Jmjd4 (NM_178659) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224074
Jmjd4 (Myc-DDK-tagged) - Mouse jumonji domain containing 4 (Jmjd4), transcript variant 1
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jmjd4
Synonyms 6430559I23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224074 representing NM_178659
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCGGGAGACACGCACCTTTGCCGAGCGGTACTACCGGGACCTCCGAGACCCAGTCCCTAGCGGCG
GTGGCGGCCCCACACCCAGCGGCGTGACCTTCATTCAGACCCCGAACGCCTTCTCCTATGCTGACTTCGT
GAAGGGCTTCCTGCTGCCTAACCTGCCCTGCGTGTTTTCTAGCGCCTTCACCGAGGGCTGGGGCAGCAGG
CGGCGCTGGGTGACCTCGGAGGGAAAGCCTGACTTCGAGTATCTGCAGCAGAAGTATGGAGACGCGGTTG
TACCGGTTGCCAACTGTGGAGTGCGAGAATACAATTCAAACCCCAAGGAGCACATGTCCTTCCGCGACTA
TATCAGTTACTGGAAGGACTACATCCAAGGGAGTTACTCCTCTTCGCGGGGCTGCCTCTACCTCAAGGAC
TGGCATCTGTGCAGAGACTCCTTGGTGAATGACTTGGAAGACATATTCACCCTGCCCGTGTACTTCTCGT
CAGACTGGCTGAATGAGTTCTGGGACGTCCTCAATGTGGATGACTATCGTTTTGTCTATGCAGGCCCCAG
GGGCACCTGGTCTCCCTTCCATGCAGACATCTTCCGCTCCTTCAGCTGGTCAGTCAACATCTGTGGGAAG
AAGAAATGGTTGTTCTTCCCGCCAGGCGAGGAGGAAGCCCTCCGGGACTGCCATGGTAACCTGCCCTATG
ATGTGACTTCCACTGAGCTCCTGGACACCCACCTGTATCCCAAGATCCAGCACCACAGCCTTCCCATTGA
AGTCATACAGGAGCCTGGTGAGATGGTGTTTGTGCCCAGCGGGTGGCACCATCAAGTGTACAACCTGGAT
GACACCATTTCTATTAACCACAACTGGGTCAATGGCTGCAACCTGCCAAACATGTGGCACTTCCTGCAGC
AAGAGCTCCAGGCTGTGCAGCATGAGGTCGAAGAATGGAAGGACTCCATGCCTGACTGGCACCACCACTG
CCAGGTCATCATGAAGTCCTGCACAGGGATCAATTTTGAAGAATTTTACCATTTCCTCAAAGTCATCGCC
GAGAAGAGGCTCCTTGTCCTGGAGCAAGGACTGAAGGGGGATTCAGGGGACAGCCGGAGCCTAGACCTGG
GCCTACAGCAGGCTGCATTTGATATTGGCCGCCTTGCAGATGTGCTGGCCTCTGTGGTTGTGAATCCTGA
CTTTCAGAGAGTGGACACCAGCGCATTCTCACCACAGCCAGAAGAGTTGCTTCAGCAGCTGGAGGACGCA
GTGGCTGCTGCAGAGGCCCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224074 representing NM_178659
Red=Cloning site Green=Tags(s)

MDRETRTFAERYYRDLRDPVPSGGGGPTPSGVTFIQTPNAFSYADFVKGFLLPNLPCVFSSAFTEGWGSR
RRWVTSEGKPDFEYLQQKYGDAVVPVANCGVREYNSNPKEHMSFRDYISYWKDYIQGSYSSSRGCLYLKD
WHLCRDSLVNDLEDIFTLPVYFSSDWLNEFWDVLNVDDYRFVYAGPRGTWSPFHADIFRSFSWSVNICGK
KKWLFFPPGEEEALRDCHGNLPYDVTSTELLDTHLYPKIQHHSLPIEVIQEPGEMVFVPSGWHHQVYNLD
DTISINHNWVNGCNLPNMWHFLQQELQAVQHEVEEWKDSMPDWHHHCQVIMKSCTGINFEEFYHFLKVIA
EKRLLVLEQGLKGDSGDSRSLDLGLQQAAFDIGRLADVLASVVVNPDFQRVDTSAFSPQPEELLQQLEDA
VAAAEAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178659
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178659.6, NP_848774.1
RefSeq Size 4445 bp
RefSeq ORF 1284 bp
Locus ID 194952
UniProt ID Q8BFT6
Cytogenetics 11 B1.3
MW 49.2 kDa
Summary Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1 (By similarity). Not essential for embryonic stem cell (ESC) maintenance and the embryonic and postnatal development (PubMed:27147518).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178659" in other vectors (4)
SKU Description Size Price
MC212467 Jmjd4 (untagged) - Mouse jumonji domain containing 4 (Jmjd4), transcript variant 1, (10ug) 10 ug
$503.00
MG224074 Jmjd4 (tGFP-tagged) - Mouse jumonji domain containing 4 (Jmjd4), (10ug) 10 ug
$657.00
MR224074L3 Lenti ORF clone of Jmjd4 (Myc-DDK-tagged) - Mouse jumonji domain containing 4 (Jmjd4), transcript variant 1 10 ug
$757.00
MR224074L4 Lenti ORF clone of Jmjd4 (mGFP-tagged) - Mouse jumonji domain containing 4 (Jmjd4), transcript variant 1 10 ug
$757.00

Plasmid Map for MR224074

Download sequence for

Cloning scheme for MR224074

Circular map for MR224074

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