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Trim71 (NM_001042503) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224725
Trim71 (Myc-DDK-tagged) - Mouse tripartite motif-containing 71 (Trim71)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $783.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim71
Synonyms 636931; 2610206G21Rik; AL022943; Gm1127; lin-41; Lin41; mlin-41; mLin41
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224725 representing NM_001042503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGTTCCCGGAGACCGACTTCCAGATCTGCCTGCTGTGCAAGGAGATGTGCGGCTCGCCCGCGC
CGCTCTCCTCCAACTCGTCCGCCTCGTCGTCGTCCTCGCAGACTTCCACGTCGTCGGCGGGCGGCGGCGG
CCCCGGGGCGGCGGCGCGGCGTCTTCACGTCTTGCCCTGCCTGCACGCCTTCTGCCGGCCGTGCCTCGAG
GCGCACCGGCTGCCCGCTCCCGGAGGCGCGGGCCCCGCCGAGGCGCTGAAGCTGCGCTGCCCCGTATGTG
ACCAGAAGGTGGTCCTGGCCGAGGCGGCGGGCATGGACGCGCTGCCATCCTCCGCCTTCCTGCTCAGCAA
CCTGCTGGACGCCGTGGTGGCCACCGCCGAAGAGCCGCCGCCCAAGAACGGGCGCGCGGGCGGCGGCCCC
GGGGGCGCGGGCGGCCACAGCAACCACCGGCACCATGCGCACCACCCGGCCCAGCGCGCCGCCGCTCCAG
CGCCGCAGCCGCCGCCCGGCCCGGCCGCCTCCCCCGGGTCGCTGCTGATGCGCCGGCCGCACGGCTGCAG
CTCCTGCGACGAGGGCAACGCCGCCTCGTCGCGCTGCCTCGACTGCCAGGAACACCTGTGCGACAACTGT
GTCCGCGCGCACCAGCGCGTCCGCCTCACCAAGGACCATTACATCGAGCGCGGCCCTCCGGGGCCTGCGG
CCGCCTCCGCGGCACAGCAGCTGGGGCTCGGGCCGCCCTTCGCGGGCGCGCCCTTCTCCATTCTCTCGGT
GTTCCCGGAGCGCCTCGGCTTCTGCCAGCACCACGACGATGAGGTCCTGCATCTCTACTGTGACACCTGC
TCTGTCCCCATCTGCAGAGAGTGCACGCTGGGCCGCCACGGTGGCCACAGCTTCGCCTACCTCCAGGATG
CCCTGCAGGACTCTCGGGCACTCACCATCCAGCTGCTGGCTGATGCCCAGCAGGGCCGCCAGGCTCTCCA
GCTGAGCATCGAGCAGGCCCAGACTGTGGCAGAACAGGTGGAGATGAAGGCGAAGGTGGTCCAGTCTGAG
GTCAAGGCCGTCACAGCTCGCCACAAGAAGGCCCTGGAGGATCGGGAGTGTGAGCTGTTGTGGAAGGTGG
AGAAGATCCGGCAGGTGAAGGCGAAGTCTCTGTTCCTGCAGGTGGAGAAGCTGCGGCAGAGTCTCAGCAA
GCTGGAGAGCACCATCAGCGCCGTGCAGCAGGTCCTGGAAGAGGGCCGCGCCCTGGACATCCTGCTGGCC
CGAGACCGGATGCTGGCCCAGGTGCAGGAGCTCAAGACCATCCGAGGCCTCCTGCAGCCCCAAGAAGATG
ACCGCATTATGTTCACGCCCCCTGACCAGGCCCTGTACCTCGCCCTCAAGTCTATTGGCTTTGTCAGCAG
CGGGGCCTTCGCCCCGCTCACGAAGGCTACAGGAGATGGCATCAAGCGTGCCCTTCAGGGGAAGGTGGCC
TCTTTCACTGTCATGGGCTACGACCACGATGGTGAGCCCCGCCATTCGGGAGGTGACCTGATGTCAGTTG
TGGTCCTCGGCCCCGACGGAAACCTGTTTGGTGCAGAAGTGAGCGACCAGCAGAATGGTACTTACATAGT
GAGCTACAGGCCCCAGCTTGAGGGTGAGCACTTGGTGTCGGTGACATTGTACAATCAGCATATAGAGAAC
AGCCCCTTCAAGGTCGTGGTCAAGTCGGGCCGCAGCTACGTGGGCATCGGGCTCCCCGGGCTGAGCTTCG
GCAGTGAAGGTGACGGGGAGGGCAAGCTCTGCAGGCCCTGGGGCGTGAGTGTTGATAAGGAGGGTTTCAT
CATAGTGGCTGACCGAAGCAATAACAGAATCCAGGTGTTTAAGCCCTGTGGTTCCTTCCACCACAAGTTT
GGTACCCTTGGGTCTCGTCCTGGGCAGTTTGACCGACCGGCTGGGGTGGCTTGTGACGCCTCCCGCAGGA
TCATAGTGGCCGACAAAGACAATCATCGCATTCAGATCTTCACCTTCGAGGGCCAGTTCCTTCTTAAGTT
TGGTGAGAAAGGCACCAAGAACGGGCAGTTTAACTACCCGTGGGATGTAGCAGTGAATTCTGAGGGCAAG
ATCCTCGTTTCAGACACCCGGAATCATCGCATCCAGTTGTTCGGACCGGACGGGGTCTTCCTGAACAAGT
ACGGCTTCGAGGGATCTCTCTGGAAGCACTTCGACTCTCCACGGGGCGTGGCTTTCAACAATGAAGGTCA
CCTGGTAGTCACCGACTTCAACAACCACCGGCTTCTGGTTATTCACCCTGATTGCCAATCTGCCCGCTTC
TTGGGTTCGGAGGGCTCGGGCAATGGGCAGTTCCTGCGACCACAGGGTGTGGCTGTGGACCAGGAAGGGA
GAATCATCGTAGCCGATTCCAGAAATCACCGCGTGCAGATGTTTGAGGCCAATGGCAGCTTCCTGTGCAA
GTTTGGTGCTCAAGGGAGTGGCTTCGGGCAGATGGACCGCCCCTCAGGTATCGCGGTCACCCCGGATGGA
TTGATCGTCGTGGTGGACTTTGGCAACAATCGAATCCTCATCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224725 representing NM_001042503
Red=Cloning site Green=Tags(s)

