Bmyc (NM_023326) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224545
Bmyc (Myc-DDK-tagged) - Mouse brain expressed myelocytomatosis oncogene (Bmyc)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bmyc
Synonyms 2900002K07Rik; AW060705; Mycb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224545 representing NM_023326
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCTCCACGTGAGCCTCGCTAACGGCAACCGCGACCTGGACTATGACTCGGTGCAGCCATACTTCA
TGTGCGACGATGAGGAGGAGGACGTGCACCACCAGCAGCCGCCGCAGCCGCCAGCGCCCAGCGAGGACAT
CTGGAAGAAATTCGAGCTGCTGCCCACGCCGCGCCCGTCCCCGGGCCACGCCGGGCTCTACTCGCCTCCC
TGCGAAGCGGTCGCCGTGTCTTTCGCCCCCAGGGACCACGACGGTGATAGCTTCTCCATCGCAGACCTGC
CCGAGCTGCCAGGAGGAGACGCGGTGAAGCAGAGCTTCGTCTGCGACCCGGATGACGAGACCTTCGTGAA
GAACATCATCCTGCAGGACTGTATGTGGAATGGCTTCTCGGCCTCCGCCAAGCTGGTCTCCAAGCTGGAC
CCCTACCAGGCTGTGCGCAAAGAAGGCACCGGCGTGAGCCTGGCCGCCGACGTGGAGCCTGCTACGCCTC
CAGACTGCACCTGCAACACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224545 representing NM_023326
Red=Cloning site Green=Tags(s)

MPLHVSLANGNRDLDYDSVQPYFMCDDEEEDVHHQQPPQPPAPSEDIWKKFELLPTPRPSPGHAGLYSPP
CEAVAVSFAPRDHDGDSFSIADLPELPGGDAVKQSFVCDPDDETFVKNIILQDCMWNGFSASAKLVSKLD
PYQAVRKEGTGVSLAADVEPATPPDCTCNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023326
ORF Size 510 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023326.2, NP_075815.2
RefSeq Size 861 bp
RefSeq ORF 513 bp
Locus ID 107771
UniProt ID Q6P8Z1
Cytogenetics 2 A3
MW 18.5 kDa
Summary Seems to act as an inhibitor of cellular proliferation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023326" in other vectors (4)
SKU Description Size Price
MC212149 Bmyc (untagged) - Mouse brain expressed myelocytomatosis oncogene (Bmyc), (10ug) 10 ug
$330.00
MG224545 Bmyc (tGFP-tagged) - Mouse brain expressed myelocytomatosis oncogene (Bmyc), (10ug) 10 ug
$500.00
MR224545L3 Lenti ORF clone of Bmyc (Myc-DDK-tagged) - Mouse brain expressed myelocytomatosis oncogene (Bmyc) 10 ug
$600.00
MR224545L4 Lenti ORF clone of Bmyc (mGFP-tagged) - Mouse brain expressed myelocytomatosis oncogene (Bmyc) 10 ug
$600.00

Plasmid Map for MR224545

Download sequence for

Cloning scheme for MR224545

Circular map for MR224545

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