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Pgd (NM_001081274) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224503
Pgd (Myc-DDK-tagged) - Mouse phosphogluconate dehydrogenase (Pgd)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pgd or NM_001081274"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pgd
Synonyms 0610042A05Rik; AU019875; C78335
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224503 representing NM_001081274
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAAGCTGACATTGCACTGATCGGACTGGCTGTCATGGGCCAGAACTTAATTTTGAACATGAATG
ATCATGGATTTGTGGTCTGTGCTTTCAATAGGACAGTCTCCAAAGTCGATGACTTCTTGGCCAACGAGGC
GAAGGGCACCAAGGTGGTTGGTGCACAGTCCTTAAAGGACATGGTCTCCAAACTAAAGAAGCCCCGCCGG
GTCATCCTGCTTGTGAAGGCCGGGCAAGCCGTGGATGATTTCATCGAGAAATTAGTACCCTTGTTGGACA
CGGGTGACATCATCATCGATGGAGGAAATTCTGAATACCGGGACACGACAAGAAGATGCCGGGACCTCAA
GGCCAAGGGCATCTTGTTTGTGGGGAGCGGAGTCAGTGGTGGTGAGGAAGGGGCTCGGTACGGGCCGTCA
CTCATGCCAGGAGGGAACAAAGAGGCTTGGCCCCACATCAAGGCGATCTTCCAAGCCATCGCTGCAAAAG
TGGGAACCGGAGAACCCTGCTGTGACTGGGTGGGAGATGAGGGGGCGGGGCACTTTGTGAAGATGGTGCA
CAACGGGATAGAGTATGGAGACATGCAGCTCATCTGTGAGGCTTACCATTTGATGAAGGATGTTCTGGGC
ATGCGGCATGAGGAGATGGCTCAGGCATTTGAAGAATGGAACAAGACAGAGCTGGACTCATTCCTGATTG
AAATCACTGCTAACATTCTCAAGTACCGGGACACTGACGGCAAAGAGCTGTTGCCAAAGATCCGGGACAG
TGCTGGGCAGAAGGGCACTGGGAAGTGGACCGCCATCTCGGCGCTGGAGTACGGCATGCCCGTCACCCTC
ATTGGAGAAGCTGTCTTTGCTCGGTGCTTGTCCTCTCTGAAGGAGGAGCGGGTTCAGGCCAGCCAAAAGC
TGAAGGGTCCTAAGGTGGTCCAGCTGGAAGGCAGTAAGAAGTCATTCCTGGAGGACATCCGTAAGGCCCT
CTATGCTTCCAAGATCATCTCCTACGCCCAAGGCTTTATGCTGCTCAGACAGGCAGCCACTGAGTTTGGC
TGGACCCTCAATTATGGCGGCATTGCCCTGATGTGGAGAGGGGGCTGCATCATCCGAAGTGTGTTCCTGG
GAAAAATTAAAGATGCATTTGAGCGAAACCCAGAACTTCAGAACCTACTGCTAGATGACTTCTTTAAGTC
AGCAGTTGACAACTGCCAGGACTCCTGGCGGCGGGTGATCAGCACTGGGGTGCAAGCAGGCATTCCCATG
CCCTGCTTCACTACTGCCCTCTCCTTCTATGATGGGTACAGACACGAGATGCTGCCAGCAAACCTCATCC
AGGCTCAACGGGATTACTTTGGGGCTCACACCTATGAACTCTTAACCAAACCGGGAGAATTTATCCACAC
CAACTGGACGGGCCACGGGGGCAGTGTGTCATCCTCTTCATACAATGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224503 representing NM_001081274
Red=Cloning site Green=Tags(s)

MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKDMVSKLKKPRR
VILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPS
LMPGGNKEAWPHIKAIFQAIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLG
MRHEEMAQAFEEWNKTELDSFLIEITANILKYRDTDGKELLPKIRDSAGQKGTGKWTAISALEYGMPVTL
IGEAVFARCLSSLKEERVQASQKLKGPKVVQLEGSKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFG
WTLNYGGIALMWRGGCIIRSVFLGKIKDAFERNPELQNLLLDDFFKSAVDNCQDSWRRVISTGVQAGIPM
PCFTTALSFYDGYRHEMLPANLIQAQRDYFGAHTYELLTKPGEFIHTNWTGHGGSVSSSSYNA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081274
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081274.2
RefSeq Size 2224 bp
RefSeq ORF 1452 bp
Locus ID 110208
UniProt ID Q9DCD0
Cytogenetics 4 78.96 cM
MW 53.7 kDa
Summary Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081274" in other vectors (4)
SKU Description Size Price
MC216702 Pgd (untagged) - Mouse phosphogluconate dehydrogenase (Pgd), (10ug) 10 ug
$503.00
MG224503 Pgd (tGFP-tagged) - Mouse phosphogluconate dehydrogenase (Pgd), (10ug) 10 ug
$657.00
MR224503L3 Lenti ORF clone of Pgd (Myc-DDK-tagged) - Mouse phosphogluconate dehydrogenase (Pgd) 10 ug
$757.00
MR224503L4 Lenti ORF clone of Pgd (mGFP-tagged) - Mouse phosphogluconate dehydrogenase (Pgd) 10 ug
$757.00

Plasmid Map for MR224503

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224503
Circular map for MR224503

file-alt Citations

Pentose Phosphate Shunt Modulates Reactive Oxygen Species and Nitric Oxide Production ControllingTrypanosoma cruziin Macrophages
Koo, Szczesny, Wan et al
Front Immunol (2018) 9, 202 DOI: 10.3389/fimmu.2018.00202
AI Summary: OriGene contributed to this study by providing the mouse PGD siRNA (110208 locus), which was used to silence the PGD gene and investigate its role in the pentose phosphate pathway's regulation of reactive oxygen species and nitric oxide production in Trypanosoma cruzi-infected macrophages.
Snippet: Silencing of genes encoding for G6PD and PGD was performed by using specific siRNA duplexes directed against mouse G6PD (s66341, Thermo Scientific, 20 nM) and mouse PGD (110208 locus, OriGene, Rockville, MD, USA, 70 nM). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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