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Retsat (NM_026159) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224429
Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $567.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Retsat
Synonyms 0610039N19Rik; C80029; MMT-7; Ppsig
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224429 representing NM_026159
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGATCACTGCTCTGCTGCTGGCCGTGCTGCTGCTGGTGATCCTCCACAGGGTCTACGTGGGCCTTT
ACGCTGCAAGTTCCCCGAACCCCTTCGCCGAGGATGTCAAGCGACCGCCTGAACCCCTGGTGACCGACAA
GGAGGCTAGGAAGAAAGTTCTCAAACAAGCTTTCTCAGTCAGCCGAGTACCAGAGAAGCTGGATGCAGTG
GTGATCGGCAGCGGCATTGGGGGACTGGCCTCAGCTGCGGTTCTAGCTAAAGCTGGCAAGAGAGTCCTTG
TGCTGGAACAACATACCAAGGCGGGCGGCTGTTGTCATACCTTTGGGGAAAATGGCCTTGAATTTGACAC
TGGAATTCATTATATTGGACGAATGCGGGAGGGCAACATTGGCCGTTTTATCTTGGACCAGATCACTGAA
GGGCAACTGGACTGGGCCCCCATGGCCTCCCCTTTTGACTTGATGATACTAGAAGGGCCCAATGGCCGAA
AGGAGTTCCCCATGTACAGTGGGAGGAAAGAATACATCCAGGGCCTTAAGAAGAAGTTCCCCAAGGAAGA
AGCTGTCATTGACAAGTACATGGAGTTGGTTAAGGTGGTGGCCCGTGGAGTCTCTCATGCAGTTCTACTC
AAGTTCCTCCCATTGCCCTTGACTCAGCTCCTCAGCAAGTTTGGGCTACTGACTCGTTTCTCTCCATTCT
GCCGAGCGTCTACGCAGAGCCTAGCTGAAGTCCTGCAGCAGCTTGGGGCTTCCCGTGAGCTCCAGGCTGT
TCTCAGCTACATCTTCCCCACTTACGGAGTAACTCCCAGCCACACCGCCTTTTCCTTGCATGCTCTGCTG
GTTGACCACTACATACAAGGGGCATATTACCCTCGAGGGGGTTCCAGTGAGATCGCCTTCCATACCATCC
CTTTGATTCAGCGGGCCGGGGGCGCTGTCCTCACCAGGGCCACTGTACAGAGTGTGCTGCTGGACTCAGC
TGGGAGAGCGTGTGGTGTCAGTGTGAAGAAGGGACAAGAGCTGGTGAACATCTACTGCCCAGTTGTCATC
TCCAATGCGGGAATGTTCAATACCTATCAGCACTTGTTGCCAGAGACTGTCCGCCATCTGCCAGATGTGA
AGAAGCAGCTGGCGATGGTAAGGCCTGGTCTGAGCATGCTCTCAATCTTCATCTGTCTGAAAGGCACCAA
GGAGGACCTGAAGCTTCAGTCCACCAACTACTATGTTTATTTTGACACAGACATGGACAAAGCGATGGAG
CGCTATGTCTCTATGCCCAAGGAAAAGGCTCCAGAACACATTCCCCTTCTCTTCATTGCCTTCCCATCAA
GCAAGGATCCAACCTGGGAGGAGCGATTCCCAGACCGATCCACAATGACTGCGCTGGTACCCATGGCCTT
TGAATGGTTCGAGGAGTGGCAGGAGGAGCCAAAGGGCAAGCGTGGTGTTGACTATGAGACCCTCAAAAAT
GCCTTCGTGGAAGCCTCTATGTCGGTGATCATGAAACTGTTCCCACAGCTGGAGGGCAAGGTGGAGAGTG
TGACTGGAGGGTCACCACTGACCAACCAGTACTATCTGGCTGCACCCCGAGGAGCTACCTATGGAGCTGA
CCATGACTTGGCTCGGCTGCATCCTCATGCAATGGCTTCCATAAGAGCCCAAACCCCCATCCCCAACCTC
TACCTGACAGGCCAAGATATCTTCACCTGTGGGCTGATGGGGGCCCTGCAGGGGGCCTTGCTGTGCAGCA
GTGCCATCCTGAAACGGAACTTGTACTCAGATCTGCAGGCTCTTGGCTCAAAGGTCAAGGCACAAAAGAA
GAAGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224429 representing NM_026159
Red=Cloning site Green=Tags(s)

MWITALLLAVLLLVILHRVYVGLYAASSPNPFAEDVKRPPEPLVTDKEARKKVLKQAFSVSRVPEKLDAV
VIGSGIGGLASAAVLAKAGKRVLVLEQHTKAGGCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITE
GQLDWAPMASPFDLMILEGPNGRKEFPMYSGRKEYIQGLKKKFPKEEAVIDKYMELVKVVARGVSHAVLL
KFLPLPLTQLLSKFGLLTRFSPFCRASTQSLAEVLQQLGASRELQAVLSYIFPTYGVTPSHTAFSLHALL
VDHYIQGAYYPRGGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVSVKKGQELVNIYCPVVI
SNAGMFNTYQHLLPETVRHLPDVKKQLAMVRPGLSMLSIFICLKGTKEDLKLQSTNYYVYFDTDMDKAME
RYVSMPKEKAPEHIPLLFIAFPSSKDPTWEERFPDRSTMTALVPMAFEWFEEWQEEPKGKRGVDYETLKN
AFVEASMSVIMKLFPQLEGKVESVTGGSPLTNQYYLAAPRGATYGADHDLARLHPHAMASIRAQTPIPNL
YLTGQDIFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVKAQKKKM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026159
ORF Size 1827 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026159.5
RefSeq Size 1942 bp
RefSeq ORF 1830 bp
Locus ID 67442
UniProt ID Q64FW2
Cytogenetics 6 C1
MW 67.8 kDa
Summary Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol (PubMed:15358783, PubMed:17253779, PubMed:19139408). Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers (PubMed:15358783). May play a role in the metabolism of vitamin A (PubMed:15358783, PubMed:17253779). Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP (PubMed:28855500). Required for adipocyte differentiation in a 3T3-L1 cell culture model (PubMed:19139408). This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT (PubMed:19940255).UniProtKB/Swiss-Prot Function
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"NM_026159" in other vectors (4)
SKU Description Size Price
MC219629 Retsat (untagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat), (10ug) 10 ug
$625.00
MG224429 Retsat (tGFP-tagged) - Mouse retinol saturase (all trans retinol 1314 reductase) (Retsat), (10ug) 10 ug
$767.00
MR224429L3 Lenti ORF clone of Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) 10 ug
$867.00
MR224429L4 Lenti ORF clone of Retsat (mGFP-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) 10 ug
$867.00

Plasmid Map for MR224429

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224429
Circular map for MR224429

file-alt Citations

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