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Trim50 (NM_178240) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224711
Trim50 (Myc-DDK-tagged) - Mouse tripartite motif-containing 50 (Trim50)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224711 representing NM_178240
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGGCGGCTGACTGTGCCGGAACTCCAGGACCAGCTGCAGTGTCCCATTTGCCTGGAGGTCTTCA
AAGAGCCTCTGATGCTACAGTGCGGCCACTCCTACTGCAAGGACTGCCTGGATAACTTGTCCCAACACCT
GGACTCCGAGCTATGCTGTCCTGTGTGTCGTCAGTCAGTAGACTGCAGCAGCTCCCCGCCTAATGTCTCC
CTGGCCCGGGTGATAGATGCTCTGAGGCTGCCGGGGGACATAGAGCCCACCGTCTGCGTGCACCACCGAA
ACCCTCTCAGCCTCTTCTGCGAGAAAGACCAGGAATTCATCTGTGGTCTCTGTGGCCTGCTGGGCTCCCA
CCAACACCACCGAGTCACACCAGTCTCCACCGTCTACAGCCGCATGAAGGAAGAGCTTGCAGGCCGCATA
TCTGAGCTGAAGGAGGAGCACAGGAACGTGGAGGAGCACATCGGCAAACTGGTGAACAACAGGACCCGGA
TTATTAATGAGTCCGATGTCTTCAGCTGGGTGATCCGAAGGGAGTTCCAGGAATTGCACCACCTGGTGGA
CGAAGAGAAAGCCCGCTGCCTGGAGGGGCTTGAGGGCCATACGCGTGGCCTGGTGGCCTCCCTGGACATG
CAGCTGGAGCAGGCTCAGGGTACACAAGAGCGACTGGCTCAGGCGGAGCAGGTGCTGGAGCAGTTTGGGA
ACGAGAGTCACCATGAGTTCATCCGGTTCCACTCTGTGGCCTCCAGGGCAGAGGTCCAGCAGGCTCGGCC
TTTGGAAGGCGTCTTCAGTCCCATCTCCTTCAAGCCAGCTCTCCACCAGGCTGACATCAAACTGACCGTG
TGGAAGAGACTCTTCCGGAAGGTTCTGCCAGCTCCAGCATCTCTCAAGCTGGACCCGGCTACTGCCCACC
CTCTGCTGGAGCTCTCTAAGGGCAACACAGTGGTGCATTGTGGCCTCCTTGCCCAGAGACGAGCCAGCCA
ACCCGAGCGCTTCGACTATAGCACCTGTGTCCTGGCTAGCAAGGGCTTCTCTTGGGGTCGCCACTACTGG
GAAGTGGTGGTGGGGAGCAAGAGTGACTGGCGCTTGGGGGTCATCAAGGGCACAGCCAGCCGCAAAGGCA
AGCTGAACAAGTCCCCAGAGCACGGTGTGTGGCTAATTGGCCTGAAAGAGGGTCGGGTGTATGAGGCTTT
TGGCTGCCCACGGCTGCCACTGCCTGTGGCTGGCCACCCTCATCGCATCGGCGTCTACTTACACTATGAG
CAGGGTGAGCTCACGTTCTTTGACGCTGACCGTCCTGATGATCTGCGGACACTCTATACTTTCCAAGCTG
ACTTCCAGGGCAAGCTGTACCCTATCCTGGACACATGTTGGCATGAGAGGGGCAGCAACTCACTGCCCAT
GGTATTGCCCCCACCCAGTGGGCCTGGGCACTTCACACTGGGACAGGTA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224711 representing NM_178240
Red=Cloning site Green=Tags(s)

MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVS
LARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRI
SELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDM
QLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTV
WKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYW
EVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYE
QGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_178240
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178240.3
RefSeq Size 1452 bp
RefSeq ORF 1452 bp
Locus ID 215061
UniProt ID Q810I2
Cytogenetics 5 G2
MW 55.1 kDa
Summary E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.UniProtKB/Swiss-Prot Function
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"NM_178240" in other vectors (4)
SKU Description Size Price
MC216710 Trim50 (untagged) - Mouse tripartite motif-containing 50 (Trim50), (10ug) 10 ug
$503.00
MG224711 Trim50 (tGFP-tagged) - Mouse tripartite motif-containing 50 (Trim50), (10ug) 10 ug
$657.00
MR224711L3 Lenti ORF clone of Trim50 (Myc-DDK-tagged) - Mouse tripartite motif-containing 50 (Trim50) 10 ug
$757.00
MR224711L4 Lenti ORF clone of Trim50 (mGFP-tagged) - Mouse tripartite motif-containing 50 (Trim50) 10 ug
$757.00

Plasmid Map for MR224711

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224711
Circular map for MR224711

file-alt Citations

TRIM50 suppressed hepatocarcinoma progression through directly targeting SNAIL for ubiquitous degradation
Ma, Ma, Qiu et al
Cell Death Dis (2018) 9 (6), 608 DOI: 10.1038/s41419-018-0644-4
AI Summary: OriGene contributed to this study by providing the TRIM50 plasmid, which was used for exogenous overexpression experiments to investigate the tumor-suppressive role of TRIM50 in hepatocellular carcinoma.
Snippet: … TRIM50 plasmid was synthesized by OriGene (OriGene Technologies, Maryland, USA). RING domain deleted mutant of TRIM50 was generated using the KOD-Plus-Mutagenesis kit (Toyobo, Osaka, Japan) according to the manufacture’s protocol. …TRIM50 plasmid was synthesized by OriGene (OriGene Technologies, Maryland, USA). RING domain deleted mutant of TRIM50 was generated using the KOD-Plus-Mutagenesis kit (Toyobo, Osaka, Japan) according to the manufacture’s protocol. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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