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Dnaic2 (NM_001034878) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224479
Dnaic2 (Myc-DDK-tagged) - Mouse dynein, axonemal, intermediate chain 2 (Dnaic2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $580.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnaic2
Synonyms b2b3405Clo; C030015H18Rik; Dnai2; Gm728
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224479 representing NM_001034878
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATCGTCTACGTGTACCTGAAGAAGCGCAGCGAATTTGGGAAGCAATGCAACTTCTCAGATCGCC
AGGCTGAGCTGAACATCGACATCTTACCCAACCCCGAGCTGGCCGCACTGTATGTGGAGCGGAATCCAGT
AGACACAGGCATCCAGTGCTCCGCCAGTATGTCGGAACACGAGGCCAACACCGAGCGGTTCGAGATGGAG
AGCTGTGGCGTTAATCATGTCGAGGGGGGCTGGCCCAAGGATGTGAACCCCCAGGAGCTGGAGCAGACCA
TCCGTTTCCGGAAGAAAGTGGAGAAGGATGAGAACTACATCAACGCCGTCATGCAGCTGGGCTCGATCAT
GGAGCACTGCATCAAGCAGAACAACGCCATCGACATCTACGAGGAATACTTTGATGACGAGGAGGCCGTG
GAGGTGACTGAAGAAGCCCCTTCGGCCAAGACCATCAATGTCTTCAGGGACCCTCAGGAGATCAAGCGGA
CAGCTACACACCTCTCCTGGCACCCTGATGGCAACAGGAAGCTGGCAGTAGCCTATTCCTGCTTGAAGTT
CCAGCGCGCACCCATGAGCATGAACTACGACTCCTACATCTGGGACCTGGAAAACCCCAACCGACCTGAG
ATTGCCCTGAAGCCGTTGTCTCCTCTCGTCACCCTGGAGTACAACCCCAAGGATTCCCACGTGCTCCTAG
GGGGCTGCTACAATGGGCAGATAGCCTGCTGGGATACCCGGAAGGGCAGCCTGGTGGCAGAGCTGTCCAC
CATCGAGTTCAGCCACCGAGACCCAGTGTATGGCACCATCTGGCTGCAGTCAAAGACAGGCACTGAGTGC
TTCTCAGCATCCACTGATGGGCAGGTCATGTGGTGGGACATCCGCAAGATCAGCGAGCCCATCGAAGTTG
TCATTATGGACATTTCCAGAAAGGAGCAGCTGGAGAACGCCCTGGGCGCCATCTCCTTGGAGTTCGAGTC
TACCTTGCCAACCAAGTTCATGGTGGGCACCGAACAGGGCATCGTCATCTCCTGCAACCGCAAGGCCAAG
ACGCAAGCAGAGAAGATTGTTTGCACCTTTTATGGCCACCACGGCCCCATCTATGCCCTCCAGAGAAACC
CCTTCTACCCAAAGAACTTTCTGACTGTTGGAGACTGGACAGCCCGCATCTGGTCTGAGGACAGTCGGGA
GTCATCGATCATGTGGACCAAGTACCACATGGCTTACCTCTCCGATGGAGCCTGGAGTCCTGTGAGACCA
GCCGTTTTCTTCACCACCAAGATGGATGGGACCCTGGACATCTGGGACTTGGTGTTCAAGCAGTGTGACC
CTGCCCTCAGCCTAAAGGTGTGTGATGACCCACTCTTCTGCCTCCGGGTTCAGGACAACGGGTGTCTCAT
CGCGTGTGGCTCCGAGCTCGGGACGACCACTCTGCTGGAGGTCTCCAGCAGTCTCTCCACTCTGCAGAGG
AATGAGAAGAACATAGCTTCTTCCATATTTGAACGTGAAACCCGGCGGGAAAAGATCCTGGAGGCCAGGC
ACCGTGAGATGCGACTGAAGGAGAAAGGCAAGGTGGAGGGCAAAGAGGATGACCAGAAGGAGGAGGAGGC
AGCCCTGGACCTTGATGAGCTAGTTGGCAAAGCAGAGGAGGAGTTCTTTGAAGTCATCTTCTCAGAGCTG
AAGAGGAAGGAGGCAGAGGCCTTGAAGAAGAAACCGAAGCCTAGGAAAAAATCAAGTGTCAAGGTGGAAG
CCGAAGAGGAAGTGGAAGAAAATGTTGGAGAGGAGGAAGAAGCAGGTGGCATAATAGGTATAGATGCAGT
TGAAGACATGAGTGAAGAAGCAGGTGAAGAGCAAGAGGATGTGCCCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224479 representing NM_001034878
Red=Cloning site Green=Tags(s)

MEIVYVYLKKRSEFGKQCNFSDRQAELNIDILPNPELAALYVERNPVDTGIQCSASMSEHEANTERFEME
SCGVNHVEGGWPKDVNPQELEQTIRFRKKVEKDENYINAVMQLGSIMEHCIKQNNAIDIYEEYFDDEEAV
EVTEEAPSAKTINVFRDPQEIKRTATHLSWHPDGNRKLAVAYSCLKFQRAPMSMNYDSYIWDLENPNRPE
IALKPLSPLVTLEYNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIEFSHRDPVYGTIWLQSKTGTEC
FSASTDGQVMWWDIRKISEPIEVVIMDISRKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAK
TQAEKIVCTFYGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLSDGAWSPVRP
AVFFTTKMDGTLDIWDLVFKQCDPALSLKVCDDPLFCLRVQDNGCLIACGSELGTTTLLEVSSSLSTLQR
NEKNIASSIFERETRREKILEARHREMRLKEKGKVEGKEDDQKEEEAALDLDELVGKAEEEFFEVIFSEL
KRKEAEALKKKPKPRKKSSVKVEAEEEVEENVGEEEEAGGIIGIDAVEDMSEEAGEEQEDVPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001034878
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001034878.3
RefSeq Size 2871 bp
RefSeq ORF 1872 bp
Locus ID 432611
UniProt ID A2AC93
Cytogenetics 11 E2
MW 71 kDa
Summary Part of the dynein complex of respiratory cilia.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001034878" in other vectors (4)
SKU Description Size Price
MC219890 Dnaic2 (untagged) - Mouse dynein, axonemal, intermediate chain 2 (Dnaic2), (10ug) 10 ug
$581.00
MG224479 Dnaic2 (tGFP-tagged) - Mouse dynein axonemal intermediate chain 2 (Dnaic2), (10ug) 10 ug
$780.00
MR224479L3 Lenti ORF clone of Dnaic2 (Myc-DDK-tagged) - Mouse dynein, axonemal, intermediate chain 2 (Dnaic2) 10 ug
$880.00
MR224479L4 Lenti ORF clone of Dnaic2 (mGFP-tagged) - Mouse dynein, axonemal, intermediate chain 2 (Dnaic2) 10 ug
$880.00

Plasmid Map for MR224479

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224479
Circular map for MR224479

file-alt Citations

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