MASFPETDFQICLLCKEMCGSPAPLSSNSSASSSSSQTSTSSAGGGGPGAAARRLHVLPCLHAFCRPCLE
AHRLPAPGGAGPAEALKLRCPVCDQKVVLAEAAGMDALPSSAFLLSNLLDAVVATAEEPPPKNGRAGGGP
GGAGGHSNHRHHAHHPAQRAAAPAPQPPPGPAASPGSLLMRRPHGCSSCDEGNAASSRCLDCQEHLCDNC
VRAHQRVRLTKDHYIERGPPGPAAASAAQQLGLGPPFAGAPFSILSVFPERLGFCQHHDDEVLHLYCDTC
SVPICRECTLGRHGGHSFAYLQDALQDSRALTIQLLADAQQGRQALQLSIEQAQTVAEQVEMKAKVVQSE
VKAVTARHKKALEDRECELLWKVEKIRQVKAKSLFLQVEKLRQSLSKLESTISAVQQVLEEGRALDILLA
RDRMLAQVQELKTIRGLLQPQEDDRIMFTPPDQALYLALKSIGFVSSGAFAPLTKATGDGIKRALQGKVA
SFTVMGYDHDGEPRHSGGDLMSVVVLGPDGNLFGAEVSDQQNGTYIVSYRPQLEGEHLVSVTLYNQHIEN
SPFKVVVKSGRSYVGIGLPGLSFGSEGDGEGKLCRPWGVSVDKEGFIIVADRSNNRIQVFKPCGSFHHKF
GTLGSRPGQFDRPAGVACDASRRIIVADKDNHRIQIFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGK
ILVSDTRNHRIQLFGPDGVFLNKYGFEGSLWKHFDSPRGVAFNNEGHLVVTDFNNHRLLVIHPDCQSARF
LGSEGSGNGQFLRPQGVAVDQEGRIIVADSRNHRVQMFEANGSFLCKFGAQGSGFGQMDRPSGIAVTPDG
LIVVVDFGNNRILIF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042503
ORF Size 2565 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042503.2, NP_001035968.1
RefSeq Size 5197 bp
RefSeq ORF 2568 bp
Locus ID 636931
UniProt ID Q1PSW8
Cytogenetics 9 F3
MW 92.5 kDa
Summary E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (PubMed:19898466). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression (PubMed:22735451). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (PubMed:23125361). Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (PubMed:22735451). Specific regulator of miRNA biogenesis. miRNA Binds MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001042503" in other vectors (4)
SKU Description Size Price
MC222236 Trim71 (untagged) - Mouse tripartite motif-containing 71 (Trim71), (10ug) 10 ug
$862.00
MG224725 Trim71 (tGFP-tagged) - Mouse tripartite motif-containing 71 (Trim71), (10ug) 10 ug
$983.00
MR224725L3 Lenti ORF clone of Trim71 (Myc-DDK-tagged) - Mouse tripartite motif-containing 71 (Trim71) 10 ug
$1,083.00
MR224725L4 Lenti ORF clone of Trim71 (mGFP-tagged) - Mouse tripartite motif-containing 71 (Trim71) 10 ug
$1,083.00

Plasmid Map for MR224725

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224725
Circular map for MR224725

file-alt Citations

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