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Phtf1 (NM_001163467) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224329
Phtf1 (Myc-DDK-tagged) - Mouse putative homeodomain transcription factor 1 (Phtf1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $697.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phtf1
Synonyms AU041898; AW049785; Phft; Phtf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224329 representing NM_001163467
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAAATGAGAGAGATGCTATATCATGGTATCAGAAGAAGATTGGAGCATATGATCAGCAGATAT
GGGAAAAGTCAATCGAACAGACTCAGATTAAGGGTTTCAAAAACAAACCAAAAAAAATGGGCCATATAAA
ACCAGACTTGATTGATGTTGACTTAATCAGAGGCTCAACATTTGCCAAAGCCAAGCCTGAGATTCCATGG
ACCTCGCTCACTCGGAAGGGGCTTGTTCGAGTGGTGTTTTTCCCGTTGTTCAGCAGCTGGTGGATTCAGG
TTACCTCTCTCAGAATCTTTGTTTGGTTGCTGCTACTTTACCTCATGCAAGTCACAGCGGTTGTGTTGTA
TCTCCTGATGCCTATTGTGAGCGCCAGTGAAGTGCTTGGACCCTTGTGCCTTATGCTACTCATGGGAACT
GTCCACTGTCAGATTGTGTCGACTCAGATAACAAGGCCGTCAGGAAACAATGGAAATCGGAGAAGAAGAA
AATTACGAAAAACTGTAAATGGTGATGGGAGCCGAGACAATGGAAATAACTCCCCTGATAAAGTCAGAGC
AGTAGAAACTCTGGAGTCTGCATCCAGTGTTGGTGGTTTTTGGGGGACTCTCTTTGGCAACAGGATTAAA
AGGGTGAAATTAGTATCTAACAAAGGTACTGAAACTGACAATGACTCAGGCTGTTTCCATCCTATCCTTA
AGAAGAGACAGGGTCGACCCGAGATCAGAATGTGGCAAGCAAGAGAGAAAGCGAAGGTTTCGGATGGAGA
AAAGTGCCGTAGGGAGGCTTATAGGCGTTTGGGTAATGGAATTTCAGACGATCTGTCAAGTGAGGAGGAT
GGTGAAGCACGGACACAGATGATATTATTGCGTAGGAGTGTGGAAGGGGCGTCAAGTGACAATGGTTATG
AAGTTAAGAATAGAAGGTCAATACTTTCAAGGCACCTAAATTCTCAGGTAAAGAAAACCACAAGGTGGTG
TCATATTGTTCGGGATTCAGATAGTCTGGCTGAATCAGAGTTTGAATCAGCAGTCTTCAGCCAGGGTTCT
AGATCTGGTATGAGTGGTGGCTCTCGGAGCCTTAACTTGTCAAGAAGAGACTCAGAAAGCACCCGACATG
ACTCCGAGACTGAGGATATGTTATGGGATGACCTTCTCCATGGTCCAGAGTGCCGGTCATCTGTCACCAG
TGACAGTGAGGGGGCTCATGTAAATACCATTCATTCAGGGACCAAACGTGACCCCAAAGAAGATGTTTTT
CAGCAGAACCATTTATTCTGGCTTCAGAATTCAAGTCCTGCCTCTGAGCGAGTTAGTGCGATAATCTGGG
AAGGAAACGAGTGCAAAAAGATGGACATGTCTGTGTTGGAGATAAGCGGCATCATCATGAGCAGGGTCAA
TGCATACGAGCAAGGTGTTGGCTATCAGATGCTGGGGAATGCAGTCACCGTTGGCTTAGCACTTTTCCCA
TTTTTATATCGACTTTTCCGAGAGAAGAGCTTTGACCAACTAAAGTCCATCTCAGCAGAGGAGGTTCTGA
CCCTCTTCTGTGGTGCGCCACCCGTCACACCTGTTGTTATTTTGTCTATAATTAATTTTATTGAAAGATT
GTGTCTTACGTGGATGTTTTTTTTCATGATGTGTGTGGCAGAGAGAACATATAAGCAGAGATTTTTATTT
GCAAAACTCTTCAGCCATATTACTTCTGCCAGGAAGGCCAGGAAATATGAAATACCTCATTTCAGACTTA
AAAAGGTGGAGAACATTAAAATATGGTTATCCCTGCGCTCCTATCTAAAGAGGCGGGGTCCACAGCGCTC
AGTGGACGTGGTTGTGTCCTCCGTCTTCTTACTGACACTTTCAATTGCTTTCATTTGTTGTGCACAGGTT
CTTCAAGGTCACAAGACATTCCTGAATGATGCTTACAACTGGGAGTTCTTGATCTGGGAAACAGCATTGC
TGCTGTTTTTGCTTCGCCTGGCCTCACTGGGGTCAGAAACCAACAAGAAGTACAGCAATGTTTCCATCCT
GCTCACTGAACAGATTAATTTATATCTTAAAATGGAAAAAAAGCCAAATAAGAAAGAACAGCTCACGCTA
GTGAATAATGTATTGAAGCTGTCCACAAAGCTGTTAAAGGAGCTGGACACACCATTTAGACTCTATGGAC
TGACCATGAACCCCTTAATCTACAACATCACAAGAGTAGTCATCCTTTCTGCTGTCTCTGGAGTTATAAG
CGACCTTCTAGGATTCAACATAAGACTGTGGAAAATTAAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224329 representing NM_001163467
Red=Cloning site Green=Tags(s)

