAGATGTGGGTTACCACGGAT
CTGAGATAAAGACGCCCACCCTTGATAAACTCGCTGCAGAAGGAGTTAAGCTAGAGAACTACTATGTCCA
GCCTATTTGCACACCGTCCAGGAGCCAGTTTATTACCGGAAAGTATCAGATACACACGGGACTTCAGCAT
TCTATCATTAGACCTACCCAACCCAACTGTCTACCTCTGGACAATGCAACCCTACCTCAGAAGCTAAAGG
AGGTCGGCTATTCAACCCATATGGTTGGGAAGTGGCACTTGGGATTTTACAGAAAAGACTGCATGCCCAC
CAAGAGAGGATTTGATACCTTTTTTGGCTCTCTTTTGGGAAGTGGTGATTATTATACACACTACAAATGT
GACAGTCCTGGGGTGTGTGGCTATGACTTGTACGAAAACGACAATGCAGCTTGGGACTATGACAATGGCA
TCTATTCAACACAGATGTACACGCAGAGAGTGCAGCAAATCTTAGCCACCCATGATCCCACAAAACCTCT
ATTTTTATATGTGGCTTACCAAGCTGTCCACTCACCACTTCAAGCCCCTGGCAGGTACTTTGAACACTAC
AGATCCATTATCAACATAAACAGGAGGAGGTACGCAGCCATGCTCTCCTGCTTGGATGAAGCAATCCACA
ATGTGACCCTGGCCCTAAAGAGGTATGGTTTCTATAACAATAGCATTATCATTTACTCCTCAGATAATGG
TGGGCAGCCCACAGCAGGAGGAAGTAACTGGCCACTCAGAGGCAGCAAAGGAACATATTGGGAAGGAGGC
ATCCGGGCAGTTGGCTTTGTGCATAGCCCACTTCTAAAAAACAAGGGAACGGTATGTAAGGAACTTGTGC
ACATCACCGATTGGTACCCAACCTTGATTTCACTGGCTGAAGGACAGATTGATGAAGACATTCAGCTAGA
TGGATACGATATCTGGGAGACCATAAGCGAAGGTCTTCGTTCACCCCGAGTGGATATTTTGCACAACATT
GACCCCATTTACACCAAGGCGAAAAATGGCTCCTGGGCAGCAGGCTATGGGATCTGGAACACTGCAATCC
AGTCGGCCATCCGGGTACAACACTGGAAACTGCTCACAGGCAATCCTGGCTACAGTGACTGGGTCCCCCC
TCAGGCTTTCAGCAATCTGGGCCCAAACCGGTGGCACAATGAAAGGATTACCTTGTCAACTGGCAAGAGT
ATCTGGCTTTTCAACATCACGGCTGATCCGTATGAGAGGGTGGACCTATCCAGCAGGTATCCAGGCATCG
TGAAGAAGCTGCTGCGGAGACTGTCACAATTCAACAAGACTGCCGTGCCTGTCAGGTACCCCCCGAAGGA
TCCCAGAAGCAACCCTAGGCTCAACGGAGGAGTCTGGGGTCCGTGGTATAAAGAGGAAAACAAGAAAAAG
AAGTCAAACAAAACCAAGGCTAAGAAAATGCAAAAGAAAAAAAGTAAGGCAAGGATGAGGAAGCAGTTGG
CAGCTCACTCCTCAATAAAATGCCACCCAAGTGTTGCCACTGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224832 protein sequence
Red=Cloning site Green=Tags(s)

MAPRDSAEPLPPLSPQAWAWSGKFLAMGALAGFSVLSLLTYGYLCWGQDLEEEGSLKAQVDERPEAGTAG
TSQPHLIFILADDQGFRDVGYHGSEIKTPTLDKLAAEGVKLENYYVQPICTPSRSQFITGKYQIHTGLQH
SIIRPTQPNCLPLDNATLPQKLKEVGYSTHMVGKWHLGFYRKDCMPTKRGFDTFFGSLLGSGDYYTHYKC
DSPGVCGYDLYENDNAAWDYDNGIYSTQMYTQRVQQILATHDPTKPLFLYVAYQAVHSPLQAPGRYFEHY
RSIININRRRYAAMLSCLDEAIHNVTLALKRYGFYNNSIIIYSSDNGGQPTAGGSNWPLRGSKGTYWEGG
IRAVGFVHSPLLKNKGTVCKELVHITDWYPTLISLAEGQIDEDIQLDGYDIWETISEGLRSPRVDILHNI
DPIYTKAKNGSWAAGYGIWNTAIQSAIRVQHWKLLTGNPGYSDWVPPQAFSNLGPNRWHNERITLSTGKS
IWLFNITADPYERVDLSSRYPGIVKKLLRRLSQFNKTAVPVRYPPKDPRSNPRLNGGVWGPWYKEENKKK
KSNKTKAKKMQKKKSKARMRKQLAAHSSIKCHPSVATG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173451
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173451.3, NP_775627.1
RefSeq Size 3690 bp
RefSeq ORF 1797 bp
Locus ID 271970
UniProt ID Q8BM89
Cytogenetics 3 G1
MW 67.4 kDa
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Documents
Resources
"NM_173451" in other vectors (4)
SKU Description Size Price
MC219585 Arsj (untagged) - Mouse arylsulfatase J (Arsj), (10ug) 10 ug
$613.00
MG224832 Arsj (tGFP-tagged) - Mouse arylsulfatase J (Arsj), (10ug) 10 ug
$758.00
MR224832L3 Lenti ORF clone of Arsj (Myc-DDK-tagged) - Mouse arylsulfatase J (Arsj) 10 ug
$858.00
MR224832L4 Lenti ORF clone of Arsj (mGFP-tagged) - Mouse arylsulfatase J (Arsj) 10 ug
$858.00

Plasmid Map for MR224832

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224832
Circular map for MR224832

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