Skint2 (NM_177865) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224277
Skint2 (Myc-DDK-tagged) - Mouse selection and upkeep of intraepithelial T cells 2 (Skint2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Skint2
Synonyms A430070M19; B7S3; OTTMUSG00000008540; RP23-170H12.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224277 representing NM_177865
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCTACAGGAGTACTTCTCTGTGTTGTCTTACACTTCCTCCAGATGGTAACACAGAGTTCAGAAA
AATTCACAGTGACTGGCTTACAGAGGCCAGTCTTGGCACCATTGGGTGGAAATGTTGAACTGAGTTGTCA
GTTGTCTCCGCCACAACAAGCACAGCATATGGAGATTCGTTGGTTTCGAAATCGCTATAGAGAGCCTGTG
TACCTGTACAGGAATGGTAAAGACCTGCATGGAGAAACTATCTCCAAGTATGTGGAGCGGACTGAACTCC
TGAAAGATGACATTGGAAAGGGGAAGGTGACCCTCAGGATCTTTAAACTGACAGCTGATGATGATGGGTC
ATACCACTGTGTCTTCAAAGTTGGAGAGTTCTATGAAGAGCATATCACAGAAATCAAGGTCACAGCCACA
AGCTCAGTCATGTACATTCTAATGCAGCCCCCTAATATCAAAGGTGTGATGTTGGAGTGTCATTCAGGAG
GTTGGTTCCCACAGCCTCATATGGAATGGAGAGACAACAAGGGAAATATCATTCCAGCTACATCAAAGGC
CCATTCACAGGATGAAAACAAATTGTTCAACATGACAATGACCCTTCTTATTGAAGCCAGCTCCCACAGG
AGTATCACTTGCTATCTTCAAAACCTTCTAACTCACCAAGAAGAAAGCATAAGTATTGTCCTATCAGGTG
AACTATTTTCATGGAAACGTGTTTGGATAATGATTCTGACTACAATAGGATTCATGATGATAGCCTTCTG
CATGACTTATTGTGTTCAGCAACATCTCTTATATGGTACCTTCTCTAAGGGGAAATGTCATTGGTTAAAG
AGTACAATGATATTCATGTTTTCAGTGATTGCAGTTACAGGAGTCATGCTCATTTTGCATCTAAAACAAA
GAGGTCTTATGGAAATTATTTATGAATGCAGAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224277 representing NM_177865
Red=Cloning site Green=Tags(s)

MGATGVLLCVVLHFLQMVTQSSEKFTVTGLQRPVLAPLGGNVELSCQLSPPQQAQHMEIRWFRNRYREPV
YLYRNGKDLHGETISKYVERTELLKDDIGKGKVTLRIFKLTADDDGSYHCVFKVGEFYEEHITEIKVTAT
SSVMYILMQPPNIKGVMLECHSGGWFPQPHMEWRDNKGNIIPATSKAHSQDENKLFNMTMTLLIEASSHR
SITCYLQNLLTHQEESISIVLSGELFSWKRVWIMILTTIGFMMIAFCMTYCVQQHLLYGTFSKGKCHWLK
STMIFMFSVIAVTGVMLILHLKQRGLMEIIYECRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177865
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177865.4, NP_808533.1
RefSeq Size 1678 bp
RefSeq ORF 948 bp
Locus ID 329919
Cytogenetics 4
MW 36.2 kDa
Summary May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177865" in other vectors (4)
SKU Description Size Price
MC214571 Skint2 (untagged) - Mouse selection and upkeep of intraepithelial T cells 2 (Skint2), (10ug) 10 ug
$330.00
MG224277 Skint2 (tGFP-tagged) - Mouse selection and upkeep of intraepithelial T cells 2 (Skint2), (10ug) 10 ug
$500.00
MR224277L3 Lenti ORF clone of Skint2 (Myc-DDK-tagged) - Mouse selection and upkeep of intraepithelial T cells 2 (Skint2) 10 ug
$600.00
MR224277L4 Lenti ORF clone of Skint2 (mGFP-tagged) - Mouse selection and upkeep of intraepithelial T cells 2 (Skint2) 10 ug
$600.00

Plasmid Map for MR224277

Download sequence for

Cloning scheme for MR224277

Circular map for MR224277

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