MASNERDAISWYQKKIGAYDQQIWEKSIEQTQIKGFKNKPKKMGHIKPDLIDVDLIRGSTFAKAKPEIPW
TSLTRKGLVRVVFFPLFSSWWIQVTSLRIFVWLLLLYLMQVTAVVLYLLMPIVSASEVLGPLCLMLLMGT
VHCQIVSTQITRPSGNNGNRRRRKLRKTVNGDGSRDNGNNSPDKVRAVETLESASSVGGFWGTLFGNRIK
RVKLVSNKGTETDNDSGCFHPILKKRQGRPEIRMWQAREKAKVSDGEKCRREAYRRLGNGISDDLSSEED
GEARTQMILLRRSVEGASSDNGYEVKNRRSILSRHLNSQVKKTTRWCHIVRDSDSLAESEFESAVFSQGS
RSGMSGGSRSLNLSRRDSESTRHDSETEDMLWDDLLHGPECRSSVTSDSEGAHVNTIHSGTKRDPKEDVF
QQNHLFWLQNSSPASERVSAIIWEGNECKKMDMSVLEISGIIMSRVNAYEQGVGYQMLGNAVTVGLALFP
FLYRLFREKSFDQLKSISAEEVLTLFCGAPPVTPVVILSIINFIERLCLTWMFFFMMCVAERTYKQRFLF
AKLFSHITSARKARKYEIPHFRLKKVENIKIWLSLRSYLKRRGPQRSVDVVVSSVFLLTLSIAFICCAQV
LQGHKTFLNDAYNWEFLIWETALLLFLLRLASLGSETNKKYSNVSILLTEQINLYLKMEKKPNKKEQLTL
VNNVLKLSTKLLKELDTPFRLYGLTMNPLIYNITRVVILSAVSGVISDLLGFNIRLWKIKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163467
ORF Size 2283 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163467.1, NP_001156939.1
RefSeq Size 2936 bp
RefSeq ORF 2286 bp
Locus ID 18685
UniProt ID Q9QZ09
Cytogenetics 3 F2.2
MW 86.8 kDa
Summary May play a role in transcription regulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163467" in other vectors (4)
SKU Description Size Price
MC221372 Phtf1 (untagged) - Mouse putative homeodomain transcription factor 1 (Phtf1), transcript variant 2, (10ug) 10 ug
$767.00
MG224329 Phtf1 (tGFP-tagged) - Mouse putative homeodomain transcription factor 1 (Phtf1) transcript variant 2, (10ug) 10 ug
$897.00
MR224329L3 Lenti ORF clone of Phtf1 (Myc-DDK-tagged) - Mouse putative homeodomain transcription factor 1 (Phtf1), transcript variant 2 10 ug
$997.00
MR224329L4 Lenti ORF clone of Phtf1 (mGFP-tagged) - Mouse putative homeodomain transcription factor 1 (Phtf1), transcript variant 2 10 ug
$997.00

Plasmid Map for MR224329

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224329
Circular map for MR224329

file-alt Citations

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