Mgl2 (NM_145137) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224579
Mgl2 (Myc-DDK-tagged) - Mouse macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mgl2
Synonyms CD301b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224579 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATGAGATATGAAAACTTCCAGAACTTGGAGCGGGAAGAGAAAAACCAGGAGATGAGAAATGGTG
ACAAGAAAGGAGGAATGGAGTCTCCAAAGTTTGCTCTAATTCCTTCCCAGTCCTTCCTGTGGCGCATCCT
CTCTTGGACCCACCTCCTCCTGTTCTCCCTGGGCCTCAGCCTCCTGCTACTGGTGGTCATCTCCGTGATT
GGATCCCAAAATTCCCAGTTAAGGAGGGACCTAGGCACCCTAAGAGCCATTTTAGACAACACCACCTCCA
AGATAAAGGCTGAATTCCAGTCCCTGGACTCCAGGGCTGACAACTTCGAAAAAGGGATCAGTTCTCTGAA
AGTGGATGTGGAGGATCACAGGCAGGAACTGCAGGCAGGCCGAGACTTGAGCCAGAAGGTGACTTCTCTG
GAGAGCACACTGGAGAAGAGGGAGCAGGCTCTCAAAACAGATCTGTCTGATTTAACCGACCATGTACAAC
AGCTGGAGACAGACTTGAAGGCCTTGACGTGCCAGCTGGCCAACCTCAAGAACAATGGCTCGGAAGTGGC
CTGCTGCCCGCTTCACTGGACGGAGCATGAAGGCAGCTGCTATTGGTTCTCTGAGTCTGAGAAGTCGTGG
CCTGAAGCTGACAAGTACTGCCGGCTGGAGAATTCTCACCTGGTGGTGGTCAACTCCCTGGAGGAGCAGA
ATTTTCTACAGAATCGCTTAGCCAATGTGCTTAGCTGGATGGGCCTAACGGACCAAAATGGGCCCTGGCG
ATGGGTGGATGGGACCGACTTTGACAAAGGTTTCAAGAATTGGAGGCCACTGCAGCCGGATAACTGGCAT
GGACATATGCTAGGTGGAGGCGAGGACTGTGCCCACTTTTCTTACGATGGTCGTTGGAATGACGATGTCT
GCCAGAGGCATTACCATTGGATCTGTGAAACAGAGCTGGGCAAAGCCAGCTCAGCCCACTCCCCACAACT
TATAGCCTCAGTTCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224579 protein sequence
Red=Cloning site Green=Tags(s)

MTMRYENFQNLEREEKNQEMRNGDKKGGMESPKFALIPSQSFLWRILSWTHLLLFSLGLSLLLLVVISVI
GSQNSQLRRDLGTLRAILDNTTSKIKAEFQSLDSRADNFEKGISSLKVDVEDHRQELQAGRDLSQKVTSL
ESTLEKREQALKTDLSDLTDHVQQLETDLKALTCQLANLKNNGSEVACCPLHWTEHEGSCYWFSESEKSW
PEADKYCRLENSHLVVVNSLEEQNFLQNRLANVLSWMGLTDQNGPWRWVDGTDFDKGFKNWRPLQPDNWH
GHMLGGGEDCAHFSYDGRWNDDVCQRHYHWICETELGKASSAHSPQLIASVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145137
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145137.2, NP_660119.1
RefSeq Size 1550 bp
RefSeq ORF 999 bp
Locus ID 216864
Cytogenetics 11 B3
MW 38.1 kDa
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Documents
Resources
"NM_145137" in other vectors (4)
SKU Description Size Price
MC212655 Mgl2 (untagged) - Mouse macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2), (10ug) 10 ug
$330.00
MG224579 Mgl2 (tGFP-tagged) - Mouse macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2), (10ug) 10 ug
$489.00 $500.00
MR224579L3 Lenti ORF clone of Mgl2 (Myc-DDK-tagged) - Mouse macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2) 10 ug
$600.00
MR224579L4 Lenti ORF clone of Mgl2 (mGFP-tagged) - Mouse macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2) 10 ug
$600.00

Plasmid Map for MR224579

Download sequence for

Cloning scheme for MR224579

Circular map for MR224579

file-alt Citations

CD301 mediates fusion in IL-4-driven multinucleated giant cell formation
Brooks, Wang, Magalhaes et al
J Cell Sci (2020) 133 (24) DOI: 10.1242/jcs.248864
Cited by 8
AI Summary: In this study, OriGene’s CRISPR/Cas9 knockout plasmids targeting Mgl1 and Mgl2 were used to delete the mouse CD301 genes, demonstrating that loss of CD301 impairs IL-4-induced multinucleated giant cell formation and confirming CD301’s essential role in this process.
Showing 1-1 of 1 papers.
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