Cyp19a1 (NM_007810) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR224509
Cyp19a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 19, subfamily a, polypeptide 1 (Cyp19a1)
$702.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cyp19a1 |
| Synonyms | Ar; ArKO; Cyp19; Int-5; Int5; p450arom |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007810 |
| ORF Size | 1509 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007810.4 |
| RefSeq Size | 2373 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 13075 |
| UniProt ID | P28649 |
| Cytogenetics | 9 29.49 cM |
| MW | 58.5 kDa |
| Summary | Catalyzes the formation of aromatic C18 estrogens from C19 androgens.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007810" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216951 | Cyp19a1 (untagged) - Mouse cytochrome P450, family 19, subfamily a, polypeptide 1 (Cyp19a1), (10ug) | 10 ug |
$750.00
|
|
| MG224509 | Cyp19a1 (tGFP-tagged) - Mouse cytochrome P450 family 19 subfamily a polypeptide 1 (Cyp19a1), (10ug) | 10 ug |
$902.00
|
|
| MR224509L3 | Lenti ORF clone of Cyp19a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 19, subfamily a, polypeptide 1 (Cyp19a1) | 10 ug |
$1,002.00
|
|
| MR224509L4 | Lenti ORF clone of Cyp19a1 (mGFP-tagged) - Mouse cytochrome P450, family 19, subfamily a, polypeptide 1 (Cyp19a1) | 10 ug |
$1,002.00
|
|
Citations
Brain-synthesized oestrogens regulate cortical migration in a sexually divergent manner
Sellers, Denley, Saito et al
Eur J Neurosci (2020) 52 (1), 2646-2663 DOI: 10.1111/ejn.14755
Sellers, Denley, Saito et al
Eur J Neurosci (2020) 52 (1), 2646-2663 DOI: 10.1111/ejn.14755
Cited by 15
AI Summary: In this study, OriGene's mouse Cyp19a1 shRNA plasmid (TL502431) was used to knock down aromatase expression in developing mouse cortex, enabling the authors to investigate how locally synthesized oestrogens regulate cortical migration in a sex-specific manner.
The Protective Effect of Aromatase on NSC-34 Cells with Stably Expressed hSOD1-G93A
Yan, Qi, Liu et al
Neuroscience (2019) 411, 37-46 DOI: 10.1016/j.neuroscience.2019.05.022
Yan, Qi, Liu et al
Neuroscience (2019) 411, 37-46 DOI: 10.1016/j.neuroscience.2019.05.022
AI Summary: OriGene products, specifically the Cyp19a1 mouse ORF cDNA plasmid (Cat NO: MR224509) and the empty PCMV6-Entry vector (Cat NO: PS100001), were used in this study to overexpress and serve as controls for aromatase in NSC-34 cells, enabling the researchers to investigate the protective role of aromatase against cell damage in an ALS cell model.
Snippet: The following plasmids were used in this study: Cyp19a1 mouse open reading frame (ORF) cDNA cloned into the PCMV6-Entry vector with C-terminal Myc-DDK-tag (GenBank ID: 13075, NO: NM_007810;Cat NO: MR224509;ORIGENE) and empty PCMV6-Entry vector;ORIGENE) and empty PCMV6-Entry vector with C-terminal Myc-DDK-tag (Cat NO: PS100001;ORIGENE), Cyp19a1 short hairpin RNA (ShRNA) (Cyp19a1-RNAi, target serial information: CTGGAGGATGACGTAATTG;starting position: 1171;GenBank ID: 13075, NO: NM_007810;Shanghai Genechem Co. …
Brain-synthesized estrogens regulate cortical migration in a sexually divergent manner
Sellers, Denley, Saito et al
BioRxiv (2019)
Sellers, Denley, Saito et al
BioRxiv (2019)
Showing 1-3 of 3 papers.
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