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Gucy2c (NM_001127318) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR224108
Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $933.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Gucy2c or NM_001127318"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gucy2c
Synonyms AI893437; GC-C; Gcc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224108 representing NM_001127318
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCACTGCTGGGCTTGGCTGTGCGGTTACTGCTCTTCCAGCCCGCGCTGATGGTGTTCTGGGCCT
CTCAGGTGAGGCAGAACTGCCGCAATGGCAGCTACGAGATCAGCGTCCTGATGATGGACAACTCAGCCTA
CAAAGAACCTATGCAAAACCTGAGGGAGGCTGTGGAGGAAGGACTGGACATAGTGCGAAAGCGCCTGCGT
GAAGCCGACCTAAATGTGACTGTGAACGCGACTTTCATCTACTCCGACGGTCTGATTCATAAGTCAGGTG
ACTGCCGGAGCAGCACCTGTGAAGGCCTTGACCTACTCAGGGAGATTACAAGAGATCATAAGATGGGCTG
CGCCCTCATGGGGCCCTCGTGCACGTATTCCACCTTCCAGATGTACCTCGACACAGAGTTGAACTATCCC
ATGATTTCCGCTGGAAGTTATGGATTGTCCTGTGACTATAAGGAAACCCTAACCAGGATCCTGCCTCCAG
CCAGGAAGCTGATGTACTTCTTGGTCGATTTCTGGAAAGTCAACAATGCATCTTTCAAACCCTTTTCCTG
GAACTCTTCGTATGTTTACAAGAATGGATCGGAACCTGAAGATTGTTTCTGGTACCTCAATGCTCTGGAG
GCTGGGGTGTCCTATTTTTCTGAGGTGCTCAACTTCAAGGATGTACTGAGACGCAGCGAACAGTTCCAGG
AAATCTTAACAGGCCATAACAGAAAGAGCAATGTGATTGTTATGTGTGGCACGCCAGAAAGCTTCTATGA
TGTGAAAGGTGACCTCCAAGTGGCTGAAGATACTGTTGTCATCCTGGTAGATCTGTTCAGTAACCATTAC
TTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGT
CCACCTCAAACACCTCTGTCGCCGAGAGGTTTTCATCGGGGAGAAGTGACTTTTCTCTCGCTTACTTGGA
GGGAACCTTGCTATTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAAAATGTCACGGGTCCCAAG
TTTGCTCGTGCATTCAGGAATCTCACTTTTCAAGGCTTTGCAGGACCTGTGACTCTGGATGACAGTGGGG
ACATTGACAACATTATGTCCCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGAAGTA
TGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTC
CCCAATGACGTTCCTGGGCTGGGCCCTCAAATCCTGATGATTGCCGTCTTCACGCTCACGGGGATCCTGG
TAGTTCTGCTGCTGATTGCCCTCCTCGTGCTGAGAAAATACAGAAGAGATCATGCACTTCGACAGAAGAA
ATGGTCCCACATTCCTTCTGAAAACATCTTTCCTCTGGAGACCAACGAGACCAACCACATCAGCCTGAAG
ATTGACGATGACAGGAGACGAGACACAATCCAGAGAGTGCGACAGTGCAAATACGACAAGAAGAAAGTGA
TTCTGAAAGACCTCAAGCACAGCGACGGGAACTTCAGTGAGAAGCAGAAGATAGACCTGAACAAGTTGCT
GCAGTCTGACTACTACAACCTGACTAAGTTCTACGGCACCGTGAAGCTGGACACCAGGATCTTTGGGGTG
GTTGAGTACTGCGAGAGGGGATCCCTCCGGGAAGTGTTAAACGACACAATTTCCTACCCTGACGGCACGT
TCATGGATTGGGAGTTTAAGATCTCTGTCTTAAATGACATCGCTAAGGGGATGTCCTACCTGCACTCCAG
TAAGATTGAAGTCCACGGGCGTCTCAAATCCACCAACTGCGTGGTGGACAGCCGCATGGTGGTGAAGATC
ACCGACTTTGGGTGCAATTCCATCCTGCCTCCAAAAAAAGACCTGTGGACGGCCCCGGAGCACCTGCGCC
AGGCCACCATCTCTCAGAAAGGAGACGTGTACAGCTTCGCCATCATTGCCCAGGAGATCATCCTCCGTAA
GGAGACTTTTTACACGCTGAGCTGTCGGGATCACAATGAGAAGATTTTCAGAGTGGAAAATTCATACGGG
AAACCTTTCCGCCCAGACCTCTTCCTGGAGACTGCAGATGAGAAGGAGCTGGAGGTCTATCTACTTGTCA
AAAGCTGTTGGGAGGAGGATCCAGAAAAGAGGCCAGATTTCAAGAAAATCGAGAGCACACTGGCCAAGAT
ATTTGGCCTTTTCCATGACCAGAAAAACGAGTCTTACATGGACACCTTGATCCGACGTCTCCAGCTGTAC
TCTCGAAACCTGGAACATCTGGTGGAGGAAAGGACTCAGCTGTACAAGGCGGAGAGGGACAGGGCTGACC
ACCTTAACTTCATGCTCCTCCCACGGCTGGTGGTAAAGTCACTGAAGGAGAAAGGCATCGTGGAGCCAGA
GCTGTACGAAGAAGTCACAATCTACTTCAGTGACATTGTGGGCTTCACCACCATCTGCAAGTATAGCACG
CCCATGGAGGTGGTGGACATGCTCAACGACATCTACAAGAGCTTTGACCAGATTGTGGACCACCATGACG
TCTACAAGGTAGAAACCATCGGTGACGCCTACGTGGTGGCCAGCGGTCTGCCTATGAGAAACGGCAACCG
ACACGCGGTAGACATTTCCAAGATGGCCTTGGACATCCTCAGCTTCATAGGGACCTTTGAGTTGGAGCAT
CTCCCTGGCCTCCCCGTGTGGATCCGCATTGGAGTTCATTCTGGGCCCTGCGCTGCTGGTGTTGTGGGGA
TCAAGATGCCTCGCTATTGCCTGTTTGGAGACACTGTCAACACTGCCTCCAGGATGGAATCCACCGGCCT
CCCCTTGAGGATTCACATGAGCAGCTCCACCATAACCATCCTGAAGAGAACGGATTGCCAGTTCCTGTAT
GAAGTGAGGGGAGAAACCTACTTAAAGGGAAGAGGGACAGAGACCACATACTGGCTGACTGGGATGAAGG
ACCAAGAATACAACCTGCCATCCCCACCGACAGTGGAGAACCAACAGCGTCTGCAGACTGAGTTCTCAGA
CATGATCGTTAGCGCCTTACAGAAAAGACAGGCCTCGGGCAAGAAGAGCCGGAGGCCCACTCGGGTGGCC
AGCTACAAGAAAGGCTTTCTGGAATACATGCAGCTGAACAATTCAGACCACGATAGCACCTATTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR224108 representing NM_001127318
Red=Cloning site Green=Tags(s)

MTSLLGLAVRLLLFQPALMVFWASQVRQNCRNGSYEISVLMMDNSAYKEPMQNLREAVEEGLDIVRKRLR
EADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDHKMGCALMGPSCTYSTFQMYLDTELNYP
MISAGSYGLSCDYKETLTRILPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALE
AGVSYFSEVLNFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHY
FEENTTAPEYMDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGHMLQTFLENGENVTGPK
FARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYKVLMKYDTHKNKTIPVAENPNFIWKNHKL
PNDVPGLGPQILMIAVFTLTGILVVLLLIALLVLRKYRRDHALRQKKWSHIPSENIFPLETNETNHISLK
IDDDRRRDTIQRVRQCKYDKKKVILKDLKHSDGNFSEKQKIDLNKLLQSDYYNLTKFYGTVKLDTRIFGV
VEYCERGSLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSRMVVKI
TDFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFAIIAQEIILRKETFYTLSCRDHNEKIFRVENSYG
KPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKIESTLAKIFGLFHDQKNESYMDTLIRRLQLY
SRNLEHLVEERTQLYKAERDRADHLNFMLLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYST
PMEVVDMLNDIYKSFDQIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFIGTFELEH
LPGLPVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTITILKRTDCQFLY
EVRGETYLKGRGTETTYWLTGMKDQEYNLPSPPTVENQQRLQTEFSDMIVSALQKRQASGKKSRRPTRVA
SYKKGFLEYMQLNNSDHDSTYF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001127318
ORF Size 3216 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001127318.1, NP_001120790.1
RefSeq Size 3951 bp
RefSeq ORF 3219 bp
Locus ID 14917
UniProt ID Q3UWA6
Cytogenetics 6 66.67 cM
MW 123.7 kDa
Summary Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001127318" in other vectors (6)
SKU Description Size Price
MC223446 Gucy2c (untagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1, (10ug) 10 ug
$1,027.00
MG224108 Gucy2c (tGFP-tagged) - Mouse guanylate cyclase 2c (Gucy2c) transcript variant 1, (10ug) 10 ug
$789.00 $1,133.00
MR224108L1 Lenti ORF clone of Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1 10 ug
$1,233.00
MR224108L2 Lenti ORF clone of Gucy2c (mGFP-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1 10 ug
$1,233.00
MR224108L3 Lenti ORF clone of Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1 10 ug
$1,233.00
MR224108L4 Lenti ORF clone of Gucy2c (mGFP-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1 10 ug
$1,233.00

Plasmid Map for MR224108

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR224108
Circular map for MR224108

file-alt Citations

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